Cryopreservation Research/Development

低温保存研究/开发

• 批准号: 8654769
• 负责人: DAVID HEIMBROOK
• 金额: $ 4.91万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2018-09-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8654769
• 关键词: Area; Back; Cryopreservation; Development; Disasters; Embryo; Excision; Freezing; Genetic; Genetic Engineering; Genotype; Inbreeding; Laboratories; Liquid substance; Mouse Strains; National Cancer Institute; Nitrogen; Ovary; Services; Staging; Techniques; Vial device; anticancer research; cost effective; cryobiology; mutant; programs; repository; research and development; sperm cell

The Cryopreservation R&D program area offers services for banking unique strains of mice by preserving their frozen germplasm in liquid nitrogen (-196¿ C). Cryopreservation provides a cost-effective alternative to maintaining strains no longer in use by allowing their removal from the shelf storage. Additionally, the laboratory has a storage capacity of 44,000 vials and as a back-up against any imponderable disaster; a number of vials from each strain are kept at the National Cancer Institute-Frederick Central Repository. To further cancer research, the Cryobiology Laboratory utilizes state-of-the-art techniques that make it possible to offer different options for preserving a given mouse strain. Such techniques include freezing embryos at different developmental stages, freezing ovaries, and freezing sperm. With these options we are able to cryopreserve most inbred, spontaneous mutant, or genetically engineered strains, while maintaining the genetic background and genotype.

冷冻保存研发计划领域通过在液氮（-196°C）中保存冷冻种质来为储存独特的小鼠品系提供服务。冷冻保存为维持不再使用的菌株提供了一种经济高效的替代方案，允许将它们从货架上移走。此外，该实验室还拥有 44,000 个小瓶的存储容量，可作为应对任何不可估量的灾难的后备力量；每个菌株的许多小瓶都保存在国家癌症研究所弗雷德里克中央存储库中。为了进一步开展癌症研究，低温生物学实验室利用最先进的技术，为保存特定小鼠品系提供不同的选择。这些技术包括冷冻不同发育阶段的胚胎、冷冻卵巢和冷冻精子。通过这些选项，我们能够冷冻保存大多数近交系、自发突变体或基因工程菌株，同时保持遗传背景和基因型。