TISSUE ACQUISITION, MORPHOLOGY, AND CELL CULTURE

组织采集、形态学和细胞培养

• 批准号: 8834994
• 负责人: Darryl D. D'Lima
• 金额: $ 82.4万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8834994
• 关键词: 3-Dimensional; Address; Adult; Age; Age Distribution; Age-Years; Aging; Biomechanics; Body mass index; Cartilage; Cell Count; Cell Culture Techniques; Cell Density; Cell Separation; Cells; Chondrocytes; Communities; DNA; Data; Databases; Degenerative polyarthritis; Diagnostic; Evaluation; Exhibits; Flow Cytometry; Gender; Genomic DNA; Harvest; Height; Histology; Human; Instruction; Joints; Knee; Knee bone; Knee joint; Lead; Ligaments; Maps; Measurement; Meniscus structure of joint; Messenger RNA; Microscopic; Molecular; Morphology; Pattern; Phenotype; Process; Prognostic Marker; Proteins; RNA; Race; Research; Research Personnel; Research Project Grants; Sampling; Services; Specimen; Surface; Synovial Membrane; System; Techniques; Testing; Therapeutic; Thick; Tissue Banking; Tissue Banks; Tissue Extracts; Tissues; age group; age related; articular cartilage; base; bone; cell bank; human tissue; improved; microCT; multidisciplinary; novel; osteochondral tissue; programs; prophylactic; research study; substantia spongiosa; success

Core B serves the overall function of procuring, processing and distributing human knee tissue and tissue extracts to the 3 research projects. The use of human tissues is a central and unifying part of the proposed experimental approach. Access to sufficient numbers of samples has been successfully maintained since the origin of this program. In the past cycle. Core B refined and standardized sample processing and analysis provided all projects with tissues, cells, and molecular extracts as needed. This service is a key feature and basis of this program. Core B also assumed an ancillary function to generate data for descriptive studies that will support hypothesis driven research. Core B will document the relevant parameters in articular cartilage and other joint tissues providing a multidisciplinary analysis of age-related changes in the knee. All projects can now study the same knee samples across the entire adult age spectrum. In addition to specific themes that are addressed by the 3 projects, the multidisciplinary analysis of the same human samples represents one of the central common approaches in the program. The Specific Aims are to procure human knee joints (100 donors, 200 joints per year) from tissue banks; conduct macroscopic assessment; han/est specimens; perform microscopic assessment including semiquantitative and quantitative histology and histomorphometric measurements; isolate cells (chondrocytes, meniscal synovial, ligament cells); extract RNA, DNA, and protein; perform biomechanical testing; identify patterns of degeneration and determine relationships of changes in all joint tissues. In addition. Core B will store and distribute materials (tissue sections, DNA, RNA, proteins) nationally to other investigators. A major strength of this core is the analysis of the same tissue specimens in multiple experiments with different techniques to test different hypotheses This approach supports direct associations among the different observations and enhances the power of the observations. By performing a standardized evaluation of cartilage, and of all other joint tissues, changes that occur with aging can be identified and the relationship of changes in the different tissues can be determined. Comparison with similar specimens exhibiting OA presents a unique opportunity to define similarities and differences between the process of OA and that of aging. The studies proposed in all research components depend on these samples, and this core is thus essential to the success of the program.

核心B服务于获取、处理和分配人体膝关节组织和组织的总体功能 三个研究项目的摘要。人体组织的使用是所提出的方法的中心和统一部分。 实验方法自2001年以来，成功地保持了对足够数量样本的访问。 这个节目的起源。在过去的周期中。核心B精制和标准化样品处理和分析 根据需要为所有项目提供组织、细胞和分子提取物。这项服务是一项关键功能， 这个方案的基础。核心B还假设辅助功能，以生成描述性研究的数据， 将支持假设驱动的研究。核心B将记录关节软骨中的相关参数 和其他关节组织，为膝关节的年龄相关变化提供多学科分析。所有项目 现在可以研究整个成人年龄范围内的相同膝盖样本。除了具体的主题之外， 这三个项目所解决的问题，对相同人体样本的多学科分析代表了 这是该计划中的一个核心共同方法。具体目标是获得人体膝关节 (100供体，每年200个关节）;进行宏观评估; HAN/EST标本; 进行显微镜评估，包括半定量和定量组织学， 组织形态测量;分离细胞（软骨细胞、关节滑膜、韧带细胞）;提取物 RNA、DNA和蛋白质;进行生物力学测试;识别退化模式并确定 所有关节组织的变化关系。另外。核心B将储存和分发材料（组织 切片，DNA，RNA，蛋白质）全国其他研究人员。这个核心的一个主要优势是分析 用不同的技术在多个实验中对相同的组织标本进行不同的假设检验 这种方法支持不同观测之间的直接关联，并增强了 意见。通过对软骨和所有其他关节组织进行标准化评估， 可以识别随着衰老发生的变化，并且可以确定不同组织中变化的关系， 测定与表现出OA的相似标本进行比较，提供了一个独特的机会来定义 OA和衰老过程的异同。所有研究都建议 研究的组成部分取决于这些样本，因此，这一核心是必不可少的成功， 程序.