Expanding the toolbox for tsetse reproductive biology

扩展采采蝇生殖生物学的工具箱

• 批准号: 8789330
• 负责人: Serap AKSOY
• 金额: $ 18.58万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-06 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8789330
• 关键词: Affect; Africa South of the Sahara; African Trypanosomiasis; Animal Model; Animals; Behavior; Behavioral; Biology; Complex; Computer Simulation; Data; Data Set; Deposition; Development; Disease; Disease Vectors; Disease model; Evolution; Female; Fertility; Fluorescent in Situ Hybridization; Foundations; Future; Gene Expression; Gene Expression Profile; Gene Proteins; Gene Silencing; Genome; Genomic approach; Genomics; Gland; Goals; Health; Human; Insecta; Knowledge; Longevity; Mediating; Methods; Molecular Genetics; Molecular Profiling; Nucleic Acid Regulatory Sequences; Oogenesis; Organism; Orthologous Gene; Partner in relationship; Pattern; Pesticides; Physiological; Physiology; Play; Polymorphism Analysis; Population; Population Sizes; Proteins; Proteomics; Protocols documentation; Regulation; Regulatory Element; Reproduction; Reproductive Biology; Reproductive Physiology; Research; Resources; Role; Sampling; Seminal fluid; Set protein; Specificity; Spermatogonia; Study models; Synteny; Techniques; Tissues; Toxic effect; Trypanosomiasis; Tsetse Flies; base; comparative; cost; disease transmission; egg; fitness; fly; knowledge base; male; neglect; non-genetic; novel; offspring; paralogous gene; prevent; protein function; reproductive; sex; sperm cell; tool; transcriptomics; transmission process; vector control; young adult

DESCRIPTION (provided by applicant): Tsetse flies transmit the human and animal forms of African Trypanosomiasis, neglected diseases, which affect marginalized populations in sub-Saharan Africa. Control methods based upon pesticide-baited targets and traps can reduce tsetse populations, but have downsides, including environmental toxicity, collateral effects upon non- target organisms and high implementation cost. Thus, genetic and non-pesticide based vector control methods are desirable. This project will build the foundation for control methods that aim to reduce tsetse's reproductive capacity. We will investigate male accessory gland (MAGs) products, known as seminal fluid proteins (SFPs), which are transferred along with sperm to the female during mating. SFPs mediate important physiological and behavioral changes in mated female insects, including reduced receptivity to remating and increased oogenesis/egg deposition. Disruption/manipulation of SFP function(s) has the potential to reduce tsetse populations and hence disease transmission. Tsetse flies have a low reproductive capacity as they reproduce by obligate viviparity. Each gonotrophic cycle, females carry and nourish a single intrauterine offspring for the duration of its development. Females on average produce 8-10 offspring per lifetime. Our proposed studies are supported by the availability of 1) preliminary transcriptomic data on MAG gene expression in our target species Glossina morsitans morsitans (Gmm), 2) an annotated genome and extensive transcriptomic resources for Gmm, and 3) a large dataset of transcriptomes from other tsetse species. This study has 3 aims: 1. Identify putative SFP genes using transcriptomic, proteomic and genomic approaches, characterize their sex/tissue specificity, and examine temporal expression profiles during maturation and after mating. 2. Identify SFP orthologs and paralogs between multiple Glossina species by utilizing available transcriptomic and genomic resources, estimate sequence diversity of SFPs by polymorphism detection and identify conserved sex and tissue specific regulatory elements by in silico analysis. 3. Develop and apply gene silencing techniques to assess the functional role(s) of selected SFPs (~5) within the context of the tsetse reproductive fitness parameters.

描述（由申请人提供）：采采蝇传播人类和动物形式的非洲锥虫病，这是一种被忽视的疾病，影响撒哈拉以南非洲的边缘人群。基于农药诱饵目标和诱捕器的控制方法可以减少采采蝇种群，但也有缺点，包括环境毒性、对非目标生物的附带影响以及实施成本高。因此，基于遗传和非农药的病媒控制方法是可取的。该项目将为旨在降低采采蝇繁殖能力的控制方法奠定基础。我们将研究雄性附属腺 (MAG) 产物，称为精液蛋白 (SFP)，它们在交配过程中与精子一起转移到雌性体内。 SFP 介导交配雌性昆虫的重要生理和行为变化，包括降低重新交配的接受性和增加卵子发生/卵沉积。破坏/操纵 SFP 功能有可能减少采采蝇种群，从而减少疾病传播。采采蝇的繁殖能力较低，因为它们通过专性胎生繁殖。在每个促性腺营养周期中，雌性在其发育期间携带并滋养一个子宫内后代。女性一生平均生育 8-10 个后代。我们提出的研究得到了以下方面的支持：1) 我们的目标物种 Glossina morsitans morsitans (Gmm) 中 MAG 基因表达的初步转录组数据，2) Gmm 的注释基因组和广泛的转录组资源，以及 3) 来自其他采采蝇物种的转录组的大型数据集。本研究有 3 个目标： 1. 使用转录组学、蛋白质组学和基因组学方法鉴定假定的 SFP 基因，表征其性别/组织特异性，并检查成熟期间和交配后的时间表达谱。 2. 利用现有的转录组和基因组资源鉴定多个舌藻物种之间的 SFP 直向同源物和旁系同源物，通过多态性检测估计 SFP 的序列多样性，并通过计算机分析鉴定保守的性别和组织特异性调控元件。 3. 开发并应用基因沉默技术，以评估选定的 SFP (~5) 在采采蝇繁殖适应性参数背景下的功能作用。

项目成果

Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans.

• DOI: 10.1186/s12866-018-1289-2
• 发表时间: 2018-11-23
• 期刊: BMC microbiology
• 影响因子: 4.2
• 作者: Scolari F;Attardo GM;Aksoy E;Weiss B;Savini G;Takac P;Abd-Alla A;Parker AG;Aksoy S;Malacrida AR
• 通讯作者: Malacrida AR