Research Support Core: Inhalation Toxicology Core

在工业城市、五大湖附近、正在进行疏浚的地区和修复地点吸入半挥发性多氯联苯，可能会对人和动物造成神经、生殖和免疫毒性。建立吸入毒理学核心的主要目标是服务于项目和其他研究支持核心，拥有最先进的动物设施，并严格控制吸入暴露于空气中多氯联苯同系物的真实混合物。该核心的具体目标是：目标1)在曝光室产生受控的多氯联苯大气，收集常见工业多氯联苯混合物蒸发产生的气载多氯联苯(支持项目1、2、3、5以及分析和合成核心)；目标2)对实验室动物进行急性、亚急性和亚慢性吸入暴露于确定的大气多氯联苯混合物和个别同系物(支持项目1和2)；目标3)为用于isbrp的实验动物提供啮齿动物空间，并执行质量保证计划以监测它们的健康和福利(支持项目1、2和3)；目的4)对暴露的、对照的和哨兵啮齿动物进行尸检，并将组织分发给ISBRP的项目调查人员(支持项目1、2和3)。吸入毒理学核心存在于环境健康科学研究中心的肺毒理学设施(PTF)内，由6,000平方英尺的毗连实验室和办公室组成。PTF因创新的动物模型和暴露传递系统而在全国范围内获得认可。全套吸入毒理学、气雾剂 科学，并提供生物检测服务。吸入毒理学核心设备齐全，可用于产生和量化多氯联苯蒸气或气雾剂；可用于以急性、亚急性或亚慢性方案对大鼠和小鼠进行鼻部吸入暴露；以及用于确定暴露和反应的程度。此次更新的一个新目标是：目标5)利用气相和颗粒结合的多氯联苯同系物，对Trswells的原始肺泡和呼吸道上皮细胞以及具有顶端空气界面的上皮细胞系进行空气暴露。这将为该计划带来一种研究空气中多氯联苯对上皮细胞毒性影响的新方法。

Inhalation exposure to semi-volatile PCBs in industrial cities, near the Great Lakes, in areas where dredging is occurring and at remediation sites has the potential to cause neurological, reproductive, and immunological toxicity in humans and animals. The Inhalation Toxicology Core was established with the primary goal to serve the projects and other research support cores with state-of-the-art animal facilities and rigorously controlled inhalation exposures to realistic mixtures of airborne PCB congeners. The specific aims of this Core are: Aim 1) Generate controlled PCB atmospheres in exposure chambers and collect airborne PCBs resulting from vaporization of common industrial PCB mixtures (supports Projects 1, 2, 3, 5 and the Analytical and Synthesis Cores); Aim 2) Perform acute, subacute and subchronic inhalation exposures of laboratory animals to defined mixtures of atmospheric PCBs and to individual congeners, (supports Projects 1 and 2); Aim 3) Provide a rodent vivarium for experimental animals used for the isbrp and execute a quality assurance program to monitor their health and welfare (supports Projects 1, 2, and 3); Aim 4) Perform necropies on exposed, control and sentinel rodents and distribute tissues to project investigators in the isbrp (supports Projects 1,2, and 3). The Inhalation Toxicology Core exists within the Pulmonary Toxicology Facility (PTF) of the Environmental Health Sciences Research Center and consists of 6,000 ft2 of contiguous laboratories and offices. The PTF is nationally recognized for innovative animal models and exposure delivery systems. A full array of inhalation toxicology, aerosol science, and bioassay services are rendered. The Inhalation Toxicology Core is well equipped for generating and quantifying PCB vapors or aerosols; for performing nose-only inhalation exposures of rats and mice in acute, subacute or subchronic protocols; and for determining the degree of exposure and response. A new aim in this renewal is: Aim 5) Perform airborne exposures to primary alveolar and airway epithelia and epithelial cell lines in Transwells with an apical air interface using vapor phase and particle bound PCB congeners. This will bring to the program a new approach for studying toxic effects of airborne PCBs on epithelial cells.