Multiplexed Protein Assays

多重蛋白质检测

• 批准号: 8648596
• 负责人: MARK S. CHEE
• 金额: $ 35万
• 依托单位: PROGNOSYS BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8648596
• 关键词: Address; Antibodies; Atlases; Biological Assay; Brain; Cells; Communication; Complex; Coupled; DNA; Data; Detection; Development; Diagnostic; Diagnostics Research; Disease; Fluorescence; Formalin; Gene Proteins; Generations; Genes; Genetic Transcription; Histology; Human; Image; Immunohistochemistry; In Situ; Individual; Label; Location; Malignant Neoplasms; Maps; Messenger RNA; Methods; Molecular; Morphology; Nucleic Acids; Operating System; Organ; Paraffin Embedding; Pattern; Peptide Signal Sequences; Phase; Protein Analysis; Proteins; Proteome; Publishing; RNA; Regulation; Research Personnel; Resolution; Sampling; Scheme; Signal Transduction; Site; Specificity; System; Techniques; Technology; Testing; Therapeutic; Time; Tissue Microarray; Tissue Sample; Tissues; Translating; Work; antibody conjugate; biological systems; brain tissue; cell type; complex biological systems; design; fluorescence imaging; high throughput technology; insight; laser capture microdissection; new technology; novel; prognostic; protein expression; public health relevance

SUMMARY New technologies are needed to enable the multiplexed analysis of proteins. Our aim is to develop a highly parallel assay technology for obtaining information on protein abundance. This would enable the generation of high-resolution maps of the proteome of complex tissues, and assist in the detection of protein interactions. The technology is designed for analyzing complex and dynamic biological systems, including regulatory and communication systems that operate at the cellular and multicellular level.

摘要 需要新的技术来实现蛋白质的多路分析。我们的目标是开发一种高度 用于获取蛋白质丰度信息的并行分析技术。这将使得能够生成 高分辨率的复杂组织蛋白质组图谱，并协助检测蛋白质相互作用。 这项技术旨在分析复杂和动态的生物系统，包括监管和 在蜂窝和多蜂窝级别运行的通信系统。