Functional analysis of neural crest & palate: Imaging craniofacial development

神经嵴功能分析

• 批准号: 8460776
• 负责人: SCOTT E FRASER
• 金额: --
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-21 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8460776
• 关键词: 3-Dimensional; Affect; Anatomy; Atlases; Birds; California; Cells; Cleft Palate; Cleft lip with or without cleft palate; Collaborations; Communities; Congenital Abnormality; Data; Development; Embryo; Event; Face; Gene Expression; Genes; Goals; Image; Imaging Device; Individual; Laboratories; Life; Light; Link; Live Birth; Magnetic Resonance Imaging; Microscopic; Microscopy; Modeling; Molecular; Morphology; Movement; Mus; Neural Crest; Normal tissue morphology; Palate; Pattern; Phenotype; Preparation; Principal Investigator; Proteins; Quail; Research; Research Personnel; Resources; Role; Signal Pathway; Signal Transduction; Slice; Specialist; Staging; Structure; System; Techniques; Technology; Testing; Therapeutic; Three-Dimensional Image; Three-Dimensional Imaging; Tissues; Transgenic Mice; Transgenic Organisms; Universities; Work; Yang; base; craniofacial; data acquisition; knock-down; meetings; member; molecular imaging; mutant; public health relevance; research study; three dimensional structure; tool

DESCRIPTION (provided by applicant): The research plan will refine and deploy a set of advanced tools for the imaging of tissue structure, gene expression domains and cellular dynamics during craniofacial development. Volumetric imaging tools will be used td create accurate 3D atlases that can be digitally dissected to permit the tissue interactions and cellular events to be better understood in the forming faces of normal, mutant and perturbed mouse and avian embryos. Molecular imaging agents, optimized for imaging intact tissues, will be employed to create atlases of the molecular correlates of these embryos. Finally, intraviral imaging tools will be used to study cell and tissue interactions as they take place, offering a view into the dynamic events that execute craniofacial development. The data will be assembled into atlases that will offer unprecedented tools for exploring the cellular, tissue and molecular correlates of craniofacial development. These atlases will be made widely available for the use of others in the Face Base consortium and the broader community of craniofacial researchers through an online resource created locally. In addition, they will be linked to other Face Base resources through established hubs, so that the data in our atlases can be used in concert with the molecular and structural data provided by other members of the consortium. The data acquisition pipeline established here in close collaboration with the laboratories of Dr. Yang Chai (University of Southern California) and Dr. Marianne Bronner-Fraser (Caltech) will create a model that can be expanded to phenotype and analyze other experimental systems.

描述（由申请人提供）：研究计划将完善和部署一套先进的工具，用于颅面发育过程中的组织结构、基因表达域和细胞动力学成像。将使用体积成像工具来创建精确的3D图谱，这些图谱可以进行数字解剖，以允许在正常、突变和扰动的小鼠和鸟类胚胎的形成面中更好地理解组织相互作用和细胞事件。分子成像剂，优化成像完整的组织，将用于创建这些胚胎的分子相关图谱。最后，病毒内成像工具将用于研究细胞和组织的相互作用，因为它们发生，提供了一个视图到执行颅面发育的动态事件。这些数据将被汇编成地图集，为探索颅面发育的细胞、组织和分子相关性提供前所未有的工具。这些地图集将通过当地创建的在线资源广泛提供给Face Base联盟和更广泛的颅面研究人员社区的其他人使用。此外，它们将通过已建立的中心与其他Face Base资源链接，以便我们地图集中的数据可以与该联盟其他成员提供的分子和结构数据一起使用。与Yang Chai博士（南加州大学）和Marianne Bronner-Fraser博士（加州理工学院）的实验室密切合作建立的数据采集管道将创建一个模型，该模型可以扩展到表型和分析其他实验系统。

项目成果

Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration.

通过 2 光子光片显微镜和回顾性登记捕获活胚胎心脏的动态结构和蛋白质表达。

• DOI: 10.1364/boe.6.002056
• 发表时间: 2015
• 期刊: Biomedical optics express
• 影响因子: 3.4
• 作者: Trivedi,Vikas;Truong,ThaiV;Trinh,LeA;Holland,DanielB;Liebling,Michael;Fraser,ScottE
• 通讯作者: Fraser,ScottE

Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission.

• DOI: 10.1186/s12915-014-0092-2
• 发表时间: 2014-11-11
• 期刊: BMC biology
• 影响因子: 5.4
• 作者: Bower DV;Lee HK;Lansford R;Zinn K;Warburton D;Fraser SE;Jesudason EC
• 通讯作者: Jesudason EC