Vibrio cholerae quorum sensing as an intestinal symbiosis factor in a model arthropod host

摘要/项目摘要 霍乱弧菌是一种寄生于节肢动物体内的环境生物，是人类肠道的病原菌 肠子我们的初步结果表明，霍乱弧菌高细胞密度群体感应调节剂， HapR通过以下方式协调这种微生物从肠道病原体到肠道共生体的代谢转变： 延长宿主寿命，激活宿主先天免疫和上皮修复机制，并促进宿主 代谢平衡在这里，我们使用强大的遗传学的模式节肢动物果蝇黑腹 了解宿主和病原体之间的通讯线路，这是这种转变的基础。 我们将鉴定激活宿主先天免疫的细菌代谢物，并追踪激活宿主先天免疫的途径。 细菌为此目的而选择。我们将确定是否节肢动物肠道粘液或 围食膜支持霍乱弧菌的生长，以及HapR活化的降解酶是否消化霍乱弧菌。 酶激活围食膜合成。最后，我们将探讨霍乱弧菌衍生的 色氨酸在促进宿主代谢平衡中的作用。这些研究不仅扩大了我们对 霍乱弧菌HapR在宿主-病原体相互作用中的作用，而且还提供了一种新的群体感应模式 宿主-微生物共生的控制。

Abstract/Project Summary Vibrio cholerae is a human diarrheal pathogen and an environmental organism that persists in the arthropod intestine. Our preliminary results suggest that the V. cholerae high cell density quorum sensing regulator HapR orchestrates the metabolic transition of this microbe from intestinal pathogen to intestinal symbiont by extending host life span, activating host innate immunity and epithelial repair mechanisms, and promoting host metabolic homeostasis. Here we use the powerful genetics of the model arthropod Drosophila melanogaster to understand the lines of communication between host and pathogen that underlie this transition. We will identify bacterial metabolites that activate host innate immunity and trace the host pathways that are co-opted by the bacterium for this purpose. We will determine whether the arthropod intestinal mucus or peritrophic membrane supports growth of V. cholerae and whether digestion by HapR-activated degradative enzymes activates peritrophic membrane synthesis. Finally, we will explore the role of V. cholerae-derived tryptophan in promoting host metabolic homeostasis. These studies not only expand our appreciation of the role of V. cholerae HapR in the host-pathogen interaction but also provide a new paradigm of quorum sensing control of the host-microbe symbiosis.