Pannoramic 250 BF Flash Tissue Microarray system

Pannoramic 250 BF 闪存组织微阵列系统

• 批准号: 8640732
• 负责人: Kelli L. Boyd
• 金额: $ 45.33万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640732
• 关键词: Automation; Basic Science; Biological Assay; Biological Markers; Breast; Clinical Research; Collaborations; Collection; Colorectal; Communicable Diseases; Computer software; Consumption; Donor Selection; Funding; Gastroenterology; Genomics; Head and neck structure; Hematoxylin and Eosin Staining Method; Image; Image Analysis; Internet; Kidney Diseases; Malignant neoplasm of lung; Malignant neoplasm of prostate; Manuals; Mass Spectrum Analysis; Metadata; One-Step dentin bonding system; Pancreas; Pathology; Patients; Preparation; Process; Productivity; Prostate; Proteins; Proteomics; Research Personnel; Resource Sharing; Sampling; Savings; Scientist; Slide; Staining method; Stains; Standardization; System; Systems Integration; Testing; Thyroid Gland; Time; Tissue Microarray; Tissue Sample; Tissues; United States National Institutes of Health; Validation; base; cohort; design; digital; digital imaging; experience; improved; interest; melanoma; novel; prevent; screening; success; tool

DESCRIPTION (provided by applicant): The Translational Pathology Shared Resource (TPSR) is requesting funds for a high throughput automated tissue microarray system, the TMA Grandmaster-Panoramic 250 system (TMAG/P250). This system is designed for outstanding productivity partnered with the highest possible precision in array preparation. In addition to automation of labor intensive tissue microarray (TMA) preparation and improved standardization, there are other major benefits to the TMAG/P250 integration. The P250 produces a digital image of an H&E stained section of the donor block that is imported into the TMAG and co-registered with an image of the donor block. This effectively consolidates tissue core selection and donor block annotation into one step which equates to significant time savings and more importantly increased accuracy in core selection. Using Case Center software, the digital slide images can be viewed and annotated for tissue core collection from any internet access point which makes collaboration opportunities virtually infinite. Post-processing, the metadata from the TMAG can be exported for a continuous dataflow, which eliminates date entry errors and facilitates rapid image analysis. There is no other comparable high throughput system available for Vanderbilt investigators. TMAs represent a powerful tool for analyzing large patient cohorts for target proteins while minimizing the consumption of valuable tissue. Hundreds of tissue samples on one slide can be tested under uniform experimental conditions preventing interassay variability. Considerable success in the fields of proteomics, genomics, and mass spectrometry depends on validation in tissue based assays and has resulted in increased demand for TMAs. TMAs for melanoma, colorectal, pancreatic, hepatocellular, thyroid, breast, prostate, head and neck, and lung cancers have been constructed in the TPSR using a manual Beecher arrayer. Manual TMA construction and analysis is tedious and time consuming and has limited their use. The major users in this application are highly successful NIH-funded scientists that have previous experience with TMAs, currently utilize TMAs, or have planned projects that require TMA preparation. These users represent a cross section of Vanderbilt investigators engaged in clinical and basic research with diverse interests including melanoma, lung cancer, renal disease, infectious disease, prostate cancer, and gastroenterology. The TMAG/P250 system will accelerate their rate of discovery by providing a platform for high through-put screening of novel biomarkers on large sample groups. .

描述（由申请人提供）：转化病理学共享资源（TPSR）正在申请高通量自动化组织微阵列系统TMA Grandmaster-Panoramic 250系统（TMAG/P250）的资金。该系统旨在实现出色的生产力，并在阵列制备中实现尽可能高的精度。除了劳动密集型组织微阵列（TMA）制备的自动化和改进的标准化之外，TMAG/P250集成还有其他主要好处。P250生成供体块H&E染色切片的数字图像，该图像被导入TMAG并与供体块的图像共配准。这有效地将组织核心选择和供体块注释合并到一个步骤中，这相当于显著节省时间，更重要的是提高核心选择的准确性。使用Case Center软件，可以从任何互联网接入点查看和注释数字切片图像，以进行组织核心收集，这使得合作机会几乎无限。在后期处理过程中，TMAG的元数据可以导出为连续的数据流，这消除了日期输入错误，并促进了快速图像分析。范德比尔特研究人员没有其他可比较的高通量系统。TMA代表了一种强大的工具，用于分析大型患者队列的靶蛋白，同时最大限度地减少有价值组织的消耗。一个载玻片上的数百个组织样本可以在统一的实验条件下进行测试，防止分析间的差异。在蛋白质组学、基因组学和质谱学领域中的相当大的成功取决于基于组织的测定中的验证，并且已经导致对TMA的需求增加。已在TPSR中使用手动比彻阵列仪构建了黑色素瘤、结肠直肠癌、胰腺癌、肝细胞癌、甲状腺癌、乳腺癌、前列腺癌、头颈癌和肺癌的TMA。手动TMA构造和分析是繁琐和耗时的，并且限制了它们的使用。该应用程序的主要用户是非常成功的NIH资助的科学家，他们以前有过TMA的经验，目前正在使用TMA，或者计划进行需要TMA准备的项目。这些用户代表了从事临床和基础研究的范德比尔特研究人员的横截面，他们的兴趣广泛，包括黑色素瘤、肺癌、肾脏疾病、传染病、前列腺癌和胃肠病学。TMAG/P250系统将通过为大样本组的新型生物标志物的高通量筛选提供平台来加快它们的发现速度。.

项目成果

A DLL3-targeted antibody-drug conjugate eradicates high-grade pulmonary neuroendocrine tumor-initiating cells in vivo.

• DOI: 10.1126/scitranslmed.aac9459
• 发表时间: 2015-08-26
• 期刊: Science translational medicine
• 影响因子: 17.1
• 作者: Saunders LR;Bankovich AJ;Anderson WC;Aujay MA;Bheddah S;Black K;Desai R;Escarpe PA;Hampl J;Laysang A;Liu D;Lopez-Molina J;Milton M;Park A;Pysz MA;Shao H;Slingerland B;Torgov M;Williams SA;Foord O;Howard P;Jassem J;Badzio A;Czapiewski P;Harpole DH;Dowlati A;Massion PP;Travis WD;Pietanza MC;Poirier JT;Rudin CM;Stull RA;Dylla SJ
• 通讯作者: Dylla SJ