Gene Transfer Core, NIDCR-DIR

基因转移核心，NIDCR-DIR

• 批准号: 9155635
• 负责人: Ashok Kulkarni
• 金额: $ 127.13万
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9155635
• 关键词: Biological Assay; Biological Preservation; Biopsy; Chimera organism; Clustered Regularly Interspaced Short Palindromic Repeats; Consultations; Cryopreservation; Embryo; Embryo Transfer; Gene Targeting; Gene Transfer; Generations; Genes; Genomic DNA; Genotype; Injection of therapeutic agent; Knock-out; Mus; National Institute of Dental and Craniofacial Research; Publishing; Reporting; Research; Research Personnel; Services; Tail; Time; Transgenic Mice; Transgenic Organisms; base; embryonic stem cell; gain of function; journal article; loss of function; mouse model; research study; sperm cell

Pronuclear injection service: 92 transgenic constructs were injected to generate 221 transgenic lines with an average turnaround time of 7 weeks. CRISPR-based gene editing knockout service: GTC initiated injections for CRISPR-based gene editing constructs. The average efficiency for generating KO and KI mice for CRISPR constructs was 43% and 3%, respectively. A total of 27 lines were generated from this service. Cryopreservation and IVF services: Sperms from 67 mouse models were cryopreserved for 17 investigators. 23 sperm straws were thawed to use for 63 IVF sessions. 23 mouse models were successfully re-established per investigators request from the sperm cryopreservation service. ES cell injection service: 6 clones from 5 projects were injected to generate 27 chimeric mice with an average turnaround time of 4 weeks. The endpoint of this service was to provide these chimeras to PI. Using these chimeras, 2 heterozygous mouse models were generated by PIs. Genotyping service: 1215 tail biopsies were purified and used for genotyping by PCR analysis for 3 investigators. As indicated in others ZIA reports, numerous journal articles were published in FY15 based on the mouse models generated by the GTC.

原核注射服务：注射92个转基因构建物，产生221个转基因系，平均周转时间为7周。