Gene Transfer Core, NIDCR-DIR

点击翻译按钮获取中文摘要

In FY20, Gene Transfer Core successfully provided following services: Pronuclear injection service: 18 transgenic constructs were injected to generate 35 transgenic lines. CRISPR-based gene editing knockout service: The average efficiency for generating KO and KI mice for CRISPR-based constructs was 67% and 45%, respectively. A total of 31 transgenic mouse lines were generated by this service. Cryopreservation and IVF services: Sperm from 19 mouse models were cryopreserved for 10 investigators. Sperm from all mouse models were stored in two separate buildings. 26 sperm straws were thawed for 96 IVF sessions. 7 mouse models were successfully re-established per investigators request by the sperm cryopreservation service. Genotyping service: 510 tail biopsies were purified and used for genotyping by PCR analysis for 3 investigators. Gene Transfer Cores (GTC) services were shut down during the last 3 months of the fiscal year due to COVID-19 concerns.