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Microfluidic screening devices for health-span extending drugs

用于延长健康寿命药物的微流体筛选装置

基本信息

批准号：
8647832
负责人：
SHAWN R LOCKERY
金额：
$ 15.84万
依托单位：
NEMAMETRIX, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-02-01 至 2016-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8647832
关键词：
Age Aging American Animal Model Behavior Biological Biological Assay Biological Models Biology of Aging Biomedical Engineering Businesses Caenorhabditis elegans Cessation of life Detection Development Devices Dimensions Environment Face Feeding behaviors Frequencies Genetic Goals Grant Growth Head Health Human Individual Laboratory Research Licensing Life Liquid substance Longevity Longitudinal Studies Marketing Measurement Measures Methods Microfluidic Microchips Microfluidics Microscopic Modeling Molecular Movement Nematoda Optical Methods Optics Organism Pharmaceutical Preparations Phase Population Genetics Pump Quality of life Research Research Contracts Resolution Services Signal Transduction System Technology Time Tissues United States National Institutes of Health Work age effect aged base body-mind cost innovation member movie new technology novel public health relevance research study screening seal success

项目摘要

Aging, the progressive degeneration of body and mind, is a universal problem marked by a decline in tissue organization and function, and an increase in the likelihood of death. Progress in understanding the cellular and molecular basis of aging is rapidly accelerating, following the discovery that several well-known model organisms age in many of the same ways as humans. In addition to their contributions to our basic understanding of the biology of aging, these organisms are now being used to screen for compounds that extend not only lifespan, but also the quality of life in older individuals (health span). One of the most powerful model systems in aging and health span research is the microscopic nematode Caenorhabditis elegans, favored for its short lifespan (2-3 weeks), genetic tractability, and low cost. C. elegans research is currently undergoing rapid innovation as its small size is particularly compatible with microfluidics - the manipulation of fluids in channels with dimensions of tens of micrometers. The overall goal of the proposed research is to accelerate the discovery of compounds that extend health span through the development of a novel microfluidic device that is likely to enhance the throughput and resolution of health span screening in this key organism. The conventional method of health span screening in C. elegans currently faces three critical barriers: the absence of rigorously standardized growth conditions, the difficulty of making repeated measurements on identified individuals, and the challenge of high-throughput quantification of feeding behavior, one of the most reliable measures of health in the worm. The proposed research takes a head-on approach to these challenges by combining, into a single device, two previously established microfluidic technologies, one for long-term culture of large numbers of individually isolated worms, and one for making non-invasive electrical recordings of feeding behavior. Phase I of the proposed research seeks fundamental proof of concept for the combined device. Phase II of the project extends this work by automating health- span screens and increasing their throughput.

衰老是身体和精神的逐渐退化，是一个以组织衰退为特征的普遍问题