Gene Transfer Core, NIDCR-DIR

基因转移核心，NIDCR-DIR

• 批准号: 10920222
• 负责人: Ashok Kulkarni
• 金额: $ 80.26万
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10920222
• 关键词: Biological Assay; Biopsy; Clone Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Consultations; Cryopreservation; Embryo; Embryo Transfer; Gene Targeting; Gene Transfer; Generations; Genes; Genomic DNA; Genotype; Injections; Knock-in Mouse; Knock-out; Knockout Mice; Mus; National Institute of Dental and Craniofacial Research; Principal Investigator; Research; Research Personnel; Services; Tail; Transgenic Mice; Transgenic Organisms; embryonic stem cell; experimental study; gain of function; loss of function; mouse model; preservation; sperm cell; sperm cryopreservation; tool

In FY23, the Gene Transfer Core successfully provided following services: Pronuclear injection service: 7 CRISPR-based gene editing constructs were injected to generate 34 transgenic lines. CRISPR-based gene editing service: The average efficiency for generating KO and KI mice for CRISPR-based constructs was 25% and 19%, respectively. Cryopreservation and IVF services: Sperm from 36 mouse models were cryopreserved for 9 Principal investigators (21 investigators). Sperm from all mouse models were stored in two separate buildings. 16 sperm straws were thawed for 52 IVF sessions. Genotyping service: 587 tail biopsies were purified and used for genotyping by PCR analysis.

在23财年，基因转移中心成功地提供了以下服务：