Multiplexed Nucleic Acid Assays

多重核酸检测

• 批准号: 8058889
• 负责人: MARK S. CHEE
• 金额: $ 32.41万
• 依托单位: PROGNOSYS BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8058889
• 关键词: Address; Biochemistry; Biological Assay; Biological Models; Brain; Cells; Communication; Complex; Computer software; DNA; Detection; Device or Instrument Development; Diagnostic; Disease; Formalin; Gene Expression; Gene Expression Regulation; Generations; Genes; Genetic; Gold; In Situ; In Situ Hybridization; Location; Maps; Methods; Molecular; Nucleic Acids; Operating System; Paraffin Embedding; Pattern; Phase; Preparation; Protocols documentation; RNA; Reagent; Regulation; Resolution; Sampling; Signal Transduction; Site; System; Technology; Therapeutic; Tissues; Translating; Work; biological systems; complex biological systems; design; genome-wide; innovation; insight; instrumentation; laser capture microdissection; new technology; next generation; novel; prognostic; prototype

DESCRIPTION (provided by applicant): New technologies are needed to enable the multiplexed analysis of gene expression in many samples in parallel. This would enable the generation of high-resolution maps of gene expression of the mammalian brain, as well as other complex tissues. This proposal aims to develop novel technology to enable applications of this type. It is suited to analyzing complex and dynamic biological systems, including regulatory and communication systems that operate at the cellular and multicellular level. PUBLIC HEALTH RELEVANCE: We are developing technology to enable a deeper understanding of how the genetic blueprint is used to make and regulate the mammalian brain and other complex tissues. The ultimate aim is to provide technology that enables new insights into how complex biological systems work, and how this is altered in disease. This understanding can be translated into diagnostic and prognostic assays, preventative strategies, and therapeutics.

描述（由申请人提供）：需要新技术来实现对许多样品中的基因表达进行并行的多重分析。这将能够生成哺乳动物大脑以及其他复杂组织的高分辨率基因表达图。该提案旨在开发新技术，以实现此类应用。它适用于分析复杂和动态的生物系统，包括在细胞和多细胞水平上运行的调节和通信系统。 公共卫生相关性：我们正在开发技术，以便更深入地了解遗传蓝图如何用于制造和调节哺乳动物大脑和其他复杂组织。最终目标是提供技术，使人们能够对复杂的生物系统如何工作以及如何在疾病中改变有新的见解。这种理解可以转化为诊断和预后测定，预防策略和治疗。