Multifunctional Nanoassemblies for Ligand-directed Imaging and Therapy of Endocri

项目名称：用于内分泌胰腺肿瘤配体定向成像和治疗的多功能纳米组件 项目摘要：针对许多人类疾病，开发用于靶向递送成像探针和治疗剂的工具已成为人们努力的焦点。体内筛选方法可以从工程组合肽库中选择噬菌体靶向特定血管床的能力，该方法发现了一种血管寻址系统，该系统允许对癌症中的血管进行特定的血管生成相关靶向。这种基于噬菌体展示的靶向通过将金和纳米多孔硅纳米粒子直接组装到噬菌体上以用于纳米医学应用而得到扩展。通过利用噬菌体颗粒的纳米尺寸作为分子网络，我们生成了具有生物活性的纳米组件（NA），并具有独特且可调节的化学和物理特性。这些特性包括近红外 (NIR) 辐射转换为热、荧光信号增强、NIR 表面增强拉曼散射 (SERS) 以及结合和合并疗法或成像示踪剂的能力。这种调谐能力与可编程组织靶向相结合，可将多种功能集成到单个 NA 中，并在不同应用（包括化疗靶向和分子成像）中充当补充且非互斥的工具。项目4旨在开发配体导向的Si颗粒-噬菌体-Au颗粒NA作为内分泌胰腺肿瘤靶向成像和治疗的新系统。这些努力将转化为广泛的临床应用。

PROJECT TITLE: Multifunctional Nanoassemblies for Ligand-Directed Imaging and Therapy of Endocrine Pancreatic Tumors PROJECT SUMMARY: The development of tools for the targeted delivery of Imaging-probes and therapeufic agents has become the focus of intense efforts in the context of many human diseases. The in vivo screening method in which phage can be selected from engineered combinatorial peptide libraries for their ability to target specific vascular beds has uncovered a vascular address system that allows specific angiogenesis-related targeting to blood vessels in cancer. This phage display-based targeting Is expanded by the direct-assembly of gold and nanoporous silicon nanoparticles onto phage for nanomedical applications. Through exploiting the nanodimensions of the phage particle as a molecular network we generated biologically active nanoassemblies (NAs) with concomitant unique and tunable chemical and physical properties. These properties include near-infrared (NIR) radiation conversion to heat, enhancement of fluorescent signals, NIR surface enhanced Raman scattering (SERS) and the ability to conjugate and incorporate therapies or imaging-tracers. This tuning capability combined with the programmable tissue targefing affords the Integrafion of multiple funcfionalities into a single NA and serves as a complementary and non-mutually exclusive tool among different applicafions, including chemotherapy targeting and molecular imaging. Project 4 aims to develop the ligand-directed Si particles- phage- Au particles NAs as novel systems for targeted imaging and therapy in endocrine pancreatic tumors. These efforts will be translated into wide-ranging clinical applications.