Elucidating Essential Components of Bone Marrow Metastasis

项目摘要/摘要 在一些致命的癌症中可以观察到骨髓转移。肿瘤细胞优先迁移到 骨腔与骨髓的组成有关。我们已经开发出一种组织工程化的 骨髓的类似物，并计划使用这种结构来识别骨髓细胞的关键基质成分 骨髓转移灶。我们建议：1)开发一个捕获移行肿瘤的微流控平台 体外细胞，2)在芯片上创造肿瘤间质微环境，3)体内比较和治疗 骨髓间充质干细胞支架对肿瘤生长的影响该项目将为候选人提供必要的培训 在K99训练期内的微流体，成像和癌症生物学，并推动他成为一个成功的 生物材料和肿瘤学领域的独立研究员，作为一项研究的R00研究员 上大学。

Project Summary/Abstract Metastasis to the bone marrow is observed in a number of fatal cancers. Tumor cells preferentially migrate to the bone cavity as a function of composition of the bone marrow. We have developed a tissue engineered analogue of the bone marrow and plan to use this construct to identify the key stromal cellular components of a bone marrow metastatic niche. We propose to: 1) Develop a microfluidic platform to capture migratory tumor cells in vitro, 2) Create tumor-stroma microenvironments on-chip, and 3) In vivo comparison and treatment study of BMSC scaffolds on tumor growth. This project will provide essential training to candidate in microfluidics, imaging, and cancer biology during the K99 training period and propel him to be a successful independent investigator in the field of biomaterials and oncology as an R00 investigator at a research university.