15 Tesla Bruker SolariX FT-ICR mass spectrometer

15 Tesla Bruker SolariX FT-ICR 质谱仪

• 批准号: 8335038
• 负责人: RICHARD M CAPRIOLI
• 金额: $ 159.66万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8335038
• 关键词: Accounting; Advisory Committees; Aging; Apoptosis; Basement membrane; Biological; Childhood; Communities; Complex; Development; Diabetic Nephropathy; Engineering; Ensure; Fees; Funding; Glucose; Injury; Kidney; Lesion; MIP gene; Mass Spectrum Analysis; Mediating; Minor; Mycobacterium tuberculosis; Neuraxis; Performance; Post-Translational Protein Processing; Procedures; Proteomics; Reovirus; Research; Research Personnel; Resolution; Role; Sampling; Services; Source; Specialist; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Speed; System; Techniques; Tissue Engineering; United States National Institutes of Health; Universities; chemotherapy; data acquisition; instrument; lens; magnetic field; mass analyzer; mass spectrometer; medical schools; molecular imaging; research study; response; semilunar valve

DESCRIPTION (provided by applicant): We request funds for a 15T Bruker Daltonics solariX FT-ICR mass spectrometer with a dual ESI/MALDI source. The instrument will provide NIH-funded users with critical needs including high mass resolving power, high mass accuracy and high-throughput data acquisition; enhanced sensitivity and dynamic range; high- spatial resolution high-performance molecular imaging; and an expanded mass range in comparison to our current FTMS system and other commercially available high-performance mass analyzers. The purchased instrument will replace our current 9.4T Apex-Qe FT-ICR MS. Major drivers for the choice the 15T solariX system is the significantly increased dynamic range from the higher magnetic field and the overall increase in performance from the instrumental components including the ability to perform high-speed CASI experiments, a technique that can enhance the dynamic range of selected mass windows over 100-fold. The instrument will support a diverse group of major and minor users and the extensively NIH-funded Vanderbilt University research community as a whole. Highlighted NIH funded Major Users are as follows. Dr. Kevin Schey: The Role of Aquaporin-0 in Lens Development and Aging. Dr. Terence S. Dermody: Understanding Cellular Mechanisms Mediating Reovirus-Induced Apoptosis in the Central Nervous System. Dr. Clifton E. Barry, III: Unique Lesion Types and Chemotherapy Response of M. tuberculosis. Dr. Billy G. Hudson: Modification of Proteins in the Basement Membrane and Glomerulus by Glucose in Diabetic Nephropathy. Dr. H. Scott Baldwin: Proteomic Characterization of Semilunar Valve Maturation towards Pediatric Tissue Engineered Valves. Dr. Volker Hans Haase: Understanding the Role of HIF-1 and HIF-2 in the Progression of Renal Injury. The 15T Bruker solariX FT-ICR instrument will be located in the Mass Spectrometry Research Center at Vanderbilt University School of Medicine. The instrument will be managed by a team of 6 professionals including a FT-ICR mass spectrometry specialist and a mass spectrometry engineer. Operational funds will be generated from user fees assessed to investigators for service using standard accounting and billing procedures that have been used by the MSRC services cores for years. Advisory committees are in place to ensure proper use and sustainability of the proposed instrument. The proposed instrument will bring critical and unparalleled new capabilities to the Vanderbilt community providing highly accurate, sensitive and detailed information for highly complex biological samples. PUBLIC HEALTH RELEVANCE: The requested 15T Bruker Daltonics solariX FT-ICR mass spectrometer delivers critical and unparalleled new capabilities in providing high mass accuracy, high sensitivity and much greater dynamic range so as to provide detailed information for highly complex biological samples for the Vanderbilt University community. This high performance instrument is a necessary tool to provide new information to help understand the molecular complexity of biological systems.

描述（由申请人提供）：我们申请一台15T布鲁克道尔顿solariX FT-ICR质谱仪，双ESI/MALDI源。该仪器将为美国国立卫生研究院资助的用户提供关键需求，包括高质量分辨率、高质量精度和高通量数据采集；增强的灵敏度和动态范围；高空间分辨率高性能分子成像；与我们目前的FTMS系统和其他市售的高性能质量分析仪相比，质量范围更大。购买的仪器将取代我们目前的9.4T Apex-Qe FT-ICR ms。选择15T solariX系统的主要驱动因素是高磁场显著增加的动态范围，以及仪器组件性能的整体提高，包括执行高速CASI实验的能力，这项技术可以将所选质量窗口的动态范围提高100倍以上。该仪器将支持不同群体的主要和次要用户以及广泛的美国国立卫生研究院资助的范德比尔特大学研究社区作为一个整体。突出显示的NIH资助的主要用户如下。Kevin Schey博士：水通道蛋白0在晶状体发育和衰老中的作用。Terence S. Dermody博士：了解呼肠孤病毒介导中枢神经系统细胞凋亡的细胞机制。Dr. Clifton E. Barry， III：结核分枝杆菌的独特病变类型和化疗反应。Dr. Billy G. Hudson：糖尿病肾病中葡萄糖对基底膜和肾小球蛋白的修饰。H. Scott Baldwin博士：儿童组织工程瓣膜半月瓣成熟的蛋白质组学特征。Dr. Volker Hans Haase：了解HIF-1和HIF-2在肾损伤进展中的作用。15T布鲁克solariX FT-ICR仪器将位于范德比尔特大学医学院的质谱研究中心。该仪器将由6名专业人员组成的团队管理，包括一名FT-ICR质谱专家和一名质谱工程师。运营资金将从向调查人员收取的服务费中产生，使用标准会计和计费程序，该程序已被MSRC服务核心使用多年。设立了咨询委员会，以确保拟议文书的适当使用和可持续性。该仪器将为范德比尔特社区带来关键的、无与伦比的新能力，为高度复杂的生物样品提供高度准确、敏感和详细的信息。

项目成果

Resolving the Complexity of Spatial Lipidomics Using MALDI TIMS Imaging Mass Spectrometry

• DOI: 10.1021/acs.analchem.0c02520
• 发表时间: 2020-10-06
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Djambazova, Katerina, V;Klein, Dustin R.;Spraggins, Jeffrey M.
• 通讯作者: Spraggins, Jeffrey M.