Multiplexed Nucleic Acid Assays

描述(由申请人提供)：需要新的技术来实现对多个样本中的基因表达的多路并行分析。这将使生成哺乳动物大脑以及其他复杂组织的高分辨率基因表达图成为可能。这项提议旨在开发新技术，使这类应用成为可能。它适合于分析复杂和动态的生物系统，包括在细胞和多细胞水平上运行的调节和通信系统。 与公共健康相关：我们正在开发技术，以使人们能够更深入地了解基因蓝图是如何用于制造和调节哺乳动物的大脑和其他复杂组织的。最终目标是提供技术，使人们能够对复杂的生物系统如何工作，以及这种系统在疾病中是如何改变的有新的见解。这种理解可以转化为诊断和预后分析、预防策略和治疗学。

DESCRIPTION (provided by applicant): New technologies are needed to enable the multiplexed analysis of gene expression in many samples in parallel. This would enable the generation of high-resolution maps of gene expression of the mammalian brain, as well as other complex tissues. This proposal aims to develop novel technology to enable applications of this type. It is suited to analyzing complex and dynamic biological systems, including regulatory and communication systems that operate at the cellular and multicellular level. PUBLIC HEALTH RELEVANCE: We are developing technology to enable a deeper understanding of how the genetic blueprint is used to make and regulate the mammalian brain and other complex tissues. The ultimate aim is to provide technology that enables new insights into how complex biological systems work, and how this is altered in disease. This understanding can be translated into diagnostic and prognostic assays, preventative strategies, and therapeutics.