Molecular and functional characterization of olfaction genes in tsetse flies

采采蝇嗅觉基因的分子和功能特征

基本信息

  • 批准号:
    8411357
  • 负责人:
  • 金额:
    $ 5.67万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-03-20 至 2016-02-29
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The overall goal of this study is to provide information on molecular processes in tsetse vectors that mediate their olfactory processes, enabling them to locate their hosts and other critical resources for their survival and reproduction. The goals will be achieved by prospecting for and validating olfaction responsive genes in selected tsetse species (Glossina pallidipes, Glossina austeni and Glossina fuscipes fuscipes) of medical and veterinary importance in Kenya. We propose an integrated and interdisciplinary approach to: 1) Determine differences in olfactory responsive genes in sympatric Glossina pallidipes and Glossina austeni in Shimba hills and Glossina fuscipes fuscipes in Mbita in Kenya and 2) Determine functional roles of olfactory responsive genes in sympatric G. pallidipes and G. austeni in Shimba hills and G. f. fuscipes in Mbita districts in Kenya. We hypothesize that putative olfactory genes among these flies are orthologuous, differentially expressed and significantly influence responses of the flies to different odors. Adults of the selected tsetse species will be sampled from respective sites and RNA will be extracted from head tissues. We will undertake a discovery project by using next generation sequencing technologies to determine global transcriptome-level heterogeneities exhibited between the different species. The DNA obtained from the gut contents of flies will be subjected to PCR amplification with COI marker to determine the putative host species from Barcode of Life Data systems (BOLD) database. Following the comparative transcriptome analysis from the different species we will in vitro validate the presence and expression profiles of select set of putative olfaction related genes through qPCR. The functional role(s) of selected putative olfaction genes will be further investigated through RNAi gene silencing methodologies in conjunction with electro-antennogram and Wind-Tunnel techniques to establish the roles of these genes in modulating the physiology and behavior of the flies. Availability of comprehensive information on olfaction related genes, and their respective functional roles in modulating tsetse olfactory behavior will facilitate further R&D to exploit these genes to control tsetse fly vectors. This research will be done primarily in Kenya at International Centre of Insect Physiology Ecology and Kenya Agricultural Research Institute - Trypanosomiasis Research Center in collaboration with Paul Mireji with companion grant being Global Infectious Diseases (GID) D43 TW007391 (08/01/2008- 03/31/2013).
描述(由申请人提供): 本研究的总体目标是提供有关舌蝇媒介介导其嗅觉过程的分子过程的信息,使它们能够定位其宿主和其他对其生存和繁殖至关重要的资源。这些目标将通过在肯尼亚具有医学和兽医重要性的选定采采蝇物种(Glossina pallidipes、Glossina pallidipes和Glossina fuscipes fuscipes)中寻找和验证嗅觉反应基因来实现。我们提出了一个综合的和跨学科的方法:1)确定嗅觉反应基因的差异,在同域Glossina pallidipes和Glossina pallidipes在辛巴山和Glossina fuscipes在Mbita在肯尼亚和2)确定嗅觉反应基因的功能作用,在同域G。pallidipes和G. Shimba hills和G. F.在肯尼亚的姆比塔地区。我们假设这些果蝇中的嗅觉基因是同源的,差异表达的,并显着影响果蝇对不同气味的反应。将从各个部位对选定采采蝇种属的成虫进行采样,并从头部组织中提取RNA。我们将通过使用下一代测序技术来确定不同物种之间表现出的全球转录组水平的异质性来进行发现项目。从苍蝇的肠道内容物中获得的DNA将进行具有COI标记的PCR扩增,以从生命数据系统条形码(BOLD)数据库中确定推定的宿主物种。在来自不同物种的比较转录组分析之后,我们将通过qPCR在体外验证所选择的一组推定的嗅觉相关基因的存在和表达谱。通过RNAi基因沉默方法结合触角电位图和风洞技术,将进一步研究选定的推定嗅觉基因的功能作用,以确定这些基因在调节果蝇生理和行为中的作用。嗅觉相关基因及其在调节采采蝇嗅觉行为中的功能作用的全面信息的可用性将有助于进一步研发利用这些基因来控制采采蝇媒介。这项研究将主要在肯尼亚的国际昆虫中心进行。 生理生态学和肯尼亚农业研究所-锥虫病研究中心与保罗Mireji合作,伴随赠款是全球传染病(GID)D43 TW 007391(08/01/2008- 03/31/2013)。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Serap AKSOY其他文献

Serap AKSOY的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Serap AKSOY', 18)}}的其他基金

Support for Vector Biology Training for Sustainable Control of Vector Borne diseases in East Africa
支持媒介生物学培训以可持续控制东非媒介传播疾病
  • 批准号:
    10675897
  • 财政年份:
    2023
  • 资助金额:
    $ 5.67万
  • 项目类别:
Trypanosome Transmission Biology in Tsetse
采采蝇中的锥虫传播生物学
  • 批准号:
    10542426
  • 财政年份:
    2021
  • 资助金额:
    $ 5.67万
  • 项目类别:
Trypanosome Transmission Biology in Tsetse
采采蝇中的锥虫传播生物学
  • 批准号:
    10365155
  • 财政年份:
    2021
  • 资助金额:
    $ 5.67万
  • 项目类别:
Spiroplasma effects on Tsetse Flies
螺原体对采采蝇的影响
  • 批准号:
    10435557
  • 财政年份:
    2021
  • 资助金额:
    $ 5.67万
  • 项目类别:
Spiroplasma effects on Tsetse Flies
螺原体对采采蝇的影响
  • 批准号:
    10287933
  • 财政年份:
    2021
  • 资助金额:
    $ 5.67万
  • 项目类别:
Molecular Aspects of Tsetse and Trypanosome Transmission
采采蝇和锥虫传播的分子方面
  • 批准号:
    10078239
  • 财政年份:
    2019
  • 资助金额:
    $ 5.67万
  • 项目类别:
Molecular Aspects of Tsetse and Trypanosome Transmission
采采蝇和锥虫传播的分子方面
  • 批准号:
    10297859
  • 财政年份:
    2019
  • 资助金额:
    $ 5.67万
  • 项目类别:
2015 Tropical Infectious Diseases Gordon Research Conference & Seminar
2015年热带传染病戈登研究会议
  • 批准号:
    8835890
  • 财政年份:
    2015
  • 资助金额:
    $ 5.67万
  • 项目类别:
Control of Tsetse Fly Transmitted Diseases in Kenya
肯尼亚采采蝇传播疾病的控制
  • 批准号:
    8985655
  • 财政年份:
    2015
  • 资助金额:
    $ 5.67万
  • 项目类别:
Expanding the toolbox for tsetse reproductive biology
扩展采采蝇生殖生物学的工具箱
  • 批准号:
    8789330
  • 财政年份:
    2014
  • 资助金额:
    $ 5.67万
  • 项目类别:

相似海外基金

Co-designing a lifestyle, stop-vaping intervention for ex-smoking, adult vapers (CLOVER study)
为戒烟的成年电子烟使用者共同设计生活方式、戒烟干预措施(CLOVER 研究)
  • 批准号:
    MR/Z503605/1
  • 财政年份:
    2024
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Research Grant
Early Life Antecedents Predicting Adult Daily Affective Reactivity to Stress
早期生活经历预测成人对压力的日常情感反应
  • 批准号:
    2336167
  • 财政年份:
    2024
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Standard Grant
RAPID: Affective Mechanisms of Adjustment in Diverse Emerging Adult Student Communities Before, During, and Beyond the COVID-19 Pandemic
RAPID:COVID-19 大流行之前、期间和之后不同新兴成人学生社区的情感调整机制
  • 批准号:
    2402691
  • 财政年份:
    2024
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Standard Grant
Elucidation of Adult Newt Cells Regulating the ZRS enhancer during Limb Regeneration
阐明成体蝾螈细胞在肢体再生过程中调节 ZRS 增强子
  • 批准号:
    24K12150
  • 财政年份:
    2024
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Migrant Youth and the Sociolegal Construction of Child and Adult Categories
流动青年与儿童和成人类别的社会法律建构
  • 批准号:
    2341428
  • 财政年份:
    2024
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Standard Grant
Understanding how platelets mediate new neuron formation in the adult brain
了解血小板如何介导成人大脑中新神经元的形成
  • 批准号:
    DE240100561
  • 财政年份:
    2024
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Discovery Early Career Researcher Award
Laboratory testing and development of a new adult ankle splint
新型成人踝关节夹板的实验室测试和开发
  • 批准号:
    10065645
  • 财政年份:
    2023
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Collaborative R&D
Usefulness of a question prompt sheet for onco-fertility in adolescent and young adult patients under 25 years old.
问题提示表对于 25 岁以下青少年和年轻成年患者的肿瘤生育力的有用性。
  • 批准号:
    23K09542
  • 财政年份:
    2023
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification of new specific molecules associated with right ventricular dysfunction in adult patients with congenital heart disease
鉴定与成年先天性心脏病患者右心室功能障碍相关的新特异性分子
  • 批准号:
    23K07552
  • 财政年份:
    2023
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Issue identifications and model developments in transitional care for patients with adult congenital heart disease.
成人先天性心脏病患者过渡护理的问题识别和模型开发。
  • 批准号:
    23K07559
  • 财政年份:
    2023
  • 资助金额:
    $ 5.67万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了