Molecular Biology and Cell Core

目的：细胞和分子生物学资源核心的目的是为莫尔豪斯医学院神经科学研究所的研究人员提供最先进的科学资源，这些资源对单个实验室来说成本过高。通过向研究人员提供集中的资源，设备的使用得到了最大化，并以具有成本效益的方式提供给研究人员。关键功能：分子生物学组件为多细胞系统中基因的表达和测量提供支持。这包括一个表达套件，多个实时PCR选项，并获得了高通量基因组研究的生物技术基金资助的Affytron微阵列套件和SOLID 5500 XL DNA测序仪设施。细胞组件通过2个共聚焦显微镜设施提供组织学和成像支持，这些设施专为NI用户提供。通用核心为NI成员提供高端设备，这被认为是尖端研究所必需的，例如超离心机。闪烁计数器。蛋白质组学核心采用最新的沃茨质谱系统，用于蛋白质衍生肽的鉴定和测量。目的：这些核心组件将被U54计划中提出的多个项目所利用，并提供给莫尔豪斯医学院的NI社区的其他成员。这一核心将使研究人员能够获得高成本的共享资源，以进行有竞争力的研究。此外，共同主任将协调关于共用设备的培训。行政管理：核心将由两名联合主任，梅勒博士和周。他们将与应用程序中提出的3个探索性项目中的每个项目的Pi组成一个用户组。该核心将接受由两名共同主任（科学主任和行政主任）组成的行政小组的监督。

Purpose: The purpose ofthe cellular and molecular biology resource core is to provide researchers at the Morehouse School of Medicine Neuroscience Institute access to state of the art scientific resources that are cost prohibitive to a single laboratory. By providing a centralized resource to researchers, use of the equipment is maximized and made available to researchers in a cost efficient manner. Key capabilities: The molecular biology component offers support for the expression and measurement of genes in multiple cell systems. This includes an expression suite, multiple realtime PCR options, and access to the Keck-funded Affymetrix microarray suite and a SOLID 5500 XL DNA sequencer facility for high throughput genomic research. The cellular component offers histology and imaging support via the 2 confocal microscope facilities, which are dedicated for NI users. The general core offers members ofthe NI access to high end equipment, which is considered essential for cutting edge research, for example ultra centrifuges. Scintillation counting an spectrophotometers. The proteomics core features the latest Waters mass spectrometric systems for the identification, and measurement of peptides derived from proteins. Objectives: These core components will be utilized by multiple projects proposed in this U54 program, as well as offered to other members ofthe NI community at Morehouse School of medicine. This core will enable researchers access to high cost shared resources to enable competitive research to be performed. In addition, the co-directors will co-ordinate training on the pieces of shared equipment. Administration: The core will be administered by two co-directors, Dr Meller and Zhou. They will form a user group with the Pi's from each of the 3 exploratory projects proposed in the application. The core will be subject to oversight by an administrative group consisting ofthe two co-directors, the scientific and administrative directors.