Center for Chromatin NanoImaging in Cancer

癌症染色质纳米成像中心

• 批准号: 10830067
• 负责人: Daniela E Matei
• 金额: $ 16.93万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-12-10 至 2026-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10830067
• 关键词: Cancer cell line; Cells; Chemoresistance; Chromatin; DNA; DNA Binding; DNA analysis; Fluorescence; Fluorescent Dyes; Goals; Histones; Human; Image; Investigation; Label; Malignant Neoplasms; Malignant neoplasm of ovary; Neoplasm Metastasis; Proteins; Resistance; Stains; Universities; biological research; cancer cell; cancer stem cell; chemotherapy; fluorophore; multimodality; nano; nanoimaging; small molecule; stemness

One of the major goals for the Northwestern University U54 team is to understand alterations in chromatin organizations using multimodality nanoscopic imaging and explore a potential correlation between imaged chromatin spatial organizations with the stemness of cancers and the mechanism of chemotherapy resistance. The team has used human-derived and ovarian cancer cells and commercial ovarian cancer cell lines for the proposed investigation. In this project, the team proposes to develop photoswitchable DNA labels by modifying commonly used small-molecule DNA binding fluorescent dyes, such as Hoechst or 4′,6-diamidino-2-phenylindole (DAPI), that are already widely used fluorophores for staining DNA in biological research. Using the new photoswitchable DNA label, the team will simultaneously label both DNA and histone proteins in control and ovarian cancer cells to achieve multiplexed sSMLM imaging. The team will also achieve nanodomain spatial contact analyses of DNA and histone proteins and correlate with ovarian cancer stem cells and cells resistant to chemotherapy.

西北大学U54团队的主要目标之一是了解染色质的改变 使用多模式的组织纳米镜成像并探索成像之间的潜在相关性 具有癌症干性和化学疗法抗性机制的编年素空间组织。 该团队已使用人类衍生和卵巢癌细胞以及商业卵巢癌细胞系 拟议的投资。在这个项目中，团队的提议通过修改来开发可开关的DNA标签 常用的小分子DNA结合荧光染料，例如Hoechst或4'，6-Diamidino-2-苯基吲哚 （DAPI），在生物学研究中已经广泛使用用于染色DNA的荧光团。使用新的 该团队将仅将DNA和Hisstone蛋白贴上可控的DNA标签，并将其标记为 卵巢癌细胞实现多重SSMLM成像。团队还将实现纳米域空间 DNA和组蛋白的接触分析，并与卵巢癌干细胞和抗性细胞相关 化学疗法。