Analytical Imaging Core (Core B)

项目摘要 分析成像核心(核心B)将继续为该计划项目的四个项目提供服务。 这一核心的长期目标是为基于图像的各个项目的需求提供支持 程序、蛋白质分析和化学生物学。 这个核心的具体目标是：1)提供一个全面的成像设施，以帮助 研究人员在形态研究中需要分析年龄相关变化在自噬中的作用 不同的细胞和动物模型；2)与各个项目的成员合作，以实现 在其实验环境中实施蛋白质分析方法；以及3)启动 基于自噬通路调节的靶向治疗在不同疾病中的应用 这些项目使用的细胞和动物模型。 核心的组成部分是：1)成像股：已经并将继续提供关于以下方面的建议 样品制备/处理以及分析成像的技术和程序，执行 专门的成像程序，并协助解释形态图像；2) 创新单位：探索、测试和实施蛋白质修饰分析和 利用结构和化学生物学开发自噬的化学调节剂。 核心提供的服务包括：一系列全面的分析成像程序(直接和 间接免疫荧光、实时荧光显微镜、共聚焦荧光、常规和 冷冻电子显微镜和免疫金)，获得成像仪器，以及常见的 用于成像程序的试剂(抗体、探针和荧光试剂)。可提供的新服务 在此期间，将包括体层摄影、完整细胞的免疫金/银增强标记、活体内 CMA激活剂的成像、蛋白质聚集体的规定和发展、表征和分布。 关键人员是成像核心的董事、专职技术人员和专家技术人员 在爱因斯坦，他是一位化学医学专家和靶向治疗专家。董事们将监督 核心的所有活动，提供咨询并协助解释结果。技术人员将做好准备 并处理用于形态研究的样品，并维护岩芯所需的补给和试剂。 相关性：成像程序和蛋白质分析是本研究中所有四个项目的基本和共同之处 程序，因为它们允许在整个细胞或器官中研究感兴趣的细胞过程。这个 程序的集中化优化了成本效率，确保了程序的一致性，并允许集成 不同小组获得的数据。将化学生物成分结合到核心中 将促进基于自噬调节的抗衰老疗法的发展。

Project Summary The Analytical Imaging Core (Core B) will continue serving the four projects of this Program Project. The long-term goal of this core is to provide support for the needs of the individual projects on image-based procedures, protein analysis and chemical biology. The specific aims of this core are: 1) to provide a comprehensive imaging facility that will assist the investigators in the morphological studies required to analyze the role of age-related changes in autophagy in different cellular and animal models; 2) to work with the members of the individual projects toward the implementations in their experimental settings of methods for protein analysis; and 3) to initiate the development of targeted therapeutics based on modulation of autophagic pathways for testing in the different cellular and animal models used by the projects. The components of the core are: 1) The Imaging unit: That has and will continue providing advice on sample preparation/processing and techniques and procedures for analytical imaging, performing the specialized imaging procedures, and assisting with the interpretation of morphological images; 2) The innovation unit: That explores, tests and implements procedures for protein modification analysis and for the development of chemical modulators of autophagy using structural and chemical biology. The services offered by the core include: a comprehensive array of analytic imaging procedures (direct and indirect immunofluorescence, real time fluorescence microscopy, confocal fluorescence, conventional and cryoelectron microscopy and immunogold), access to the imaging instrumentation, and a stock of common reagents (antibodies, probes and fluorescent dies) for the imaging procedures. The new services available during this period will include tomography, Immunogold/silver enhancement for intact cells labelling, in vivo imaging, protein aggregates islation and development, characterization and distribution of CMA activators. The key personnel are the directors, a dedicated technician, the expert technical staff of the imaging core at Einstein, an expert in chemical medicine and an expert in targeted therapeutics. The directors will supervise all of the activities of the core, offer advice and assist with result interpretation. The technical staff will prepare and process samples for morphological studies and maintain the supplies and reagents required for the core. Relevance: Imaging procedures and protein analysis are essential and common to all four projects in this program as they allow the study of the cellular process of interest in the whole cells or organs. The centralization of the procedures optimizes cost efficiency, guarantees their uniformity and allows for integration of the data obtained by the different groups. The incorporation of a chemical biology component to the core will facilitate development of anti-aging therapeutics based on autophagy modulation.