Research Support Core: Inhalation Toxicology Core

在工业城市、五大湖附近、正在进行疏浚的地区和修复场地吸入半挥发性多氯联苯，可能会对人类和动物造成神经、生殖和免疫毒性。建立吸入毒理学中心的主要目标是为项目和其他研究支持中心提供最先进的动物设施，并严格控制吸入接触空气中PCB同系物的实际混合物。该核心的具体目标是：目标1)在暴露室中产生受控的PCB气氛，并收集由常见工业PCB混合物汽化产生的空气传播的PCB（支持项目1,2,3,5以及分析和合成核心）；目标2)对实验室动物进行急性、亚急性和亚慢性吸入暴露于大气中规定的多氯联苯混合物和个别同系物（支持项目1和2）；目标3)为用于isbrp的实验动物提供啮齿动物的栖息地，并执行质量保证计划，以监测它们的健康和福利（支持项目1、2和3）；目标4)对暴露、控制和哨点啮齿动物进行尸检，并将组织分发给isbrp的项目调查员（支持项目1、2和3）。吸入毒理学核心位于环境健康科学研究中心的肺毒理学设施（PTF）内，由6000平方英尺的连续实验室和办公室组成。PTF是全国公认的创新动物模型和暴露输送系统。一整套吸入毒理学，气溶胶

Inhalation exposure to semi-volatile PCBs in industrial cities, near the Great Lakes, in areas where dredging is occurring and at remediation sites has the potential to cause neurological, reproductive, and immunological toxicity in humans and animals. The Inhalation Toxicology Core was established with the primary goal to serve the projects and other research support cores with state-of-the-art animal facilities and rigorously controlled inhalation exposures to realistic mixtures of airborne PCB congeners. The specific aims of this Core are: Aim 1) Generate controlled PCB atmospheres in exposure chambers and collect airborne PCBs resulting from vaporization of common industrial PCB mixtures (supports Projects 1, 2, 3, 5 and the Analytical and Synthesis Cores); Aim 2) Perform acute, subacute and subchronic inhalation exposures of laboratory animals to defined mixtures of atmospheric PCBs and to individual congeners, (supports Projects 1 and 2); Aim 3) Provide a rodent vivarium for experimental animals used for the isbrp and execute a quality assurance program to monitor their health and welfare (supports Projects 1, 2, and 3); Aim 4) Perform necropies on exposed, control and sentinel rodents and distribute tissues to project investigators in the isbrp (supports Projects 1,2, and 3). The Inhalation Toxicology Core exists within the Pulmonary Toxicology Facility (PTF) of the Environmental Health Sciences Research Center and consists of 6,000 ft2 of contiguous laboratories and offices. The PTF is nationally recognized for innovative animal models and exposure delivery systems. A full array of inhalation toxicology, aerosol science, and bioassay services are rendered. The Inhalation Toxicology Core is well equipped for generating and quantifying PCB vapors or aerosols; for performing nose-only inhalation exposures of rats and mice in acute, subacute or subchronic protocols; and for determining the degree of exposure and response. A new aim in this renewal is: Aim 5) Perform airborne exposures to primary alveolar and airway epithelia and epithelial cell lines in Transwells with an apical air interface using vapor phase and particle bound PCB congeners. This will bring to the program a new approach for studying toxic effects of airborne PCBs on epithelial cells.