Characterization of Molecular Pathways in Chronic Pain Conditions

慢性疼痛的分子途径特征

• 批准号: 10685866
• 负责人: Ashok Kulkarni
• 金额: $ 94.38万
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10685866
• 关键词: Acidity; Action Potentials; Adult; Affect; Afferent Neurons; Amino Acids; Analgesics; Animal Model; Animals; Attenuated; Back; Behavior; Behavioral; Biochemical; Biopsy; CRISPR/Cas technology; Calcium; Capsaicin; Cells; Chemical Injury; Chemicals; Cinnamon - dietary; Clinical; Clustered Regularly Interspaced Short Palindromic Repeats; Coupled; Cyclin-Dependent Kinase 5; Cyclin-Dependent Kinase Inhibitor; Data; Detection; Development; Devices; Diabetes Mellitus; Diabetic Neuralgia; Diabetic Neuropathies; Disease; Engineering; Environment; Enzymes; Evaluation; Exhibits; Face; Fire - disasters; Freund' s Adjuvant; Future; Gene Expression; Gene Expression Profiling; Genes; Genetically Engineered Mouse; Goals; Green Fluorescent Proteins; Heart Diseases; Histologic; Human; Hyperactivity; Hyperalgesia; Hyperglycemia; Hypersensitivity; Image; Immune; Individual; Inflammation; Inflammation Mediators; Inflammatory; Injections; Ion Channel; Kinetics; Lead; Light; Malignant Neoplasms; Measures; Metabolic; Molecular; Mus; Nervous System Physiology; Neurodegenerative Disorders; Neuronal Plasticity; Neurons; Neuropathy; Nociception; Nociceptors; Olfactory Nerve; Opioid; Organ Donor; Orofacial Pain; Pain; Pain Research; Pathologic; Pathology; Pathway interactions; Patients; Peptides; Persistent pain; Persons; Phosphotransferases; Physiological; Plants; Play; Process; Productivity; Property; Proteomics; Publishing; Quality of life; Reporting; Research; Rodent; Rodent Model; Role; Sampling; Signal Pathway; Skin; Societies; Spinal Cord; Spinal Ganglia; Stimulus; Structure; Structure of trigeminal ganglion; Symptoms; TRP channel; TRPV1 gene; Techniques; Testing; Therapeutic Effect; Tissues; Transgenic Mice; Transgenic Organisms; Trigeminal nerve structure; United States; Up-Regulation; Work; addiction; allodynia; base; behavior test; behavioral response; behavioral study; calcium indicator; cell injury; cell type; chronic pain; chronic pain patient; chronic painful condition; cohort; cost; differential expression; disability; experience; experimental study; functional genomics; in vivo; inflammatory marker; inflammatory pain; inhibitor; interest; macrophage; mammalian genome; metabolomics; mouse genome; mouse model; multiple omics; mustard oil; neurodevelopment; neuron loss; neuronal cell body; neurophysiology; neurotransmitter release; non-diabetic; orofacial; pain sensitivity; pain signal; painful neuropathy; patch clamp; receptor; response; success; tissue injury; tool; transcriptomics

Our lab has acquired DRGs from patients that have been impacted with chronic pain, which affects over 100 million people in the United States. Chronic pain can be difficult to treat with most patients finding even opioids to be ineffective. To study the cellular mechanisms that can lead to chronic pain, we acquired DRGs from patients exhibiting painful diabetic neuropathy (DPN). DPN commonly results from systemic metabolic imbalances such as hyperglycemia that can be toxic to neurons. Pain is often considered the most bothersome symptom for patients with diabetic neuropathy, and is commonly described as sharp, burning, and tingling. DRGs from individuals with painful diabetic neuropathy underwent transcriptomic and histological evaluation to identify possible pathological mechanisms that could be producing pain hypersensitivity. The DRG itself is heterogeneous and is comprised of not just nociceptors, but also of accompanying glial, perivascular, and resident immune cells, where our transcriptomic analysis would identify gene expression changes within these cell types that may participate in the progression of painful neuropathy. An upregulation of many inflammatory markers was detected in the individuals with painful diabetic neuropathy. Some of these inflammatory pathways can have excitatory effects on DRG neurons that could possibly contribute to ongoing pain. Skin biopsies from patients with diabetic neuropathy often show that nociceptive neurons start dying-back, even at early stages in the disease, and our transcriptomic analysis likewise shows an associated loss in neuronally related gene expression in the DRGs of individuals experiencing neuropathic pain. Sections of the DRGs were next histologically examined to look for any pathology that would corroborate our transcriptomic findings of increased inflammation combined with the decreased expression of neuronal genes. Indeed, neuronal loss was detected in 4 out of the 5 DPN subjects that ranged from mild to severe. Immunostaining also detected increases in the number of macrophages in the DRG of those with DPN. Macrophages are immune cells that, when activated, produce inflammatory mediators that can contribute to neuropathic pain. Further biochemical analyses will be conducted using these DRGs to better identify cellular changes that lead to pain hypersensitivity in patients with diabetic neuropathy. In addition, other painful disorders will be examined using DRGs from chronic pain patients. Again, mouse models will be used to validate some of the findings discovered in the human studies. Through our past work with genetically engineered mice, our lab has shown that the activity of the key neuronal enzyme cyclin dependent kinase 5 (Cdk5) is important in regulating of pain hypersensitivity. Cdk5 activity has been shown to have important roles in neurodevelopment and neurophysiology, but aberrant kinase activity has been implicated in neurodegenerative diseases and in cancer. Because of Cdk5's important role in neurological functions such as neurotransmitter release, behavior, and addiction, we wanted to see if Cdk5 activity was also involved in pain. We demonstrated in mice that inflammation causes increased Cdk5 enzymatic activity in nociceptors, both within DRG neurons that innervate the periphery as well as within TG neurons that respond to orofacial inflammation. We then tested the pain responses of genetically engineered mice exhibiting either Cdk5 hyperactivity or decreased Cdk5 activity. Our behavior testing showed that Cdk5 activity modulates thermo-, mechano-, and chemo- nociception in mice, where increased Cdk5 activity promotes hyperalgesia to various noxious stimuli while decreased Cdk5 activity conversely results in hypoalgesia. Essentially, inflammation can induce Cdk5 activity that will, in turn, promote increased pain hypersensitivity, while inhibitors of Cdk5 may have analgesic properties, as suggested in our behavioral studies. Our lab has next identified three key pain transducing ion channels that are affected by Cdk5 activity. Cdk5 phosphorylates both TRPV1, a thermosensitive ion channels that is activated by noxious heat and acidity, along with another TRP channel, TRPA1, which functions as a chemosensor to detect the presence of noxious chemicals in the environment such as the pungent plant defensive compounds found in mustard oil and cinnamon. In addition, Cdk5 also phosphorylates P2X2a, an ion channel that detects cell damage. By phosphorylating these pain transducing ion channels, Cdk5 then modulates their activity that, in turn, plays a part in causing nociceptor hypersensitivity to heat, noxious chemicals, and tissue injury. To further our pain studies involving Cdk5, we also wanted to visualize and record the nociceptor firing of TG neurons of mice in response to painful stimuli. As mentioned, we identified the pain transducing receptors TRPV1, TRPA1, and P2X2a as substrates of Cdk5, and, when activated, these ion channels open to allow both Na+ and Ca2+ to enter into a cell. This, in turn, causes the neuron to depolarize and leads to an action potential. The entry of Ca2+ into the cell can be fluorescently detected using calcium indicators. Currently, we are using a genetically encoded calcium indicator that is transgenically expressed only in pain sensing neurons. Then, we image neuronal responses to both noxious (i.e., heat) and non-noxious (i.e., light brush) stimuli in our genetically engineered mice. This technique is based on a modified green fluorescent protein that will only fluoresce in the presence of calcium. Unlike patch clamp recordings of an individual neuron, we were able to see how multiple neurons respond to a noxious stimulus. In this way, we were able to determine that Cdk5 activity can affect the number of neurons that fire upon application of brush, heat, and capsaicin when applied facially onto a mouse. In particular, the Cdk5 substrate TRPV1 is activated by both heat and capsaicin, so this technique allows us to now visualize how modifying Cdk5 activity in mice may then affect the activation kinetics of this receptor in vivo. As such, mice with engineered Cdk5 hyperactivity showed both stronger neuronal responses and higher numbers of activated neurons to orofacial application of heat and capsaicin, while mice with a 80-90% decrease in Cdk5 activity showed the opposite effects. We then used the inflammatory agent complete Freunds adjuvant (CFA) to induce Cdk5 activity and determine if a peptide inhibitor can reduce nociceptor activity. TFP5 is a 24 amino acid peptide that has been shown to reduce Cdk5 hyperactivity back to normal physiological levels. Either applied directly onto the TG or injected in mice, TFP5 was able to reduce neuronal hyperexcitability caused by injection of the inflammatory agent CFA. Additional mouse models of orofacial pain are being planned for future imaging experiments. In summary, our lab has been interested in understanding various aspects of chronic pain, from developing new mouse models mimicking these painful conditions, to identifying a key neuronal enzyme that can modulate pain sensitivity, and with an analysis of gene expression changes from the DRGs of patients with chronic pain conditions. Our lab will continue to develop and analyze new mouse models to examine pain. We also plan to use data acquired from human patients with chronic pain to identify new avenues of pain signaling research. Lastly, we intend to examine if inhibiting Cdk5 activity may have a therapeutic effect that will attenuate chronic pain.

我们的实验室从患有慢性疼痛的患者那里获得了DRGs，在美国有超过1亿人受到慢性疼痛的影响。慢性疼痛很难治疗，大多数患者发现即使是阿片类药物也无效。为了研究导致慢性疼痛的细胞机制，我们从患有疼痛性糖尿病神经病变（DPN）的患者中获得了DRGs。DPN通常由全身性代谢失衡引起，如高血糖，可对神经元产生毒性。疼痛通常被认为是糖尿病神经病变患者最令人烦恼的症状，通常被描述为尖锐、灼烧和刺痛。对疼痛性糖尿病神经病变患者的DRGs进行转录组学和组织学评估，以确定可能产生疼痛超敏反应的病理机制。DRG本身是异质性的，不仅由伤害感受器组成，还包括伴随的神经胶质细胞、血管周围细胞和常驻免疫细胞，我们的转录组学分析将确定这些细胞类型中的基因表达变化，这些细胞类型可能参与疼痛性神经病变的进展。在疼痛性糖尿病神经病变的个体中检测到许多炎症标志物的上调。其中一些炎症通路可能对DRG神经元有兴奋作用，这可能导致持续的疼痛。糖尿病神经病变患者的皮肤活检经常显示，即使在疾病的早期阶段，伤害性神经元也开始死亡，我们的转录组学分析同样显示，在经历神经性疼痛的个体的drg中，神经相关基因表达的相关损失。接下来对DRGs切片进行组织学检查，以寻找任何病理，以证实我们的转录组学发现，即炎症增加与神经元基因表达减少相结合。事实上，5名DPN受试者中有4名检测到神经元丢失，从轻度到重度不等。免疫染色还检测到DPN患者DRG中巨噬细胞数量增加。巨噬细胞是一种免疫细胞，当被激活时，会产生炎症介质，从而导致神经性疼痛。进一步的生化分析将使用这些DRGs来更好地识别导致糖尿病神经病变患者疼痛超敏反应的细胞变化。此外，还将利用慢性疼痛患者的drg检查其他疼痛障碍。同样，小鼠模型将用于验证在人类研究中发现的一些发现。