A High Throughput Assay for MHC-Peptide Interactions

MHC-肽相互作用的高通量测定

• 批准号: 8593060
• 负责人: MARK S. CHEE
• 金额: $ 22.38万
• 依托单位: PROGNOSYS BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8593060
• 关键词: Address; Affinity; Allergens; Antigens; Autoantigens; Autoimmune Diseases; Binding; Biological; Biological Assay; Blood; Blood specimen; Cell Line; Clinical; Complementary DNA; Complex; Computer Simulation; DNA Sequence; Detection; Epitopes; Goals; HLA-DR3 Antigen; Histocompatibility; Human; Immune; Immune response; Immune system; Individual; Infectious Diseases Research; Influenza; Literature; Major Histocompatibility Complex; Measures; Monitor; Peptides; Phase; Proteomics; Reaction; Relative (related person); Reporting; Research; Solutions; System; T-Cell Receptor; T-Lymphocyte; T-Lymphocyte Epitopes; Technology; Testing; Therapeutic; Vaccine Design; Variant; base; cDNA Probes; design; direct application; high throughput screening; immunogenicity; interest; new technology; next generation sequencing; novel; novel strategies; pathogen; programs; public health relevance; response; tool; tumor; vaccine candidate; vaccine development

DESCRIPTION (provided by applicant): There is a need for new proteomics technologies to enable multiplexed assays of immune system response. We aim to develop a novel technology for this application that is based on simultaneously measuring the binding of many customizable in silico-defined peptides. This technology could be applied to identify epitopes from pathogens, autoantigens, allergens or tumors for use in vaccine design and monitoring of immune responses.

描述（由申请人提供）：需要新的蛋白质组学技术来实现免疫系统反应的多重分析。我们的目标是为这一应用开发一种新技术，该技术基于同时测量许多可定制的硅定义肽的结合。该技术可用于从病原体、自身抗原、过敏原或肿瘤中识别抗原表位，用于疫苗设计和免疫反应监测。