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Gene Transfer Core, NIDCR-DIR

Gene Transfer Core, NIDCR-DIR
基因转移核心，NIDCR-DIR
批准号：
10685891
负责人：
Ashok Kulkarni
金额：
$77.91万
依托单位：
NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10685891
关键词：
Biological AssayBiopsyClone CellsClustered Regularly Interspaced Short Palindromic RepeatsConsultationsCryopreservationEmbryoEmbryo TransferGene TargetingGene TransferGenerationsGenesGenomic DNAGenotypeInjectionsKnock-in MouseKnock-outKnockout MiceMusNational Institute of Dental and Craniofacial ResearchPrincipal InvestigatorResearchResearch PersonnelServicesTailTransgenic MiceTransgenic Organismsbaseembryonic stem cellexperimental studygain of functionloss of functionmouse modelpreservationsperm cellsperm cryopreservationtool

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英文摘要
In FY22, the Gene Transfer Core successfully provided following services: Pronuclear injection service: 13 CRISPR-based gene editing constructs were injected to generate 19 transgenic lines. CRISPR-based gene editing service: The average efficiency for generating KO and KI mice for CRISPR-based constructs was 20% and 23%, respectively. Cryopreservation and IVF services: Sperm from 34 mouse models were cryopreserved for 8 Principal investigators (10 investigators). Sperm from all mouse models were stored in two separate buildings. 16 sperm straws were thawed for 20 IVF sessions. Genotyping service: 480 tail biopsies were purified and used for genotyping by PCR analysis.
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Molecular Roles of TGF-beta Signaling in Salivary Glands and Oral Cancer
Molecular Roles of Cdk5 in Neuronal Functions and Pain Signaling
Molecular Genetics of Craniofacial Development and Disease
Characterization of Molecular Pathways in Chronic Pain Conditions
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