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Hiring Non-toxic Virus Nanoparticles to Count Cancer Biomarker Molecules

使用无毒病毒纳米颗粒来计数癌症生物标志物分子

基本信息

批准号：
9070724
负责人：
Chuanbin Mao
金额：
$ 19.52万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9070724
关键词：
Affect Bacteria Bacteriophage T7 Bacteriophages Binding Biological Markers Body Fluids Buffers Cancer Patient Capsid Cataloging Catalogs Clinical Color Complement Complementary DNA Complex DNA Detection Diagnosis Engineering Eye Family Fiber Fluorescence Gene Expression Genetic Engineering Genetic Transcription Goals Gold Health Human Infection Light Magnetic nanoparticles Magnetism Malignant Neoplasms Malignant neoplasm of prostate Methods MicroRNAs Nanotechnology Oligonucleotides Patients Peptides Phage Display Polymerase Chain Reaction Property Proteins RNA Recombinants Reporting Reproducibility Reverse Transcription Risk Sampling Series Serum Sodium Chloride Structure Surface Tail Techniques Testing Time Tissues Tumor Tissue Untranslated RNA Virus Water base cancer biomarkers cancer diagnosis cancer type circulating microRNA cost design improved microRNA biomarkers nanoparticle novel specific biomarkers

项目摘要

A type of biomarkers for prostate cancer, called microRNAs (miRNAs), has a unique level in serum from cancer patients. Hence, detecting them will allow us to diagnose prostate cancer earlier. Currently, this type of biomarkers can only be detected by quantitative real-time polymerase chain reaction. However, this technique does not generate reproducible and reliable results when it is used to detect miRNAs at a low level. To be able to reliably quantify prostate cancer-specific miRNAs at a low level, we propose an ultrasensitive strategy that hires non-toxic virus nanoparticles for probing the miRNAs. This strategy is based on the fact that the virus nanoparticles can be engineered to become capable of emitting three different lights and form complex with nanoparticles for capturing the target miRNAs. It is thus possible to detect multiple target miRNAs in one platform because the virus nanoparticles can emit three different fluorescence lights for detecting three different target miRNAs when three different fluorescent molecules are presented on the surface of the virus nanoparticles. Our objective in this project is to develop the new strategy in order to quantify multiple prostate cancer miRNA biomarkers in serum samples, including three target miRNAs specific for the prostate cancer. We aim to achieve high sensitivity, accuracy, and reproducibility for detecting the prostate cancer biomarkers in serum. This project will develop a new facile method that can precisely detect the prostate cancer biomarkers and thus can be used for early prostate cancer diagnosis.

前列腺癌的一种生物标志物称为 microRNA (miRNA)，在癌症患者的血清中具有独特的水平。因此，检测它们将使我们能够更早地诊断前列腺癌。目前，此类生物标志物只能通过定量实时聚合酶链反应来检测。然而，当该技术用于检测低水平的 miRNA 时，无法产生可重复且可靠的结果。为了能够可靠地定量低水平的前列腺癌特异性 miRNA，我们提出了一种超灵敏策略，采用无毒的病毒纳米粒子来探测 miRNA。该策略基于以下事实：病毒纳米颗粒可以被设计为能够发射三种不同的光，并与纳米颗粒形成复合物以捕获目标 miRNA。由于当病毒纳米颗粒表面存在三种不同的荧光分子时，病毒纳米颗粒可以发出三种不同的荧光来检测三种不同的目标miRNA，因此可以在一个平台中检测多个目标miRNA。我们在这个项目中的目标是开发新策略来量化血清样本中的多种前列腺癌 miRNA 生物标志物，包括前列腺癌特异性的三种目标 miRNA。我们的目标是实现检测血清中前列腺癌生物标志物的高灵敏度、准确性和重现性。该项目将开发一种新的简便方法，可以精确检测前列腺癌生物标志物，从而可用于早期前列腺癌诊断。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Ti nanorod arrays with a medium density significantly promote osteogenesis and osteointegration.

DOI：
10.1038/srep19047
发表时间：
2016-01-08
期刊：
Scientific reports
影响因子：
4.6
作者：
Ning C;Wang S;Zhu Y;Zhong M;Lin X;Zhang Y;Tan G;Li M;Yin Z;Yu P;Wang X;Li Y;He T;Chen W;Wang Y;Mao C
通讯作者：
Mao C

Cross Talk Between Autophagy and Apoptosis Contributes to ZnO Nanoparticle-Induced Human Osteosarcoma Cell Death.

自噬和细胞凋亡之间的相互作用导致氧化锌纳米粒子诱导的人骨肉瘤细胞死亡

DOI：
10.1002/adhm.201800332
发表时间：
2018-09
期刊：
Advanced healthcare materials
影响因子：
10
作者：
He G;Ma Y;Zhu Y;Yong L;Liu X;Wang P;Liang C;Yang C;Zhao Z;Hai B;Pan X;Liu Z;Liu X;Mao C
通讯作者：
Mao C

Phage as a Genetically Modifiable Supramacromolecule in Chemistry, Materials and Medicine.

DOI：
10.1021/acs.accounts.5b00557
发表时间：
2016-06-21
期刊：
Accounts of chemical research
影响因子：
18.3
作者：
Cao B;Yang M;Mao C
通讯作者：
Mao C

Bone-Inspired Spatially Specific Piezoelectricity Induces Bone Regeneration.

受骨启发的空间特异性压电诱导骨再生

DOI：
10.7150/thno.19748
发表时间：
2017
期刊：
Theranostics
影响因子：
12.4
作者：
Yu P;Ning C;Zhang Y;Tan G;Lin Z;Liu S;Wang X;Yang H;Li K;Yi X;Zhu Y;Mao C
通讯作者：
Mao C

Corrigendum to "Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels" [Biomaterials 188 (2019) 130-143].

“通过水凝胶解开骨肿瘤细胞和骨形成细胞对基质硬度和粘附配体密度的反应”的勘误表[Biomaterials 188 (2019) 130-143]。

DOI：
10.1016/j.biomaterials.2019.119663
发表时间：
2020
期刊：
Biomaterials
影响因子：
14
作者：
Jiang,Tongmeng;Zhao,Jinmin;Yu,Shan;Mao,Zhengwei;Gao,Changyou;Zhu,Ye;Mao,Chuanbin;Zheng,Li
通讯作者：
Zheng,Li