LAB CORE

实验室核心

• 批准号: 8933319
• 负责人: Amr Soliman
• 金额: $ 9.93万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-17 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8933319
• 关键词: Africa; Africa South of the Sahara; Anti-Retroviral Agents; Cervix carcinoma; Collaborations; Core Facility; Country; Coupled; Data; Data Collection; Data Storage and Retrieval; Development; Diagnosis; Diagnostic; Disease; Education; Educational Intervention; Educational workshop; Environment; Exercise; Female; Fostering; Funding; Future; Genotype; Goals; HIV-1; Health Professional; Herpesviridae; Human; Human Herpesvirus 8; Human Papillomavirus; Human papilloma virus infection; Immune System and Related Disorders; Immune response; Incidence; Individual; Infection; Information Technology; Institutes; International; Kaposi Sarcoma; Laboratories; Laboratory Research; Lead; Location; Malignant Neoplasms; Malignant neoplasm of cervix uteri; Medical; Medical center; Methods; Molecular Epidemiology; Molecular Virology; Nebraska; Oceans; Patients; Prevention; Prevention strategy; Process; Quality Control; Research; Research Project Grants; Research Training; Resource Sharing; Resources; Role; Sampling; Serological; Severities; Site; Tanzania; Technology; Training; Universities; Vaccine Design; Vaccines; Viral; Viral Load result; Virus; anticancer research; arm; base; cancer risk; cancer therapy; cost effective; improved; instrumentation; male; quality assurance; virology

The etiologic role of human papilloma virus (HPV) infection in human cervical carcinoma, and of human herpesvirus-8 (HHV-8/KSHV) in Kaposi's sarcoma, is clear. Both malignancies are exacerbated in incidence and severity under the immunological dysfunction induced by HIV-1 co-infection. In regions of sub-Saharan Africa where HIV-1 incidence remains high, infection with either HPV or KSHV/HHV-8 can lead to increased likelihood of malignancy even if HIV-1 is not a contributor and even if retroviral disease is controlled through antiretroviral treatment. At the Ocean Road Cancer Institute (ORCI), Kaposi's sarcoma and cervical cancer are the leading cancers among males and females, respectively, so it is critical to understand the molecular epidemiology and viral genotypes involved in cancer development in the region and to define any potentially effective immune responses against these viruses for potential vaccines or preventive strategies. A partnership between ORCI and two branches of the University of Nebraska has been established to investigate these issues and to increase ORCl's research capacity. The partnership will provide an environment for training and information/technology exchange that will increase the intellectual and research capacity among Tanzanian medical practitioners. This Core will be divided between the Nebraska Center for Virology (NCV) and the ORCI Laboratory Section to provide technical support for the two research arms to be conducted jointly by trainees in Nebraska and Tanzania. This Core will also provide capacity building and foster training and educational opportunities for technology and expertise transfer to Tanzanians in both locations through workshops and hands-on training exercises. Specific aims are to: 1) provide carefully curated patient data collection and storage, coupled with patient sample procurement, on-site processing, and long-term archival storage for all aspects of the Consortium; 2) develop and implement in-country diagnostics for HPV and HHV-8 focused on PCR-based strain differentiation and serological methods, respectively; and 3) serve as an education/research training center for Tanzanian medical fellows and others to support cancer research collaborations and enhance local and regional expertise.

人类乳头瘤病毒（HPV）感染在人宫颈癌中的病因作用，以及人类疱疹病毒8（HHV-8/kSHV）在Kaposi的肉瘤中的病因。在HIV-1共感染引起的免疫功能障碍下，两种恶性肿瘤都在发病率和严重程度加剧。在HIV-1发病率保持较高的撒哈拉以南非洲地区，即使HPV或KSHV/HHV-8感染也可能导致恶性肿瘤的可能性增加，即使HIV-1不是促进者，即使通过抗逆转录病毒治疗控制了逆转录病毒疾病。在海洋公路癌症研究所（ORCI），卡波西的肉瘤和宫颈癌分别是男性和女性中的主要癌症，因此了解参与该地区癌症发展的分子流行病学和病毒基因型至关重要，并且要对这些潜在的疫苗或预防性策略定义任何潜在的有效免疫反应。 ORCI与内布拉斯加州大学的两个分支机构之间建立了伙伴关系，以调查这些问题并提高ORCL的研究能力。该合作伙伴关系将为培训和信息/技术交流提供一个环境，该环境将提高坦桑尼亚医生之间的智力和研究能力。该核心将在内布拉斯加州病毒学中心（NCV）和ORCI实验室区域之间进行分配，以提供技术支持，为两名研究机构由内布拉斯加州和坦桑尼亚的学员共同进行。该核心还将通过研讨会和动手培训练习提供能力建设和培养培训和教育机会，以将技术和专业知识转移给坦桑尼亚人。具体目的是：1）为财团的各个方面提供精心策划的患者数据收集和存储，再加上患者样品采购，现场处理和长期档案存储； 2）分别针对基于PCR的菌株分化和血清学方法开发和实施HPV和HHV-8的国内诊断； 3）作为坦桑尼亚医学研究员和其他人的教育/研究培训中心，以支持癌症研究合作并增强本地和地区专业知识。