Tissue-dynamics Imaging for Therapeutic Efficacy in Ovarian Cancer

组织动力学成像对卵巢癌治疗效果的影响

• 批准号: 8471381
• 负责人: Daniela E Matei
• 金额: $ 33.96万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8471381
• 关键词: Accounting; Ascites; Asses; Bedside Testings; Biological; Biological Assay; Biological Markers; Biological Sciences; Cancer Biology; Cancer Center; Cancer cell line; Cell Line; Cell Proliferation; Cell physiology; Cells; Cellular Spheroids; Cessation of life; Clinic; Clinical; Clinical Trials; Complex; Development; Diagnosis; Disease; Drug resistance; Epithelial ovarian cancer; Fatal Outcome; Fingerprint; Functional Imaging; Future; Goals; Heterogeneity; Holography; Human; Hypoxia; Image; Imaging technology; Implant; In Vitro; Lighting; Malignant Female Reproductive System Neoplasm; Malignant Neoplasms; Malignant neoplasm of ovary; Measurement; Measures; Metastatic to; Modeling; Monitor; Motion; Neoplasm Metastasis; Operative Surgical Procedures; Optics; Ovarian; Patients; Peritoneal; Pharmaceutical Preparations; Physics; Platinum; Primary Neoplasm; Process; Property; Recurrence; Relative (related person); Resistance; Science; Solid Neoplasm; Source; Specimen; Staging; Stratification; System; Taxane Compound; Technology; Technology Transfer; Testing; Therapeutic; Therapeutic Agents; Tissues; Translating; Treatment Efficacy; Treatment Protocols; Tumor-Derived; United States; Woman; Work; Xenograft procedure; base; cancer care; cancer cell; cancer therapy; chemotherapy; clinical application; clinically relevant; design; digital; drug efficacy; drug mechanism; experience; improved; in vitro Model; innovation; intraperitoneal; light scattering; malignant ascites; millimeter; new technology; novel; ovarian neoplasm; physical science; public health relevance; research study; response; response marker; screening; taxane; tumor; tumor microenvironment

DESCRIPTION (provided by applicant): This project will employ a non-traditional imaging technology called tissue-dynamics imaging (TDI) to measure the sensitivity of ovarian tumors to chemotherapy and biological treatments. Tissue-dynamics imaging is the first imaging technology to use intracellular motion as an internal vital-signs monitor of cancer tissue. TDI uses digital holography with low-coherence illumination to construct 3D holograms up to a millimeter inside tissue. Dynamic imaging detects diverse cellular motions as endogenous image contrast in a functional imaging approach. Three-dimensional solid tumor models, such as tumor exgrafts and multicellular tumor spheroids, are ideal in vitro models to study complex three-dimensional tumor microenvironments, tumor heterogeneity, and multicellular drug resistance. Tissue dynamics imaging provides the required depth capability, the sensitivity to cellular motions, and the signatures of different dynamical cellular functions to provide quantitative measurements of cellular functioning and proliferation. In the proposed work, tissue dynamics imaging will be used to generate the first drug-response phenotypic profiles in cancer biology applied to tumor exgrafts. The goal is to design a reproducible and sensitive predictive marker to therapy. Current approaches that measure chemo-resistance are cumbersome and not predictive of biological and clinical response. By bridging between the fields of cancer biology and coherent optical physics, we propose that by exploiting the intracellular dynamical properties of ovarian tumors or metastatic implants ex-vivo, this new technology can be adapted to overcome a problem of high clinical relevance for women with ovarian cancer. A commercial partner, Animated Dynamics LLC, will receive technology transfer and construct the first clinic-based TDI system.

描述（由申请人提供）：该项目将采用一种称为组织动力学成像（TDI）的非传统成像技术来测量卵巢肿瘤对化疗和生物治疗的敏感性。组织动力学成像是第一个使用细胞内运动作为癌组织内部生命体征监测器的成像技术。TDI使用低相干照明的数字全息术在组织内部构建高达一毫米的3D全息图。动态成像检测不同的细胞运动作为功能成像方法中的内源性图像对比度。三维实体瘤模型，如肿瘤外植物和多细胞肿瘤球体，是研究复杂三维肿瘤微环境、肿瘤异质性和多细胞耐药的理想体外模型。组织动力学成像提供了所需的深度能力、对细胞运动的灵敏度以及不同动态细胞功能的特征，以提供细胞功能和增殖的定量测量。在拟议的工作中，组织动力学成像将用于产生第一个药物反应表型分布在癌症生物学应用于肿瘤移植物。我们的目标是设计一个可重复的和敏感的预测标记治疗。目前测量化学抗性的方法是繁琐的，并且不能预测生物学和临床反应。通过癌症生物学和相干光学物理学领域之间的桥梁，我们提出，通过利用卵巢肿瘤或转移性植入物的细胞内动力学特性，这种新技术可以适用于克服卵巢癌妇女的高度临床相关性的问题。一个商业合作伙伴，动画动力有限责任公司，将接受技术转让，并建立第一个基于临床的TDI系统。