Multiplexed Nucleic Acid Assays

描述（由申请人提供）：需要新技术来并行地对许多样品中的基因表达进行多重分析。这将使绘制哺乳动物大脑以及其他复杂组织的高分辨率基因表达图谱成为可能。本提案旨在开发新技术以实现此类应用。它适用于分析复杂和动态的生物系统，包括在细胞和多细胞水平上运作的调节和通信系统。

DESCRIPTION (provided by applicant): New technologies are needed to enable the multiplexed analysis of gene expression in many samples in parallel. This would enable the generation of high-resolution maps of gene expression of the mammalian brain, as well as other complex tissues. This proposal aims to develop novel technology to enable applications of this type. It is suited to analyzing complex and dynamic biological systems, including regulatory and communication systems that operate at the cellular and multicellular level. PUBLIC HEALTH RELEVANCE: We are developing technology to enable a deeper understanding of how the genetic blueprint is used to make and regulate the mammalian brain and other complex tissues. The ultimate aim is to provide technology that enables new insights into how complex biological systems work, and how this is altered in disease. This understanding can be translated into diagnostic and prognostic assays, preventative strategies, and therapeutics.