Automated 3D quantitative analysis of dendritic spines imaged with light microsco

使用光学显微镜成像的树突棘的自动 3D 定量分析

• 批准号: 8643290
• 负责人: Paul Angstman
• 金额: $ 59.27万
• 依托单位: MICROBRIGHTFIELD, LLC
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-12 至 2016-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8643290
• 关键词: Acceleration; Address; Agaricales; Algorithms; Anatomy; Autistic Disorder; Automation; Benchmarking; Biomedical Research; Brain; Central Nervous System Diseases; Classification; Code; Collaborations; Communities; Complex; Computer software; Dendrites; Dendritic Spines; Detection; Drug abuse; Elements; Filopodia; Goals; Head; Image; Image Analysis; Impaired cognition; Information Storage; Knowledge; Label; Laboratories; Learning; Light; Maintenance; Marketing; Memory; Mental disorders; Microscope; Microscopic; Modeling; Morphologic artifacts; Morphology; Motion; National Institute of Mental Health; Neuraxis; Neurobiology; Neurodegenerative Disorders; Neurons; Neurosciences; Neurosciences Research; New York; Performance; Phase; Physiological; Prevention; Procedures; Process; Provider; Research; Research Personnel; Research Support; Schizophrenia; Shapes; Slice; Slide; Societies; Structure; Synapses; TNFRSF5 gene; Techniques; Technology; Testing; Three-Dimensional Image; Tissues; Vertebral column; Writing; base; improved; in vivo imaging; innovation; interest; medical schools; novel; portability; programs; prototype; reconstruction; software development; tissue preparation; tool; treatment strategy; validation studies; virtual

DESCRIPTION (provided by applicant): In this Lab to Marketplace proposal, we aim to develop the MBF SpineStudio software incorporating the innovative, laboratory-based NeuronStudio software created at the Mount Sinai School of Medicine. Our SpineStudio software will enable automated detection, reconstruction and morphological classification of the structural analysis of dendritic spines. By creating this commercial software, we will improve the existing technology by providing validated, supported, and fully-documented software for automated 3D quantitative dendritic spine analysis. This software will integrate the specific advantages of the NeuronStudio software for a combination of neuron labeling, imaging (including in vivo), and image pre- processing techniques applied by the user without requiring expert knowledge in software programming. The software product will offer automated detection, reconstruction, and separate classification of complex spines (cup-shaped, multi-headed or branched, filopodia). Providing this innovative functionality will help research in important fields such as neurodegenerative diseases, learning, and memory.

描述（由申请人提供）：在这个从实验室到市场的提案中，我们的目标是开发MBF SpineStudio软件，结合西奈山医学院创建的创新的、基于实验室的NeuronStudio软件。我们的SpineStudio软件将实现树突棘结构分析的自动检测、重建和形态分类。通过创建这个商业软件，我们将通过提供经过验证、支持和完整文档的软件来改进现有技术，用于自动3D定量树突脊柱分析。该软件将集成NeuronStudio软件的特定优势，用于神经元标记，成像（包括体内）和用户应用的图像预处理技术的组合，而不需要软件编程方面的专业知识。该软件产品将提供自动检测、重建和单独分类复杂的脊柱（杯状、多头或分支、丝状足）。提供这种创新功能将有助于神经退行性疾病、学习和记忆等重要领域的研究。