System for advanced automated 3D microvascular analysis in neuroplasticity

用于神经可塑性的先进自动化 3D 微血管分析系统

• 批准号: 9332468
• 负责人: Paul Angstman
• 金额: $ 49.99万
• 依托单位: MICROBRIGHTFIELD, LLC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-06 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9332468
• 关键词: Address; Aging; Algorithms; Biological; Biomedical Research; Biotechnology; Blood Vessels; Brain; Brain Diseases; Collaborations; Communities; Complex; Computer software; Destinations; Development; Dimensions; Encephalitis; Grant; Graph; Image; Image Analysis; Learning; Light; Lighting; Manuals; Marketing; Mental Health; Mental disorders; Michigan; Microscope; Microscopic; National Institute of Mental Health; Nerve Degeneration; Neuronal Plasticity; Neurons; Neurosciences; Neurosciences Research; New York; Optics; Pathologic; Performance; Periodicity; Pharmacology; Phase; Physiological; Play; Process; Production; Reporting; Reproducibility; Research; Research Personnel; Role; Scientist; Small Business Innovation Research Grant; Societies; Software Tools; Structure; Synaptic plasticity; System; Systems Analysis; Techniques; Technology; Testing; Three-Dimensional Image; Three-dimensional analysis; Time; Tissues; Traumatic Brain Injury recovery; Trees; United States National Institutes of Health; Universities; Validation; advanced system; brain morphology; brain tissue; cerebral microvasculature; commercialization; data modeling; digital; experience; fighting; improved; innovation; insight; medical schools; microscopic imaging; migration; network models; neurogenesis; novel; research and development; technology development; therapeutic development; tool; treatment strategy; two-photon; usability

Abstract It has become evident that the microvasculature (i.e., microscopically small blood vessels) plays a critical role in the plasticity of the brain for fundamental processes: (i) neurogenesis, (ii) migration of neuronal precursors to their final destination in the brain, (iii) axonal, dendritic and synaptic plasticity during brain development, learning, aging and neurodegeneration, and (iv) recovery from traumatic brain injury and brain inflammation. For more than thirty years, investigators have attempted to trace, reconstruct, visualize and quantitatively characterize the three-dimensional (3D) morphology of the brain's microvasculature in normal and pathological tissue (3D microangioarchitectonics). Yet, 3D microangioarchitectonics is rarely used in neuroscience research due to the paucity of adequate tools. The investigators who have started to study 3D microangioarchitectonics use software tools that were developed for other purposes. Because these software tools make use of data models which are inappropriate for 3D microangioarchitectonics and have not been validated against a manually established ground truth, the results must be considered faulty and irreproducible. To remedy this untenable situation we propose to create Vesselucida, an innovative software product to perform advanced, accurate and reproducible automatic 3D microangioarchitectonics in normal and pathological brain tissue. This software will allow significant advancements in (i) neuroscience research that addresses the roles of microvessels on various aspects of neuroplasticity, and (ii) pharmacological and biotechnology research and development. These advancements will be the basis for the development of innovative treatments to fight complex brain diseases. During Phase I we successfully established proof of concept and demonstrated that the development of Vesselucida represents substantial progress beyond the state-of-the-art, with great benefits for the neuroscience research community and society in general.

摘要 已经明显的是微脉管系统（即，显微镜下的小血管）在 大脑的可塑性的基本过程：（一）神经发生，（二）迁移的神经元前体， 它们在大脑中的最终目的地，（iii）大脑发育，学习， 老化和神经变性，和（iv）从创伤性脑损伤和脑炎症中恢复。有关详细 三十多年来，研究人员试图追踪，重建，可视化和定量表征 正常和病理组织（3D）中脑微血管的三维（3D）形态 微血管构造学）。然而，3D微血管构筑学很少用于神经科学研究，因为 缺乏足够的工具。已经开始研究3D微血管结构的研究人员使用软件 为其他目的开发的工具。因为这些软件工具使用的数据模型是 不适合3D微血管架构，并且尚未针对手动建立的模型进行验证 地面真相，结果必须被认为是错误的和不可复制的。为了纠正这种难以维持的局面， 我建议创建Vesselucida，一个创新的软件产品，以执行先进，准确和可复制的 在正常和病理脑组织中的自动3D微血管构筑。该软件将允许大量 （一）神经科学研究的进展，解决了微血管在各个方面的作用， 神经可塑性，以及（ii）药理学和生物技术研究与开发。这些进步 将成为开发创新疗法以对抗复杂脑部疾病的基础。在阶段I期间 我们成功地建立了概念验证，并证明了Vesselucida的发展代表了 超越最先进技术的实质性进展，为神经科学研究界带来巨大利益 和整个社会。