Tissue-dynamics Imaging for Therapeutic Efficacy in Ovarian Cancer

项目描述（由申请人提供）：本项目将采用一种称为组织动力学成像（TDI）的非传统成像技术来测量卵巢肿瘤对化疗和生物治疗的敏感性。组织动力学成像是第一个使用细胞内运动作为癌症组织内部生命体征监测的成像技术。TDI使用低相干照明的数字全息技术在组织内部构建高达一毫米的3D全息图。动态成像检测不同的细胞运动作为内源性图像对比在功能成像方法。三维实体肿瘤模型，如肿瘤移植物和多细胞肿瘤球体，是研究复杂三维肿瘤微环境、肿瘤异质性和多细胞耐药的理想体外模型。组织动力学成像提供了所需的深度能力，对细胞运动的敏感性，以及不同动态细胞功能的特征，以提供细胞功能和增殖的定量测量。在提出的工作中，组织动力学成像将用于产生癌症生物学中应用于肿瘤移植的第一个药物反应表型谱。目标是设计一种可重复且敏感的治疗预测标志物。目前测量化疗耐药性的方法很繁琐，不能预测生物和临床反应。通过癌症生物学和相干光学物理学领域的桥梁，我们提出，通过利用卵巢肿瘤或转移性植入物的细胞内动力学特性，这项新技术可以适应于克服卵巢癌女性患者的高临床相关性问题。商业合作伙伴动画动力有限责任公司将获得技术转让并构建第一个基于临床的TDI系统。

DESCRIPTION (provided by applicant): This project will employ a non-traditional imaging technology called tissue-dynamics imaging (TDI) to measure the sensitivity of ovarian tumors to chemotherapy and biological treatments. Tissue-dynamics imaging is the first imaging technology to use intracellular motion as an internal vital-signs monitor of cancer tissue. TDI uses digital holography with low-coherence illumination to construct 3D holograms up to a millimeter inside tissue. Dynamic imaging detects diverse cellular motions as endogenous image contrast in a functional imaging approach. Three-dimensional solid tumor models, such as tumor exgrafts and multicellular tumor spheroids, are ideal in vitro models to study complex three-dimensional tumor microenvironments, tumor heterogeneity, and multicellular drug resistance. Tissue dynamics imaging provides the required depth capability, the sensitivity to cellular motions, and the signatures of different dynamical cellular functions to provide quantitative measurements of cellular functioning and proliferation. In the proposed work, tissue dynamics imaging will be used to generate the first drug-response phenotypic profiles in cancer biology applied to tumor exgrafts. The goal is to design a reproducible and sensitive predictive marker to therapy. Current approaches that measure chemo-resistance are cumbersome and not predictive of biological and clinical response. By bridging between the fields of cancer biology and coherent optical physics, we propose that by exploiting the intracellular dynamical properties of ovarian tumors or metastatic implants ex-vivo, this new technology can be adapted to overcome a problem of high clinical relevance for women with ovarian cancer. A commercial partner, Animated Dynamics LLC, will receive technology transfer and construct the first clinic-based TDI system.