Center for Chromatin NanoImaging in Cancer

癌症染色质纳米成像中心

• 批准号: 10830067
• 负责人: Daniela E Matei
• 金额: $ 16.93万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-12-10 至 2026-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10830067
• 关键词: Cancer cell line; Cells; Chemoresistance; Chromatin; DNA; DNA Binding; DNA analysis; Fluorescence; Fluorescent Dyes; Goals; Histones; Human; Image; Investigation; Label; Malignant Neoplasms; Malignant neoplasm of ovary; Neoplasm Metastasis; Proteins; Resistance; Stains; Universities; biological research; cancer cell; cancer stem cell; chemotherapy; fluorophore; multimodality; nano; nanoimaging; small molecule; stemness

One of the major goals for the Northwestern University U54 team is to understand alterations in chromatin organizations using multimodality nanoscopic imaging and explore a potential correlation between imaged chromatin spatial organizations with the stemness of cancers and the mechanism of chemotherapy resistance. The team has used human-derived and ovarian cancer cells and commercial ovarian cancer cell lines for the proposed investigation. In this project, the team proposes to develop photoswitchable DNA labels by modifying commonly used small-molecule DNA binding fluorescent dyes, such as Hoechst or 4′,6-diamidino-2-phenylindole (DAPI), that are already widely used fluorophores for staining DNA in biological research. Using the new photoswitchable DNA label, the team will simultaneously label both DNA and histone proteins in control and ovarian cancer cells to achieve multiplexed sSMLM imaging. The team will also achieve nanodomain spatial contact analyses of DNA and histone proteins and correlate with ovarian cancer stem cells and cells resistant to chemotherapy.

西北大学U 54团队的主要目标之一是了解染色质的改变 组织使用多模态纳米成像，并探索成像之间的潜在相关性。 染色质空间结构与肿瘤的干性及化疗耐药机制的关系。 该研究小组使用了人类来源的卵巢癌细胞和商业卵巢癌细胞系， 建议调查。在这个项目中，该团队建议通过修改DNA分子来开发光开关DNA标签。 常用的小分子DNA结合荧光染料，如Hoechst或4′，6-二脒基-2-苯基吲哚 （DAPI），它们已经广泛用于生物研究中对DNA进行染色的荧光团。使用新的 光开关DNA标签，该小组将同时标记DNA和组蛋白蛋白的控制， 卵巢癌细胞实现多重sSMLM成像。该团队还将实现纳米域空间 DNA和组蛋白的接触分析，并与卵巢癌干细胞和耐药细胞相关。 化疗