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Fabrication of 3D Cardiac Patches for Myocardial Recovery

用于心肌恢复的 3D 心脏贴片的制作

基本信息

批准号：
8453242
负责人：
RAVI Kumar BIRLA
金额：
$ 28.87万
依托单位：
UNIVERSITY OF HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-01 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8453242
关键词：
3-Dimensional Affect Apoptosis Architecture Area Biological Biomedical Engineering Cardiac Cardiac Myocytes Cardiovascular Diseases Cardiovascular system Cause of Death Cell Culture Techniques Cell Density Cell Survival Cells Chronic Cicatrix Clinical Confocal Microscopy Coupled Development Devices Electric Stimulation Environment Exhibits Fibrin Frequencies Gel Gene Expression Profile Growth Factor Heart Heart Transplantation Heart failure Hour Implant Infarction Inflammatory Response Injury Insulin-Like Growth Factor I Intervention Left Left Ventricular Function Life Mechanics Medical Methods Metric Modality Modeling Myocardial Myocardial Infarction Myocardial Ischemia Myocardium Necrosis Organ Donor Patients Performance Perfusion Physiological Property Protocols documentation Publishing Recovery Recovery of Function Research Rodent Model Site Staging Stretching Surface Surgical sutures Technology Testing Therapeutic Therapeutic Intervention Time Tissue Engineering Tissues Transforming Growth Factors Ventricular Work alternative treatment base conditioning density design hemodynamics implantation improved in vivo injured insight neovascularization novel novel strategies novel therapeutics pressure protein expression response scaffold tissue repair

项目摘要

PROJECT SUMMARY/ABSTRACT Cardiovascular disease affects 80 million people in the US and is the leading cause of death. Significant limitations of current treatments necessitate the development of novel strategies. Cardiovascular tissue engineering is an emerging field focused on the development of biological substitutes to restore, maintain, or improve tissue function. Bioengineered 3-dimensional (3D) cardiac patches can be utilized clinically at the site of a myocardial infarction, promoting increased left ventricular contractile function. This study is focused on the fabrication of 3D heart muscle, using controlled stretch to support tissue development and testing the ability of cardiac patches to augment the function of infarcted myocardium. The proposed study is divided into three components. The first part of this study is focused on defining cell culture conditions to maximize the functional performance of 3D cardiac patches. The second part is focused on developing protocols to deliver controlled stretch to the 3D cardiac patches, in order to further increase the functional performance of cardiac patches. In the third part of this project, cardiac patches fabricated using optimized cell culture conditions and conditioned using stretch, will be implanted on the surface of infarcted hearts. The ability of the cardiac patches to augment the functional performance of failing myocardium will be studied, as a function of post-injury time and patch implantation time. Successful completion of this study will result in the development of 3D cardiac patches which will be functional closer to normal mammalian heart muscle and will also provide insight into the potential applicability of the cardiac patches in supporting functional recovery of failing myocardium.

项目摘要/摘要心血管疾病在美国影响着8000万人，是主要的死亡原因。当前治疗方法的重大局限性要求开发新的策略。心血管病组织工程学是一个新兴的领域，专注于开发生物替代品来修复，维持或改善组织功能。可以利用生物工程的3维(3D)心脏贴片临床上在心肌梗死部位，促进左心室收缩功能增强。这研究的重点是制造3D心肌，使用受控拉伸来支持组织发育以及检测心脏补片增强梗死心肌功能的能力。拟议的研究分为三个部分。这项研究的第一部分集中在定义细胞培养条件，以最大限度地发挥3D心脏补片的功能性能。第二部分是重点是开发向3D心脏贴片提供受控拉伸的方案，以便进一步提高心脏补片的功能性能。在这个项目的第三部分，心脏贴片使用优化的细胞培养条件制造并使用STRAND进行调节，将被植入梗塞的心脏表面。心脏补片增强衰竭患者功能表现的能力将研究心肌作为损伤后时间和补片植入时间的函数。这项研究的成功完成将导致3D心脏贴片的开发，这将是功能更接近正常哺乳动物的心肌，也将提供对潜在适用性的洞察心脏补片在支持衰竭心肌功能恢复中的作用。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

16-Channel Flexible System to Measure Electrophysiological Properties of Bioengineered Hearts.

用于测量生物工程心脏电生理特性的 16 通道灵活系统。

DOI：
10.1007/s13239-017-0336-8
发表时间：
2018
期刊：
Cardiovascular engineering and technology
影响因子：
1.8
作者：
Salazar,BetsyH;Hoffman,KristopherA;Reddy,AnilkumarK;Madala,Sridhar;Birla,RaviK
通讯作者：
Birla,RaviK

Optimizing a spontaneously contracting heart tissue patch with rat neonatal cardiac cells on fibrin gel.

在纤维蛋白凝胶上，用大鼠新生儿心脏细胞自发地收缩心脏组织斑块。