Bioanalytical CoreBioanalytical Core

项目概述（见说明）：生物分析核心（Core C）服务于UAB和UCSD的O'Brien中心社区以及扩展的研究基地，为急性肾损伤（AKI）研究提供最先进的生物能量学，氧化应激分析和代谢物分析支持。Core C提供了一个全面的资源，包括氧化应激和细胞生物能量学、AKI生物标志物、翻译后修饰和小分子生物化学变化的生物分析分析的优化方案和技术。服务包括咨询、实验设计培训、回收适合分析的样品和测定性能。提供的新服务包括独特的线粒体生物能量测定技术，基于lc -质谱法的肌酐、f2 -异前列腺素和柠檬酸循环中间体测定。目前正在开发最先进的纳米olc - ms方法，用于在临床样本和AKI动物模型中进行靶向和非靶向代谢组学和外泌体分析。Core C在支持肾脏研究社区方面取得了成功。自Core C成立以来，已为48名主要研究人员进行了8,500多次分析，涉及56个项目。在48名调查人员中，44名是非核心人员。使用Core C的研究人员数量每年都在增加，年度发表率也在增加。Core C还支持了7名试点和可行性补助金获得者的研究工作。这些共同努力目前已在23份同行评议的出版物中得到认可。核心将参与调查人员的教育和培训，提供实践经验和科学交流。2013-2017年的一个反复出现的特征将是一个为期4天的代谢组学培训研讨会（由NIGMS资助，作为NIH代谢组学共同基金计划的一部分）。最后，生物分析核心将与O'Brien中心领导层一起确定研究人员的新生物分析需求并为中心实施。

PROJECT SUMMARY (See instructions): The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the extended research base by providing state-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. New services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. State-of-the-art nanoLC-MS methods are being developed to perform both targeted and untargeted metabolomics and exosome analysis in clinical samples and from animal models of AKI. Core C has been successful in supporting the kidney research community. Since the inception of Core C, more than 8,500 assays have been performed for 48 principal investigators involving 56 projects. Of the 48 investigators, 44 were non-core personnel. The number of investigators using Core C each year is increasing, as is the annual publication rate. Core C has also supported the research efforts of 7 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 23 peer-reviewed publications. The Core will participate in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature from 2013-2017 will be an annual 4-day workshop for training in metabolomics (funded by NIGMS as part of the NIH Common Fund program in metabolomics). Finally, the Bioanalytical Core, in concert with the O'Brien Center leadership, will identify new bioanalytical needs of investigators and implement them for the Center.