Development and application of a toolbox of biofilm matrix association modules

生物膜基质关联模块工具箱的开发与应用

• 批准号: 8494554
• 负责人: PAULA I WATNICK
• 金额: $ 20.45万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8494554
• 关键词: Antigen Targeting; Antigens; Bacterial Vaccines; Biomedical Engineering; Bioremediations; Cell surface; Cells; Chitinase; Cholera Toxin Protomer B; Collaborations; Development; Enzymes; Excision; Film; Goals; Gram-Negative Bacteria; Laboratories; Methods; Microbial Biofilms; Peptides; Play; Proteins; Proteomics; Resources; Role; Surface; System; Technology; Vibrio cholerae; Work; cell killing; interest; novel strategies; product development; prototype; research study; tool; vaccine delivery; vaccine development

DESCRIPTION (provided by applicant): Our laboratory recently completed the first comprehensive proteomic analysis of a Gram-negative biofilm matrix. This work demonstrated that a subset of secreted proteins is retained in the biofilm matrix. Three proteins that play a structural role in the biofilm were studied in detail. Interestingly, these proteins were found to have unique distributions in the biofilm matrix that correspond to their functions in biofilm formation. The functions and distributions of several more putative biofilm-associated proteins remain to be characterized. We hypothesize that we can harness these biofilm matrix-associated proteins for use in targeting functional enzymes or antigens to specific regions of the biofilm matrix. Here we propose to develop a toolbox of biofilm matrix association modules and then to use this toolbox in proof of principle experiments in which we associate the secreted enzyme chitinase or the B subunit of cholera toxin with the biofilm matrix in specific distributions. Our goal is to establis a novel strategy for association of proteins with the biofilm matrix of Gram-negative bacteria for use in biomedical, biotechnical, and bioengineering applications of Gram-negative bacteria for use in biomedical, biotechnical, and bioengineering applications.

描述（由申请人提供）：我们的实验室最近完成了革兰氏阴性生物膜基质的首次全面蛋白质组学分析。这项工作表明，生物膜基质中保留了一部分分泌蛋白。详细研究了在生物膜中发挥结构作用的三种蛋白质。有趣的是，发现这些蛋白质在生物膜基质中具有独特的分布，与它们在生物膜形成中的功能相对应。几种推定的生物膜相关蛋白的功能和分布仍有待表征。 我们假设我们可以利用这些生物膜基质相关的蛋白来靶向功能性酶或抗原与生物膜基质的特定区域。在这里，我们建议开发一个生物膜基质关联模块的工具箱，然后在原理实验证明我们将分泌的酶几丁质酶或霍乱毒素的B亚基与生物膜基质与特定分布相关联。我们的目标是建立一种新的策略，用于将蛋白质与革兰氏阴性细菌的生物膜基质相关联，用于用于生物医学，生物技术和生物工程细菌的应用，用于生物医学，生物技术，生物技术和生物工程应用。

项目成果

The bacterial biofilm matrix as a platform for protein delivery.

• DOI: 10.1128/mbio.00127-12
• 发表时间: 2012
• 期刊: mBio
• 影响因子: 6.4
• 作者: Absalon C;Ymele-Leki P;Watnick PI
• 通讯作者: Watnick PI