15 Tesla Bruker SolariX FT-ICR mass spectrometer

描述（由申请人提供）：我们申请资金用于配备双ESI/MALDI源的15 T Bruker Daltonics solariX FT-ICR质谱仪。该仪器将为NIH资助的用户提供关键需求，包括高质量分辨率，高质量准确度和高通量数据采集;增强的灵敏度和动态范围;高空间分辨率高性能分子成像;以及与我们目前的FTMS系统和其他市售高性能质量分析仪相比，扩大的质量范围。购买的仪器将取代我们目前的9.4T Apex-Qe FT-ICR MS。选择15T solariX系统的主要驱动因素是更高磁场的动态范围显着增加，以及仪器组件性能的整体提高，包括执行高速CASI实验的能力，该技术可以将选定质量窗口的动态范围提高100倍以上。该仪器将支持各种各样的主要和次要用户，以及NIH资助的范德比尔特大学研究社区。重点介绍的NIH资助的主要用户如下。Kevin Schey博士：水通道蛋白-0在透镜发育和衰老中的作用。Dr. Terence S. Dermody：了解中枢神经系统中介导呼肠孤病毒诱导的细胞凋亡的细胞机制。克利夫顿博士E.巴里，III：独特的病变类型和M的化疗反应。结核比利G医生。哈德逊：糖尿病肾病中葡萄糖对基底膜和肾小球中蛋白质的修饰。H博士Scott Baldwin：儿童组织工程瓣膜半月瓣成熟的蛋白质组学表征。Volker Hans Haase博士：了解HIF-1和HIF-2在肾损伤进展中的作用。15 T布鲁克solariX FT-ICR仪器将位于范德比尔特大学医学院的质谱研究中心。该仪器将由6名专业人员组成的团队管理，其中包括一名FT-ICR质谱专家和一名质谱工程师。业务资金将来自调查员使用微软资源中心服务核心多年来一直使用的标准会计和计费程序所收取的使用费。设立了咨询委员会，以确保拟议文书的适当使用和可持续性。拟议的仪器将为范德比尔特社区带来关键的和无与伦比的新能力，为高度复杂的生物样品提供高度准确，灵敏和详细的信息。 公共卫生相关性：所要求的15 T Bruker Daltonics solariX FT-ICR质谱仪在提供高质量准确度、高灵敏度和更大动态范围方面提供了至关重要和无与伦比的新功能，从而为范德比尔特大学社区提供高度复杂生物样品的详细信息。这种高性能仪器是提供新信息以帮助理解生物系统分子复杂性的必要工具。

DESCRIPTION (provided by applicant): We request funds for a 15T Bruker Daltonics solariX FT-ICR mass spectrometer with a dual ESI/MALDI source. The instrument will provide NIH-funded users with critical needs including high mass resolving power, high mass accuracy and high-throughput data acquisition; enhanced sensitivity and dynamic range; high- spatial resolution high-performance molecular imaging; and an expanded mass range in comparison to our current FTMS system and other commercially available high-performance mass analyzers. The purchased instrument will replace our current 9.4T Apex-Qe FT-ICR MS. Major drivers for the choice the 15T solariX system is the significantly increased dynamic range from the higher magnetic field and the overall increase in performance from the instrumental components including the ability to perform high-speed CASI experiments, a technique that can enhance the dynamic range of selected mass windows over 100-fold. The instrument will support a diverse group of major and minor users and the extensively NIH-funded Vanderbilt University research community as a whole. Highlighted NIH funded Major Users are as follows. Dr. Kevin Schey: The Role of Aquaporin-0 in Lens Development and Aging. Dr. Terence S. Dermody: Understanding Cellular Mechanisms Mediating Reovirus-Induced Apoptosis in the Central Nervous System. Dr. Clifton E. Barry, III: Unique Lesion Types and Chemotherapy Response of M. tuberculosis. Dr. Billy G. Hudson: Modification of Proteins in the Basement Membrane and Glomerulus by Glucose in Diabetic Nephropathy. Dr. H. Scott Baldwin: Proteomic Characterization of Semilunar Valve Maturation towards Pediatric Tissue Engineered Valves. Dr. Volker Hans Haase: Understanding the Role of HIF-1 and HIF-2 in the Progression of Renal Injury. The 15T Bruker solariX FT-ICR instrument will be located in the Mass Spectrometry Research Center at Vanderbilt University School of Medicine. The instrument will be managed by a team of 6 professionals including a FT-ICR mass spectrometry specialist and a mass spectrometry engineer. Operational funds will be generated from user fees assessed to investigators for service using standard accounting and billing procedures that have been used by the MSRC services cores for years. Advisory committees are in place to ensure proper use and sustainability of the proposed instrument. The proposed instrument will bring critical and unparalleled new capabilities to the Vanderbilt community providing highly accurate, sensitive and detailed information for highly complex biological samples. PUBLIC HEALTH RELEVANCE: The requested 15T Bruker Daltonics solariX FT-ICR mass spectrometer delivers critical and unparalleled new capabilities in providing high mass accuracy, high sensitivity and much greater dynamic range so as to provide detailed information for highly complex biological samples for the Vanderbilt University community. This high performance instrument is a necessary tool to provide new information to help understand the molecular complexity of biological systems.