Viral vector Core

病毒载体核心

• 批准号: 8310462
• 负责人: JULIE JOHNSTON
• 金额: $ 8万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8310462
• 关键词: Adenovirus Vector; Adenoviruses; Basic Science; Cloning; Collaborations; Committee Members; Consultations; Control Groups; Country; DNA amplification; Data; Databases; Development; Diabetes Mellitus; Disease; Endocrine; Endocrine System Diseases; Experimental Designs; Gene Delivery; Generations; Goals; Individual; Institution; Laboratories; Lentivirus Vector; Link; Mediating; Mission; Monitor; National Institute of Diabetes and Digestive and Kidney Diseases; Pennsylvania; Prevention; Production; Quality Control; Research; Research Personnel; Resources; Serotyping; Services; Subfamily lentivirinae; Technology; Therapeutic Studies; United States National Institutes of Health; Universities; Validation; Viral Vector; Work; adeno-associated viral vector; base; design; experience; gene therapy; gene transfer vector; improved; innovation; interest; member; novel; preclinical study; prevent; programs; recombinant virus; research study; vector; web based interface

The Viral Vector Core is a critical resource for Penn Diabetes Research Center (DRC) members interested in the use of viral vectors for gene therapy and basic research applications. The Vector core provides adenoviral vectors, adeno-associated viral vectors (AAV) and lentiviral vectors and offers a full range of services including cloning, DNA amplification and characterization, vector design and consultation. The Core is located within one of the premier gene therapy research laboratories in the country and specializes in the production and distribution of novel AAV serotype vectors and pseudotyped lentiviral vectors. The objectives of the DRC Vector Core are as follows: Using state-of-the-art facilities and dedicated professional staff, the Core will: (1) provide DRC Center members with access to advanced vector technologies for use in diabetes and related endocrine disorder applications; (2) work with DRC executive committee members to develop vectors or recombinant viruses specifically for use by DRC members; (3) transition newly created vectors with superior transduction profiles developed in Penn laboratories to the Core for validation and distribution to DRC members; (4) provide consultation to DRC members for vector construction and experimental design and (5) develop a web-based interface to accompany the recently developed Vector Core database to enable DRC members to request services, monitor the status of vector production and access historical data on all vectors produced for their laboratory by the Vector Core. The overall goal of the DRC Vector Core, like that of the larger Penn DRC, is to serve investigators at Penn and surrounding institutions, and aid in the advancement of diabetes-related research toward the goal of prevention, treatment, and cures for this devastating disease.

病毒载体核心是对使用病毒载体进行基因治疗和基础研究应用感兴趣的宾夕法尼亚糖尿病研究中心（DRC）成员的关键资源。Vector core提供腺病毒载体、腺相关病毒载体（AAV）和慢病毒载体，并提供全方位的服务，包括克隆、DNA扩增和表征、载体设计和咨询。该核心位于该国首屈一指的基因治疗研究实验室之一，专门从事新型AAV血清型载体和假型慢病毒载体的生产和分销。DRC Vector Core的目标如下：利用最先进的设施和专业的工作人员，核心将：（1）为DRC中心成员提供用于糖尿病和相关内分泌疾病应用的先进载体技术;（2）与DRC执行委员会成员合作，开发专供DRC成员使用的载体或重组病毒;（3）为DRC成员提供技术支持。（3）将在Penn实验室开发的具有上级转导谱的新创建的载体转移到核心，用于验证和分配给DRC成员;（4）为DRC成员提供矢量构建和实验设计方面的咨询，以及（5）开发一个基于网络的界面，以配合最近开发的矢量核心数据库，使DRC成员能够请求服务，监测病媒生产的状况，并查阅病媒核心为其实验室生产的所有病媒的历史数据。DRC Vector Core的总体目标与较大的Penn DRC一样，是为Penn和周边机构的研究人员提供服务，并帮助推进糖尿病相关研究，以实现预防，治疗和治愈这种毁灭性疾病的目标。