Nano-Analysis Facility Core

这个共享资源的目的是提供专门的设备和技术，用于使用测序方法来分析基因组中的基因表达、DNA甲基化状态、microRNA签名和基因易位/复制。这些信息将被用来在单细胞水平上更好地理解新的癌症途径，包括允许某些癌细胞进行太阳/化疗的快速细胞进化反应。此共享资源将对当前的排序方法进行扩展 细胞，为每个项目带来高通量测序和其他服务和专业知识。拟议的程序涉及所有步骤的优化，从最终单个细胞的分离和裂解，到整个基因组的扩增和分析。

The purpose ofthis Shared Resource is to provide specialized equipment and techniques for using sequencing methods to analyze gene expression, DNA methylation states, microRNA signatures, and gene translocation/duplication in the genome. This information will be used to better understand novel cancer pathways at the single cell level, including the rapid cell evolution response that allows some cancer cells to sun/ive chemotherapy. This Shared Resource will expand upon current methodology of sequencing a few cells, bringing high throughput sequencing and other services and expertise to each Project. The proposed procedure involves optimization of all steps, from isolation and lysis of, ultimately, a single cell, to whole genome amplification and analysis.