Molecular Analysis of Disease Pathways

疾病途径的分子分析

• 批准号: 8691913
• 负责人: Susan M Lunte
• 金额: $ 215.95万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-15 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8691913
• 关键词: Advertisements; Animal Model; Area; Biochemical; Biochemical Pathway; Biological; Biological Markers; Biological Process; Biomedical Engineering; Biomedical Research; Caenorhabditis elegans; Cardiovascular Diseases; Centers of Research Excellence; Chemicals; Chemistry; Collaborations; Core Facility; Custom; Development; Devices; Disease; Disease Pathway; Doctor of Philosophy; Drug Delivery Systems; Educational workshop; Elements; Facility Designs; Faculty; Fluorescent Probes; Genes; Genetic; Genomics; Goals; Housing; Image; Immunoassay; Institutes; Institution; Interdisciplinary Study; Investigation; Kansas; Lung diseases; Malignant Neoplasms; Maps; Mass Spectrum Analysis; Mentors; Methodology; Methods; Microfluidics; Molecular; Molecular Analysis; Molecular Probes; Monitor; Neurosciences; Organism; Pathologic Processes; Pathway interactions; Pharmaceutical Chemistry; Pharmacologic Substance; Phenotype; Physiologic Monitoring; Process; Publications; Reagent; Research; Research Infrastructure; Research Personnel; Research Project Grants; Science; Scientist; Structure; System; Technology; Universities; Zebrafish; base; chemical genetics; chemical synthesis; commercialization; design; empowered; genetic manipulation; implementation research; improved; in vivo; innovation; interest; meetings; mutant; nervous system disorder; new technology; next generation; novel; professor; programs; protein function; public health relevance; screening; symposium; therapeutic target; tool

DESCRIPTION (provided by applicant): The development of new enabling technologies is critical to improving our understanding and treatment of disease. Immunoassays, gene sequencing, and mass spectrometry represent examples of technologies that have revolutionized biomedical research by allowing the identification of biomarkers and disease pathways that could not have been discovered otherwise. We propose here a new COBRE center focused on Molecular Analysis of Disease Pathways. This Center will bring together junior and senior faculty from the physical, biological, and pharmaceutical sciences at the University of Kansas and other academic institutions in Kansas to conduct multidisciplinary research to develop and implement cutting-edge technologies for elucidating the genetic, chemical, and physical mechanisms of biological processes involved in disease. The scientific emphasis ofthe Center will be to create and implement new enabling technologies for identification of therapeutic targets. These enabling methodologies include methods for integrating next generation gene sequencing with genetic manipulation of model organisms, custom-synthesized fluorescent molecular probes for monitoring physiological/pathological processes in model organisms in vivo, and microfluidic systems for manipulation of model organisms and monitoring of biochemical pathways in vivo. A key innovative element of this proposal is the creation of three core facilities that are designed to operate synergistically: imaging of model organisms treated with fluorescent probes developed by one core facility will be facilitated by the use of microfabricated devices developed in a second core facility; screening of mutant organisms such as C. elegans and zebrafish against these probes will be used to discover novel disease-related phenotypes that can be precisely mapped to identify specific targets through the next generation genomic sequencing and related genomic technologies offered by a third core facility. The proposed Center will exploit the strengths of KU and associated universities in the areas of genetics, bioanalytical chemistry, bioengineering, and chemical synthesis and will create new methodologies and approaches that can be used to investigate any pathway involved in disease. These new technologies will be disseminated through research collaborations, publications, and potential commercialization.

描述(由申请人提供)：新的使能技术的发展对于提高我们对疾病的理解和治疗至关重要。免疫分析、基因测序和质谱学代表了一些技术的例子，这些技术通过允许识别否则无法发现的生物标记物和疾病途径，使生物医学研究发生了革命性的变化。在这里，我们建议成立一个新的科布雷中心，专注于疾病途径的分子分析。该中心将汇聚堪萨斯大学和堪萨斯州其他学术机构的物理、生物和制药科学的初级和高级教员，开展多学科研究，开发和实施尖端技术，阐明与疾病有关的生物过程的遗传、化学和物理机制。该中心的科学重点将是创造和实施新的使能技术，以确定治疗目标。这些方法包括将下一代基因测序与模型生物的遗传操作相结合的方法，用于监测体内模型生物的生理/病理过程的定制合成的荧光分子探针，以及用于操纵模型生物和监测体内生化途径的微流控系统。这一提议的一个关键创新要素是建立三个旨在协同运作的核心设施：利用在第二个核心设施开发的微型制造设备，促进对用一个核心设施开发的荧光探针处理的模型生物进行成像；将利用第三个核心设施提供的下一代基因组测序和相关基因组技术，针对这些探针筛选线虫和斑马鱼等突变生物，以发现能够精确绘制图谱以确定特定靶点的新的与疾病有关的表型。拟建的中心将发挥昆士兰大学的优势 他们将在遗传学、生物分析化学、生物工程和化学合成等领域与相关大学合作，并将创建可用于研究与疾病有关的任何途径的新方法和途径。这些新技术将通过研究合作、出版物和潜在的商业化来传播。