Antibody Shared Resources

抗体共享资源

• 批准号: 8473668
• 负责人: BRUCE W. STILLMAN
• 金额: $ 18.33万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8473668
• 关键词: Animals; Antibodies; Antibody Formation; Antigens; Ascites; Binding; Biological Assay; Biological Markers; Cancer Center; Cancer Center Support Grant; Cancer Center at Cold Spring Harbor Laboratory; Cell Culture Techniques; Charge; Clone Cells; Detection; Diagnostic; Freezing; Hemorrhage; Human Resources; Hybridomas; Immunization; Individual; Instruction; Knowledge; Laboratories; Liquid substance; Malignant Neoplasms; Monoclonal Antibodies; Oncogenes; Process; Production; Proteins; Reagent; Research; Resource Sharing; Resources; Sampling; Scientist; Services; Site; Staging; Techniques; Testing; Time; Tumor Suppressor Genes; animal facility; anticancer research; cancer genomics; cost; cost effective; in vivo; large scale production; meetings; monoclonal antibody production; novel; polyclonal antibody; screening; therapeutic development

PROJECT SUMMARY (See instructions): The Antibody Shared Resource provides a central site for the production of monoclonal and polyclonal antibodies by CSHL Cancer Center personnel. The purpose of this Shared Resource is to provide the resources and expertise to generate these valuable reagents in a highly efficient and cost-effective manner. In the ease of monoclonal antibodies, CSHL Cancer Center scientists provide specific antigens, and the Shared Resource personnel handle all stages of immunization, fusion, primary screening of hybridomas, cell culture, single-cell cloning, freezing and storage of hybridoma lines, and large-scale production of monoclonal antibodies in cell culture or in vivo. Immunization, test bleeds, and production of ascites fluids are coordinated with the personnel of the Animal Shared Resource. Specialized monoclonal antibody screens are closely coordinated with individual scientists. Polyclonal antibody production is outsourced, but the Shared Resource personnel are in charge of processing and distribution of samples, thus reducing the costs and maximizing efficiency. The Shared Resource also tests and implements new techniques, reagents, and assay improvements related to the different stages of antibody production.

项目概述（见说明）：