NIDCR DIR Scientific Cores (Combined Technical Research Core)

NIDCR的口腔科学核心为从事牙科、口腔和颅面疾病研究的校内研究人员提供了研究支持。 联合技术研究中心（CTRC）已进入运作的第二年。CTRC提供最先进的研究技术，科学专业知识和技术支持，以加强所有NIDCR研究人员的研究工作。它提供的服务包括： 1)DNA测序;为NIDCR测序仪主导的研究提供了超过12，000个反应， 2)流式细胞术; 2014年，对15名以上的研究人员进行了细胞计数分析仪培训，并将其使用量从7名增加到20多名研究人员和科学家。两台分析仪之间使用的总小时数超过1350小时。 3)FACS细胞分选;超过28名研究者进行了超过780小时的FACS分选。在过去的一年里，对细胞计数的需求大幅增加，这一点从我们的数据库的增长中可以看出，从只有大约15名研究人员到40多名定期使用该设施进行细胞计数实验的研究人员。 4)细胞淘洗，共淘洗了133种细胞类型，包括单核细胞、PBMC、血小板和T细胞。 5)激光捕获显微镜; LCM已使用超过75小时，但预计研究者将更多地使用。 6）组织学; 2014年2月加入CTRC，支持组织处理、切片和染色。 CTRC预计下一个财政年度对其服务和资源的需求将增加，并希望通过升级现有仪器和增加另一台流式细胞仪来扩大规模。

The NIDCR DIR Scientific Cores provided research support for intramural investigators performing studies on dental, oral and craniofacial diseases. The Combined Technical Research Core (CTRC)is in it's second year of operation. CTRC provides access to state-of-the-art research technologies, scientific expertise, and technical support to enhance the research efforts of all NIDCR DIR investigators. The services that it offers include the following: 1) DNA Sequencing; Provided over 12,000 reactions for NIDCR DIR investigator-led research, 2) Flow Cytometry; Trained over 15 investigators during 2014 on the Cytometry analyzers and increased its usage from only 7 to over 20 investigators and scientists. The total hours used was over 1350 hours between two analyzers. 3) FACS Cell Sorting; FACS sorted over 780 hours for over 28 investigators. The demand for Cytometry usage has increased tremendously over the last year which is evident by the growth of our database from only about 15 investigators to over 40 who use the facility for their Cytometry experiments regularly. 4)Cell Elutriation, have elutriated total of 133 cell types including Monocytes, PBMC, Platelets, and T-Cells. 5)Laser Capture Microscopy; LCM has been used for over 75 hours but is projected to be utilized more by the investigators. 6)Histology; joined the CTRC in February of 2014 and supports tissue processing, sectioning and staining. CTRC projects increase in demand of its services and resources for the next fiscal year and looks to expand by upgrading its current instrumentation and adding another FACS Sorter.