Molecular Analysis of Disease Pathways

疾病途径的分子分析

• 批准号: 8883600
• 负责人: Susan M Lunte
• 金额: $ 211.8万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8883600
• 关键词: Advertisements; Animal Model; Area; Biochemical; Biochemical Pathway; Biological; Biological Markers; Biological Process; Biomedical Engineering; Biomedical Research; Caenorhabditis elegans; Cardiovascular Diseases; Centers of Research Excellence; Chemicals; Chemistry; Collaborations; Core Facility; Custom; Development; Devices; Disease; Disease Pathway; Doctor of Philosophy; Drug Delivery Systems; Educational workshop; Elements; Facility Designs; Faculty; Fluorescent Probes; Genes; Genetic; Genomics; Goals; Housing; Image; Immunoassay; Institutes; Institution; Interdisciplinary Study; Investigation; Kansas; Lung diseases; Malignant Neoplasms; Maps; Mass Spectrum Analysis; Mentors; Methodology; Methods; Microfluidics; Molecular; Molecular Analysis; Molecular Probes; Monitor; Neurosciences; Organism; Pathologic Processes; Pathway interactions; Pharmaceutical Chemistry; Pharmacologic Substance; Phenotype; Physiologic Monitoring; Process; Publications; Reagent; Research; Research Infrastructure; Research Personnel; Research Project Grants; Science; Scientist; Structure; System; Technology; Universities; Zebrafish; base; chemical genetics; chemical synthesis; commercialization; design; empowered; genetic manipulation; implementation research; improved; in vivo; innovation; interest; meetings; mutant; nervous system disorder; new technology; next generation; novel; professor; programs; protein function; public health relevance; screening; symposium; therapeutic target; tool

DESCRIPTION (provided by applicant): The development of new enabling technologies is critical to improving our understanding and treatment of disease. Immunoassays, gene sequencing, and mass spectrometry represent examples of technologies that have revolutionized biomedical research by allowing the identification of biomarkers and disease pathways that could not have been discovered otherwise. We propose here a new COBRE center focused on Molecular Analysis of Disease Pathways. This Center will bring together junior and senior faculty from the physical, biological, and pharmaceutical sciences at the University of Kansas and other academic institutions in Kansas to conduct multidisciplinary research to develop and implement cutting-edge technologies for elucidating the genetic, chemical, and physical mechanisms of biological processes involved in disease. The scientific emphasis ofthe Center will be to create and implement new enabling technologies for identification of therapeutic targets. These enabling methodologies include methods for integrating next generation gene sequencing with genetic manipulation of model organisms, custom-synthesized fluorescent molecular probes for monitoring physiological/pathological processes in model organisms in vivo, and microfluidic systems for manipulation of model organisms and monitoring of biochemical pathways in vivo. A key innovative element of this proposal is the creation of three core facilities that are designed to operate synergistically: imaging of model organisms treated with fluorescent probes developed by one core facility will be facilitated by the use of microfabricated devices developed in a second core facility; screening of mutant organisms such as C. elegans and zebrafish against these probes will be used to discover novel disease-related phenotypes that can be precisely mapped to identify specific targets through the next generation genomic sequencing and related genomic technologies offered by a third core facility. The proposed Center will exploit the strengths of KU and associated universities in the areas of genetics, bioanalytical chemistry, bioengineering, and chemical synthesis and will create new methodologies and approaches that can be used to investigate any pathway involved in disease. These new technologies will be disseminated through research collaborations, publications, and potential commercialization.

描述（由申请人提供）：新的使能技术的开发对于改善我们对疾病的理解和治疗至关重要。免疫测定、基因测序和质谱法代表了通过识别生物标志物和疾病途径而彻底改变生物医学研究的技术的例子，这些生物标志物和疾病途径不可能被发现。我们建议在这里建立一个新的COBRE中心，专注于疾病途径的分子分析。该中心将汇集来自堪萨斯大学和堪萨斯其他学术机构的物理、生物和药学科学的初级和高级教师，进行多学科研究，开发和实施尖端技术，以阐明疾病所涉及的生物过程的遗传、化学和物理机制。该中心的科学重点将是创造和实施新的使能技术，用于识别治疗靶点。这些使能方法包括用于将下一代基因测序与模型生物体的遗传操作、用于监测体内模型生物体中的生理/病理过程的定制合成的荧光分子探针、以及用于操纵模型生物体和监测体内生物化学途径的微流体系统整合的方法。该提案的一个关键创新要素是建立三个核心设施，旨在协同运作：使用第二个核心设施开发的微加工装置，将有助于对用一个核心设施开发的荧光探针处理的模式生物进行成像;筛选突变生物，如C。将利用线虫和斑马鱼对这些探针的比对发现新的疾病相关表型，这些表型可以通过第三个核心设施提供的下一代基因组测序和相关基因组技术进行精确定位，以确定特定靶点。拟议中的中心将利用KU的优势 该研究所将在遗传学、生物分析化学、生物工程和化学合成领域与相关大学合作，并将创造新的方法和途径，用于研究疾病的任何途径。这些新技术将通过研究合作、出版物和潜在的商业化来传播。