Vector Core

核心A-矢量核心 宾夕法尼亚大学的Vector Core是一个独立的全方位服务实验室， 基因转移领域的试剂和专业知识。这个名为“Penn Vector”的设施由朱莉博士 约翰斯顿，他管理着AAV、腺病毒和慢病毒载体生产领域的8位科学家。.这 该计划将侧重于利用Penn载体生产AAV载体，这些载体将服务于项目I 通过三。由Pranay Khare博士指导的独立质量控制程序执行一系列 释放载体用于体内研究所必需的测定。这个核心也会分析组织 利用TaqMan和PCR技术对载体基因组进行了分析。最后，Vector Core将采用现有的方法 用于大规模生产的临床候选药物的生产和纯化， 支持大型动物研究。该实验室根据GLP的一般原则运作，包括， 文件（批记录表）、SOP、QA/QC、培训和设备验证，尽管它不 代表自己完全符合GLP或GMP。 Lay描述。P01中使用的所有载体将由Vector Core创建和生产。 相关性（参见说明）：

Core A- Vector Core The Vector Core at the University of Pennsylvania is an independent and full service laboratory providing reagents and expertise in the area of gene transfer. This facility called "Penn Vector" is directed by Dr. Julie Johnston who manages 8 scientists in the area of AAV, adenoviral and lentiviral vector production. .This program will focus on utilization of Penn vector for the production of AAV vectors that will service Projects I through III. An independent Quality Control Program directed by Dr. Pranay Khare performs a series of assays necessary for release of the vector for in vivo studies. This core will also analyze tissues for presence of vector genomes using TaqMan and PCR technology. Finally, the Vector Core will adapt existing methods of manufacturing and purification to the clinical candidate for a large scale production efficient enough to support large animal studies. The laboratory operates under the general principles of GLP including, documentation (batch record forms), SOPs, QA/QC, training and equipment validation, although it does not represent itself as being fully GLP or GMP. Lay description. All vectors used in the P01 will be created and produced by the Vector Core. RELEVANCE (Seeinstructions):