Establishing a Bioexclusion Barrier in the Combs Cancer Research Building

在库姆斯癌症研究大楼建立生物排斥屏障

• 批准号: 8902341
• 负责人: HAROLD F STILLS
• 金额: $ 48.4万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8902341
• 关键词: Animal Diseases; Animal Housing; Area; Beds; Burn injury; Comb animal structure; Complex; Cryopreservation; Embryo; Environmental air flow; Facility Designs; Funding; Future; Heating; Helicobacter; Housing; Human Resources; Immunocompromised Host; Infection; Kentucky; Maintenance; Morbidity - disease rate; Mouse Strains; Noise; Norovirus; Odors; Pasteurella pneumotropica; Procedures; Research; Research Infrastructure; Research Personnel; Rodent; Sanitation; Sterilization; System; Time; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Universities; animal facility; anticancer research; design; hazard; improved; mortality; pathogen; prevent; supported housing; vibration

 DESCRIPTION (provided by applicant): The University of Kentucky requests $500,000 to be combined with a $441,449 university contribution to establish a bioexclusion barrier capability on the east campus biomedical complex by renovating a portion of the Combs Cancer Research Building (CCRB) animal facility. The CCRB animal facility is a 5,570 ft2 facility that is an integrl portion of the ~34,000 ft2 contiguous east campus biomedical complex animal facility and contains the cage sanitation and sterilization support facilities for all rodents housed in the entre facility. The east campus biomedical complex animal facility is an older facility designed for conventional animal housing. The minimal sterilization capacity and lack of restricted entry barrier housing rooms with integral procedural rooms has severely limited our ability to prevent the exposure of rodents to endemic opportunistic pathogens including Pasteurella pneumotropica, Helicobacter spp. and Norovirus. The result has been morbidity and mortality in unique immunocompromised transgenic mouse strains which has negatively impacted the research efforts of a number of NIH-funded investigators. The opening of the NIH-funded Sanders-Brown Embryo Cryopreservation and Rederivation Core has provided the University with in-house rederivation capability for the first time, permitting the rederivation of unique transgenic rodent colonies to eliminate these opportunistic pathogens. While this facility can initially expand the colonies prior to return to the investigator, there is a lack of sufficient barier housing and support facilities on the east campus biomedical complex to isolate these rederived "clean" colonies from conventional colonies and prevent re-infection. This project has three specific aims. The first aim is the expansion of the clean cage wash/cage prep area with redirection of cage flow from the tunnel washer to eliminate a burn hazard to personnel and permit the relocation of the remotely located bedding dispenser to improve efficiency. The installation of a dry-heat sterilizer will provide the capability to support the bioexclusion barrir. The second aim is the establishment of two bioexclusion barrier animal housing rooms with a dedicated ante/procedure room. The third aim is to reduce energy and maintenance demands, improve cage microenvironment, reduce odors, and eliminate noise and vibrations by replacing the outdated Class 100 Vertical Laminar Flow (VLF) ventilation systems with a system designed to optimally support individually ventilated cage (IVC) systems. The renovation will provide the infrastructure needed for anticipated future expansion of the barrier facility and improve the flow of caging through the entire facility, improving efficiency and minimizing the potential for cross-contamination. This renovation will be the initial step in a long-term University effort to eliminae and exclude opportunistic pathogens that result in animal disease, limit the interchange of unique strains among investigators, and confound research results at the east campus biomedical complex, the university's largest animal facility.

 描述（由申请人提供）：肯塔基州大学要求50万美元与441,449美元的大学捐款相结合，通过翻新库姆斯癌症研究大楼（CCRB）动物设施的一部分，在东校区生物医学综合体上建立生物排斥屏障能力。CCRB动物设施是一个5，570平方英尺的设施，是约34，000平方英尺的连续东校区生物医学综合体动物设施的一个组成部分，包含中心设施中饲养的所有啮齿动物的笼舍卫生和灭菌支持设施。东校区生物医学综合动物设施是一个较老的设施，专为传统的动物住房。最低限度的灭菌能力和缺乏带有完整手术室的限制进入屏障饲养室严重限制了我们防止啮齿动物暴露于地方性机会性病原体（包括嗜肺巴斯德菌、螺杆菌）的能力。诺如病毒其结果是独特的免疫功能低下的转基因小鼠品系的发病率和死亡率，这对一些NIH资助的研究人员的研究工作产生了负面影响。NIH资助的Sanders-Brown胚胎冷冻保存和再衍生核心的开放首次为该大学提供了内部再衍生能力，允许对独特的转基因啮齿动物菌落进行再衍生，以消除这些机会性病原体。虽然该设施可以在返回给研究者之前最初扩大菌落，但东校区生物医学综合体缺乏足够的barier住房和支持设施，以将这些再衍生的“清洁”菌落与传统菌落隔离并防止再次感染。该项目有三个具体目标。第一个目的是扩大清洁笼清洗/笼准备区域，改变来自隧道清洗机的笼流方向，以消除人员烧伤危险，并允许重新安置远程垫料分配器，以提高效率。安装干热灭菌器将提供支持生物排斥屏障的能力。第二个目标是建立两个生物排斥屏障动物饲养室，并配备一个专用的术前/手术室。第三个目标是减少能源和维护需求，改善笼舍微环境，减少气味，并消除噪音和振动，将过时的100级垂直层流（VLF）通风系统替换为旨在最佳支持单独通风笼舍（IVC）系统的系统。翻修工程将为预计今后扩建隔离墙设施提供所需的基础设施，并改善交通流量 通过整个设施进行隔离，提高效率并最大限度地减少交叉污染的可能性。这项改造将是大学长期努力的第一步，以消除和排除导致动物疾病的机会性病原体，限制研究人员之间独特菌株的交换，并混淆东校区生物医学综合体的研究结果，该大学最大的动物设施。