Cellular Imaging Core

细胞成像核心

基本信息

批准号：
8381880
负责人：
Vittorio Gallo
金额：
$ 21.57万
依托单位：
CHILDREN'S RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8381880
关键词：
3-Dimensional Adopted Adult Affinity Age Amygdaloid structure Animal Model Animals Area Astrocytes Autistic Disorder Binding Biochemical Biological Biology Biometry Blood - brain barrier anatomy Books Brain Cell Culture Techniques Cell Death Cell Line Cell Transplantation Cells Characteristics Child Coculture Techniques Collaborations Computer Interface DNA Data Data Analyses Defect Detection Development Devices Dimensions Direct immunofluorescence Disease Dyes Electron Microscope Electron Microscopy Electroporation Embryo Embryonic Development Equipment Event Fluorescence Fragile X Syndrome Freezing Funding Genetic Materials Genomics Goals Grant Growth Factor Hormones Hour Human Human Resources Image Image Analysis Imaging technology Immigration Immunofluorescence Microscopy In Situ Indirect Immunofluorescence Informatics Institutes Journals Laser Scanning Confocal Microscopy Lasers Life Light Lighting Mediating Mental Retardation and Developmental Disabilities Research Centers Messenger RNA Metabolism Methods Microscope Microscopic Mitotic Molecular Profiling Nature Neuroglia Neurons Neurosciences Neurotransmitter Receptor Oligodendroglia Optics Paraffin Embedding Pathology Patients Phenotype Photons Phototoxicity Population Preparation Procedures Process Proteomics Protocols documentation Publications Publishing Quantum Dots Reagent Reporter Research Research Institute Research Personnel Research Project Grants Resolution Rodent Role Sampling Scanning Scientist Services Signal Pathway Signal Transduction Pathway Site Slice Source Specimen Staging System Staining method Stains Stem cells Synapses System Techniques Thick Time Tissue Sample Tissue Stains Tissues Tracer Transfection Tube Viral Proteins Work Writing animal tissue cell fixing cell motility cellular imaging design detector experience fluorescence imaging gamma-Aminobutyric Acid genetic analysis image registration imaging modality in utero in vivo innovation instrument instrumentation interest knock-down laser capture microdissection light microscopy light scattering medical schools migration mouse model nerve stem cell neuroblast neurotoxic optical disc overexpression photomultiplier postnatal progenitor reconstruction relating to nervous system repaired research study stem tissue culture tissue/cell preparation tool trafficking transmission process

项目摘要

E. Justification A key goal of this Core is to provide access to state-of-the-art imaging technologies to researchers at CN and GWU to perform studies on cells and animal models in this area of IDDR. In addition, the Cellular Imaging and Analysis Core assist investigators in their studies on a variety of children's diseases through the expertise of the staff and the affiliated users. The services offered include assistance in 1) preparation of samples and examination of the cells and tissues by light microscopy and EM (see projects* 1-6), 2) quantitative analysis of the data obtained for each project and 3) obtaining cells and tissue samples to carry out genomics/proteomics analysis (see project#6). For these services we make use of dedicated staff in the Core and also through the communal participation of the users with specific expertise (see section E.2, 3). Additionally, we work together with other Cores including, the Genomics and Proteomics and the Biostatistics and Informatics Cores. Use of the recently acquired laser capture microscope (see section E.6.) has enhanced our ability for targeted extraction of diseased cells and tissues from animal or patient materials further enhancing the analysis of these materials with the help of the Genomics and Proteomics Core. Additionally the services provided for establishing neural cell cultures and cell lines have been valuable for subsequent analysis of these purified populations of cells for quantitative analysis of their molecular profiles through genomic and proteomics analysis. In the coming years, we will increase the support our Core provides in carrying out live imaging and quantitative analysis of imaging data. To this end, we have a new Director with extensive and outstanding experience in both these aspects and have hired a new Core Manager who has background and several years of experience in performing quantitative image analysis. In addition, we have started working closely with the Biostatistics and Informatics Core to enable the users to appropriately design and carry out suitable statistical analysis of their data. We are also working in close collaboration with other IDDRCs nationwide - one example of which is the work that Drs. Corbin and Huntsman's have been performing in collaboration with the IDDRC at the Kennedy Krieger Institute at John's Hopkins Medical School. Their work with Dr. Walter Kaufmann (an expert clinician and scientist in the area of Fragile X Syndrome) makes use of the Cellular Imaging Core and has recently resulted in a publication on inhibitory synaptic defects in a mouse model of Fragile X Syndrome (Olmos-Serano et al., 2010).

E.理由该核心的一个关键目标是向CN和GWU的研究人员提供对最先进的成像技术的访问，以对IDDR该领域的细胞和动物模型进行研究。此外，通过员工和附属用户的专业知识，蜂窝成像和分析核心核心协助研究人员对各种儿童疾病的研究。提供的服务包括在1）通过光学显微镜和EM进行样品制备以及对细胞和组织的检查（请参阅项目* 1-6），2）对每个项目获得的数据的定量分析，以及3）获取细胞和组织样品以进行基因组学/蛋白质组学分析（请参阅项目＃6）。对于这些服务，我们利用核心的专门员工以及用户具有特定专业知识的用户参与（请参阅E.2，3）。此外，我们与其他核心合作，包括基因组学和蛋白质组学以及生物统计学和信息核心。最近获得的激光捕获显微镜的使用（请参见E.6节）增强了我们靶向提取患病细胞和组织中的动物或患者材料组织的能力，进一步增强了这些材料的分析，并借助基因组学和蛋白质组学核心。另外，为建立神经细胞培养和细胞系提供的服务对于随后对这些纯化的分析很有价值通过基因组和蛋白质组学分析，用于对其分子谱的定量分析。在接下来的几年中，我们将增加核心提供的支持，以对成像数据进行实时成像和定量分析。为此，我们拥有一位在这两个方面具有丰富和出色经验的新导演，并聘请了一位新的核心经理，他在执行定量图像分析方面具有背景和数年的经验。此外，我们已经开始与生物统计学和信息学核心紧密合作，以使用户能够适当设计并对其数据进行适当的统计分析。我们还与全国其他IDDRC密切合作，其中一个例子是Dr的作品。 Corbin和Huntsman's一直在John's Hopkins医学院的Kennedy Krieger研究所与IDDRC合作。他们与沃尔特·考夫曼（Walter Kaufmann）博士（脆弱X综合征领域的专家临床医生和科学家）的工作利用了细胞成像核心，最近在脆弱X综合征小鼠模型中发表了有关抑制性突触缺陷的出版物（Olmos-Serano等人（Olmos-Serano等人，2010年，2010年））。