Core C - Bioanalytical Resource Core

项目摘要 生物分析核心(核心C)为奥布莱恩中心社区提供服务，包括UAB和UCSD以及 通过提供最先进的生物能量学、氧化应激分析和代谢物来扩展研究基础 以及支持急性肾损伤(AKI)研究的生物标志物分析。Core C提供了全面的 资源，包括氧化应激生物分析的优化方案和技术，以及 细胞生物能量学、AKI的生物标志物、翻译后修饰和小分子的变化 生物化学。这些服务包括咨询、实验设计培训、回收合适的样品 用于分析和化验性能。提供的服务包括确定线粒体的独特技术 生物能量学，基于LC-MS的肌酐、F2-异前列腺素和柠檬酸环的分析 中间体。通过Core C提供的新服务包括肾脏成像质谱仪 组织，用于临床靶向和非靶向代谢组学的最新纳米LC-MS方法 样本和AKI动物模型。 生物分析核心继续为研究人员基础提供独特的服务组合 奥布莱恩中心的。自2013年以来，Core C已经执行了超过53,000次服务/分析，共187次 调查人员涉及226个项目。在187个核心用户中，155个是非核心调查人员。核心C也 为17名试点、可行性和催化剂资助奖获得者提供支持。这些综合努力一直是 在86份同行评议的出版物中得到认可。Core C的工作效率基准显示 &gt；300%用于调查人员(特别是非核心调查人员)、支持的项目以及出版物 与前一个供资周期相比。中心参与对调查人员的教育和培训，提供 实践经验和科学交流。一个反复出现的特点是每年为期4.5天的培训研讨会 在代谢组学方面。生物分析核心与奥布莱恩中心的领导层合作，提供了强大的 支持研究人员推进肾脏相关研究的催化剂。

Project Summary The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the Extended Research bBase by providing state-of-the-art bioenergetics, oxidative stress analysis, and metabolite and biomarker analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. Services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. New services being offered through Core C include imaging mass spectrometry for kidney tissues, state-of-the-art nanoLC-MS methods for both targeted and untargeted metabolomics in clinical samples and from animal models of AKI. The Bioanalytical Core has continued to provide a unique portfolio of services for the investigator base of the O'Brien Center. Since 2013, Core C has performed more than 53,000 services/assays for 187 investigators involving 226 projects. Of the 187 core users, 155 were non-core investigators. Core C also provided support for 17 pilot and feasibility and catalyst grant awardees. These combined efforts have been recognized in 86 peer-reviewed publications. Productivity benchmarks for Core C demonstrate an increase of >300% for investigators (especially non-core investigators), supported projects as well as publications compared to the prior funding cycle. The Core participates in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature is an annual 4.5-day workshop for training in metabolomics. The Bioanalytical Core, in concert with the O'Brien Center leadership, provides a strong catalyst to support investigators to advance kidney-related research.