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Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules

使用无毒病毒纳米颗粒来计数癌症生物标志物分子

基本信息

批准号：
8873755
负责人：
Chuanbin Mao
金额：
$ 16.36万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8873755
关键词：
Affect Bacteria Bacteriophage T7 Bacteriophages Binding Biological Markers Body Fluids Buffers Cancer Patient Capsid Cataloging Catalogs Clinical Color Complement Complementary DNA Complex DNA Detection Diagnosis Engineering Eye Family Fiber Fluorescence Gene Expression Genetic Engineering Genetic Transcription Goals Gold Human Infection Lead Magnetism Malignant Neoplasms Malignant neoplasm of prostate Matrix Metalloproteinases Methods MicroRNAs Nanotechnology Oligonucleotides Patients Peptides Phage Display Polymerase Chain Reaction Property Proteins Public Health RNA Recombinants Reporting Reproducibility Research Reverse Transcription Risk Sampling Series Serum Sodium Chloride Surface Tail Techniques Testing Text Time Tissues Tumor Tissue Untranslated RNA Virus Water abstracting base cancer diagnosis cancer type circulating microRNA cost design improved nanoparticle novel public health relevance

项目摘要

Abstract 1 R21 CA195607-01 Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules Modified Project Summary/Abstract Section Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. A type of biomarkers for prostate cancer, called microRNAs (miRNAs), has a unique level in serum from cancer patients. Hence, detecting them will allow us to diagnose prostate cancer earlier. Currently, this type of biomarkers can only be detected by quantitative real-time polymerase chain reaction. However, this technique does not generate reproducible and reliable results when it is used to detect miRNAs at a low level. To be able to reliably quantify prostate cancer-specific miRNAs at a low level, we propose an ultrasensitive strategy that hires non-toxic virus nanoparticles for probing the miRNAs. This strategy is based on the fact that the virus nanoparticles can be engineered to become capable of emitting three different lights and form complex with nanoparticles for capturing the target miRNAs. It is thus possible to detect multiple target miRNAs in one platform because the virus nanoparticles can emit three different fluorescence lights for detecting three different target miRNAs when three different fluorescent molecules are presented on the surface of the virus nanoparticles. Our objective in this project is to develop the new strategy in order to quantify multiple prostate cancer miRNA biomarkers in serum samples, including three target miRNAs specific for the prostate cancer. We aim to achieve high sensitivity, accuracy, and reproducibility for detecting the prostate cancer biomarkers in serum. This project will develop a new facile method that can precisely detect the prostate cancer biomarkers and thus can be used for early prostate cancer diagnosis.

摘要