Gene Transfer Core, NIDCR-DIR

在上一财年，GTC产生了3个基因敲除（KO）小鼠模型。自GTC成立以来，共产生了71只KO小鼠模型。2014财年共产生了151个转基因小鼠品系，使该服务启动以来的转基因小鼠品系总数达到2147个。在2013财年至2014财年，GTC引入了新型基因编辑构建物的显微注射，如锌指核酸酶（ZFN）、转录激活子样效应物核酸酶（TALENS）和最近的聚集规则间隔短回文重复序列（CRISPR）。GTC在FY14冷冻保存49只小鼠模型作为精子，其中28只小鼠模型在卫星冷冻室（Porter Building）冷冻保存。17只小鼠模型成功地从冷冻精子和胚胎中获得。GTC在FY14中纯化了12只小鼠模型的基因组DNA，并使用PCR鉴定了正确的基因型。正如ZIA的其他报告所指出的，2014财年有许多基于GTC生成的小鼠模型的期刊文章发表。

In the previous fiscal year, the GTC generated 3 knockout (KO) mouse models. Since the inception of the GTC, a total of 71 KO mouse models have been generated. A total of 151 transgenic mouse lines were generated in FY14, bringing the total since inception of this service to 2147 mouse lines. In FY13 through FY14, the GTC introduced microinjection of novel gene-editing constructs such as Zinc Finger Nucleases (ZFN), Transcription Activator-like Effector Nucleases (TALENS) and most recently, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). The GTC cryopreserved 49 mouse models as sperm in FY14, of which 28 mouse models were cryopreserved in a satellite freezer (Porter Building). 17 mouse models were successfully rederived from frozen sperm and embryos. GTC purified genomic DNA from 12 mouse models in FY14 and used PCR to identify the correct genotype.As indicated in others ZIA reports, numerous journal articles were published in FY14 based on the mouse models generated by the GTC.