Gene Transfer Core, NIDCR-DIR

在2020财年，基因转移核心公司成功地提供了以下服务： 原核注射服务：注射18个转基因构建体，产生35个转基因株系。 基于CRISPR的基因编辑敲除服务：基于CRISPR的构建物生成KO和KI小鼠的平均效率分别为67%和45%。这项服务共产生了31个转基因小鼠品系。 冷冻保存和体外受精服务：19只小鼠模型的精子被冷冻保存，供10名研究人员使用。所有小鼠模型的精子都被储存在两个独立的建筑中。对26根吸管进行了96次体外受精。根据研究人员的要求，精子冷冻保存服务成功重建了7只小鼠模型。 基因分型服务：提取尾部活检组织510份，用于3名调查者的基因分型。 基因转移核心(GTC)服务在本财年的最后3个月因以下原因而关闭 新冠肺炎令人担忧。

In FY20, Gene Transfer Core successfully provided following services: Pronuclear injection service: 18 transgenic constructs were injected to generate 35 transgenic lines. CRISPR-based gene editing knockout service: The average efficiency for generating KO and KI mice for CRISPR-based constructs was 67% and 45%, respectively. A total of 31 transgenic mouse lines were generated by this service. Cryopreservation and IVF services: Sperm from 19 mouse models were cryopreserved for 10 investigators. Sperm from all mouse models were stored in two separate buildings. 26 sperm straws were thawed for 96 IVF sessions. 7 mouse models were successfully re-established per investigators request by the sperm cryopreservation service. Genotyping service: 510 tail biopsies were purified and used for genotyping by PCR analysis for 3 investigators. Gene Transfer Cores (GTC) services were shut down during the last 3 months of the fiscal year due to COVID-19 concerns.