Functional analysis of neural crest & palate: Imaging craniofacial development

神经嵴功能分析

• 批准号: 8652033
• 负责人: SCOTT E FRASER
• 金额: $ 23.14万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-21 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8652033
• 关键词: Functional; analysis; neural; crest; palate

DESCRIPTION (provided by applicant): The research plan will refine and deploy a set of advanced tools for the imaging of tissue structure, gene expression domains and cellular dynamics during craniofacial development. Volumetric imaging tools will be used td create accurate 3D atlases that can be digitally dissected to permit the tissue interactions and cellular events to be better understood in the forming faces of normal, mutant and perturbed mouse and avian embryos. Molecular imaging agents, optimized for imaging intact tissues, will be employed to create atlases of the molecular correlates of these embryos. Finally, intraviral imaging tools will be used to study cell and tissue interactions as they take place, offering a view into the dynamic events that execute craniofacial development. The data will be assembled into atlases that will offer unprecedented tools for exploring the cellular, tissue and molecular correlates of craniofacial development. These atlases will be made widely available for the use of others in the Face Base consortium and the broader community of craniofacial researchers through an online resource created locally. In addition, they will be linked to other Face Base resources through established hubs, so that the data in our atlases can be used in concert with the molecular and structural data provided by other members of the consortium. The data acquisition pipeline established here in close collaboration with the laboratories of Dr. Yang Chai (University of Southern California) and Dr. Marianne Bronner-Fraser (Caltech) will create a model that can be expanded to phenotype and analyze other experimental systems. PUBLIC HEALTH RELEVANCE: Cleft palate, with or without cleft lip. is one of the more common birth defects, affecting approximately one in 2500 live births. The basic events by which cells of different origin come together to form the face have long been known, but the exact tissue movements and the signals that guide them remain undefined. The goal of this work is to help fill this void by imaging the morphology of normal craniofacial development, the abnormal development seen in cleft palate, and providing a means to experimentally test the roles of signaling pathways in the tissue interactions that build the face. This work offers a powerful platform for devising and testing potential therapeutic approaches.

描述(申请人提供)：该研究计划将完善和部署一套先进的工具，用于在颅面发育过程中对组织结构、基因表达结构域和细胞动力学进行成像。将使用体积成像工具创建精确的3D地图集，这些地图集可以数字解剖，以便更好地了解正常、突变和受扰的小鼠和鸟类胚胎的形成面上的组织相互作用和细胞事件。针对完整组织进行成像优化的分子成像剂将被用来创建这些胚胎的分子相关图谱。最后，病毒内成像工具将用于研究细胞和组织发生的相互作用，提供执行颅面发育的动态事件的视图。这些数据将被汇编成地图集，为探索颅面发育的细胞、组织和分子相关性提供前所未有的工具。这些地图集将通过当地创建的在线资源广泛提供给Face Base联盟的其他人和更广泛的头面部研究人员社区使用。此外，它们将通过建立的枢纽与其他Face Base资源相链接，以便我们地图集中的数据可以与该联盟其他成员提供的分子和结构数据一起使用。这里与杨柴博士(南加州大学)和Marianne Bronner-Fraser博士(加州理工大学)的实验室密切合作建立的数据采集管道将创建一个可扩展到表型和分析其他实验系统的模型。 与公共卫生相关：腭裂，有或没有唇裂。是比较常见的出生缺陷之一，大约每2500名活产儿中就有一名受到影响。不同来源的细胞聚集在一起形成面部的基本事件早已为人所知，但确切的组织运动和引导它们的信号仍然不确定。这项工作的目标是通过成像正常颅面发育的形态，即腭裂中的异常发育，来帮助填补这一空白，并提供一种手段，以实验测试信号通路在构建面部的组织相互作用中的作用。这项工作为设计和测试潜在的治疗方法提供了一个强大的平台。