Coordination of DNA Replication, Transcription and Chromatin Assembly

我们在这个项目中的工作是建立一个染色体的计算机模型，以便研究转录和复制的竞争过程。这与Aladjem博士的团队的实验工作密切合作，他们已经做了大量的测序来绘制转录和复制起始位点的分布，并测量了竞争过程的相对动力学。除了位点的空间分布外，我们基于蒙特卡罗的数学模型还考虑了细胞的调控途径对特异性、dna结合、转录和复制蛋白质复合物活性的影响。因此，该模型允许研究系统的空间、时间和调节蛋白参数变化的动力学效应，这将与实验相关。

Our part in this project is the building of a computer model of a chromosome that allows for investigating the competing processes of transcription and replication. This is in close collaboration with the experimental work of Dr. Aladjem's group, who have done extensive sequencing to map the distribution of the transcription and replication initiation sites, and measured the relative kinetics of the competing processes. In addition to the spatial distribution of sites, our Monte-Carlo-based mathematical model takes into account the effects that the Cell's regulatory pathways have on the activities of the specific, DNA-binding, transcribing and replicating protein complexes. The Model thus allows for investigating the kinetic effects of changes in the spatial, temporal and regulatory-protein parameters of the system, which will be correlated with experiment.