TISSUE ACQUISITION, MORPHOLOGY, AND CELL CULTURE

核心B提供获取、加工和分发人类膝关节组织和组织的总体功能 3个研究项目的摘录。使用人体组织是拟议中的核心和统一部分 实验方法。成功地保持了对足够数量的样本的访问，因为 本节目的由来。在过去的周期中。核心B精细化和标准化的样品处理和分析 根据需要为所有项目提供组织、细胞和分子提取物。这项服务是一项关键功能， 这是该计划的基础。核心B还承担了辅助功能，以生成描述性研究的数据 将支持假说驱动的研究。核心B将记录关节软骨的相关参数 和其他关节组织提供了对膝关节年龄相关变化的多学科分析。所有项目 现在可以研究整个成年年龄段的相同膝盖样本。除了特定主题之外 3个项目涉及的问题，对相同的人体样本进行多学科分析代表 这是该计划的主要常见方法之一。具体目标是获取人类膝关节。 来自组织库的(100名捐赠者，每年200个关节)；进行宏观评估；HAN/EST标本； 进行包括半定量和定量组织学在内的显微评估 组织形态计量学测量；分离细胞(软骨细胞、半月板滑膜、韧带细胞)；摘录 RNA、DNA和蛋白质；进行生物力学测试；识别退变模式并确定 所有关节组织变化的关系。此外。核心B将存储和分发材料(组织 切片、DNA、RNA、蛋白质)在全国范围内移交给其他调查人员。这个核心的一个主要优势是分析 在多个实验中使用不同的技术来检验不同的假设 这种方法支持不同观察之间的直接关联，并增强了 观察。通过对软骨和所有其他关节组织进行标准化评估，改变 可以识别随着年龄增长而发生的变化，并且可以确定不同组织中的变化的关系 下定决心。与显示骨性关节炎的类似标本进行比较提供了一个独特的机会来确定 骨性关节炎和衰老过程的异同。所有建议的研究 研究组成部分依赖于这些样本，因此，这一核心是成功的关键 程序。 相关性(请参阅说明)： 该计划的目标是确定导致骨性关节炎的早期机制。该模型的多模态分析 整个膝关节是一个独特的机会来了解骨性关节炎的过程以及与自然衰老的区别。 这将导致改善OA的诊断和预后标志以及新的预防和治疗方法 接近了。核心B将处理和存储组织切片和提取物，并在全国范围内分发给 研究人员，当与宏观、微观和生物力学数据库结合时 由核心维护的测量，将是对科学界的宝贵贡献。

Core B serves the overall function of procuring, processing and distributing human knee tissue and tissue extracts to the 3 research projects. The use of human tissues is a central and unifying part of the proposed experimental approach. Access to sufficient numbers of samples has been successfully maintained since the origin of this program. In the past cycle. Core B refined and standardized sample processing and analysis provided all projects with tissues, cells, and molecular extracts as needed. This service is a key feature and basis of this program. Core B also assumed an ancillary function to generate data for descriptive studies that will support hypothesis driven research. Core B will document the relevant parameters in articular cartilage and other joint tissues providing a multidisciplinary analysis of age-related changes in the knee. All projects can now study the same knee samples across the entire adult age spectrum. In addition to specific themes that are addressed by the 3 projects, the multidisciplinary analysis of the same human samples represents one of the central common approaches in the program. The Specific Aims are to procure human knee joints (100 donors, 200 joints per year) from tissue banks; conduct macroscopic assessment; han/est specimens; perform microscopic assessment including semiquantitative and quantitative histology and histomorphometric measurements; isolate cells (chondrocytes, meniscal synovial, ligament cells); extract RNA, DNA, and protein; perform biomechanical testing; identify patterns of degeneration and determine relationships of changes in all joint tissues. In addition. Core B will store and distribute materials (tissue sections, DNA, RNA, proteins) nationally to other investigators. A major strength of this core is the analysis of the same tissue specimens in multiple experiments with different techniques to test different hypotheses This approach supports direct associations among the different observations and enhances the power of the observations. By performing a standardized evaluation of cartilage, and of all other joint tissues, changes that occur with aging can be identified and the relationship of changes in the different tissues can be determined. Comparison with similar specimens exhibiting OA presents a unique opportunity to define similarities and differences between the process of OA and that of aging. The studies proposed in all research components depend on these samples, and this core is thus essential to the success of the program. RELEVANCE (See instructions): The objective of this program is to identify eariy mechanisms that lead to OA. The multimodal analysis of the entire knee is a unique opportunity to understand the process of OA and the difference from natural aging. This will lead to improved diagnostic and prognostic markers of OA and novel prophylactic and therapeutic approaches. Core B will process and store tissue sections and extracts to be distributed nationally to investigators which, when combined with the database of macroscopic, microscopic, and biomechanical measurements maintained by the core, will be a valuable contribution to the scientific community.