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Vector Core

矢量核心

基本信息

批准号：
7595332
负责人：
JULIE JOHNSTON
金额：
$ 20.43万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7595332
关键词：
Abetalipoproteinemia Adenovirus Vector Adenoviruses Animals Archives Area Arts Basic Science Biological Assay Cardiovascular Diseases Clinical Control Groups DNA Viruses Databases Dependovirus Development Documentation Equipment Familial Hypercholesterolemia Gene Delivery Gene Transfer Generations Guidelines Laboratories Lentivirus Vector Link Location Maps Mediating Methodology Methods Molecular Biology Outcome Pennsylvania Principal Investigator Procedures Process Production Quality Control Reagent Regulation Research Research Personnel Resources Scientist Screening procedure Series Serotyping Services Somatic Gene Therapy Subfamily lentivirinae Technology Time Study Tissues Training Universities Validation Viral Vector adeno-associated viral vector base design experience gene therapy good laboratory practice improved in vivo innovation interest large scale production meetings novel preclinical study programs quality assurance repository vector vector genome

项目摘要

Core A- Vector Core The Vector Core at the University of Pennsylvania is an independent and full service laboratory providing reagents and expertise in the area of gene transfer. This facility called "Penn Vector" is directed by Dr. Julie Johnston who manages 8 scientists in the area of AAV, adenoviral and lentiviral vector production. .This program will focus on utilization of Penn vector for the production of AAV vectors that will service Projects I through III. An independent Quality Control Program directed by Dr. Pranay Khare performs a series of assays necessary for release of the vector for in vivo studies. This core will also analyze tissues for presence of vector genomes using TaqMan and PCR technology. Finally, the Vector Core will adapt existing methods of manufacturing and purification to the clinical candidate for a large scale production efficient enough to support large animal studies. The laboratory operates under the general principles of GLP including, documentation (batch record forms), SOPs, QA/QC, training and equipment validation, although it does not represent itself as being fully GLP or GMP. Lay description. All vectors used in the P01 will be created and produced by the Vector Core. RELEVANCE (Seeinstructions):

核心A-矢量核心宾夕法尼亚大学的Vector Core是一个独立的全方位服务实验室，基因转移领域的试剂和专业知识。这个名为“Penn Vector”的设施由朱莉博士约翰斯顿，他管理着AAV、腺病毒和慢病毒载体生产领域的8位科学家。.这该计划将侧重于利用Penn载体生产AAV载体，这些载体将服务于项目I 通过三。由Pranay Khare博士指导的独立质量控制程序执行一系列释放载体用于体内研究所必需的测定。这个核心也会分析组织利用TaqMan和PCR技术对载体基因组进行了分析。最后，Vector Core将采用现有的方法用于大规模生产的临床候选药物的生产和纯化，支持大型动物研究。实验室在GLP的一般原则下运行，包括，文件（批记录表）、SOP、QA/QC、培训和设备验证，尽管它不代表自己完全符合GLP或GMP。 Lay描述。P01中使用的所有载体将由Vector Core创建和生产。相关性（参见说明）：