Molecular Genetics of Segment Identity

片段同一性的分子遗传学

• 批准号: 8637078
• 负责人: RICHARD S MANN
• 金额: $ 35.97万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8637078
• 关键词: Abdomen; Adult; Amino Acid Motifs; Animals; Anterior; Architecture; Attention; Binding; Binding Sites; Biological Models; Cell Maintenance; ChIP-on-chip; Chest; Chromatin; Chromatin Structure; Comb animal structure; Complement; Complex; DNA; DNA Binding; Data; Development; Dorsal; Drosophila genus; Drosophila melanogaster; Elements; Embryo; Family; Funding; Gene Expression Profile; Gene Targeting; Genes; Genome; Goals; Homeodomain Proteins; In Vitro; Individual; Leg; Maps; Molecular Genetics; Motor Neurons; Pattern; Phenotype; Play; Property; Protein Binding; Proteins; Recruitment Activity; Regulation; Regulatory Element; Resolution; Role; Specificity; Staging; Stem cells; Structure; Technology; Testing; Time; Tissues; Transgenes; Vertebrates; Work; base; cell type; cofactor; fly; homeodomain; imaginal disc; in vivo; insight; mutant; novel; preference; protein function; research study; sex; transcription factor

DESCRIPTION (provided by applicant): The Hox genes encode a conserved family of homeodomain-containing transcriptional regulators that are critical for many aspects of animal development, in all metazoans. The long-term goal of this project is to understand how these proteins function as transcriptional regulators at a mechanistic level. Three approaches will be used: 1) a structure-function analysis of three Hox proteins, using an in vivo, rescue-based strategy, focusing especially on Hox protein motifs that interact with Hox cofactors in a context-dependent manner, 2) the high-resolution characterization of individual Hox- targeted regulatory elements, and 3) an analysis of the Hox-dependent chromatin architectures in the imaginal discs, the precursors of the adult fly. Together, these studies will provide important insights int how these and other transcriptional regulators carry out their specific functions during animal development.

描述(申请人提供)：HOX基因编码一个保守的含有同源结构域的转录调节因子家族，在所有后生动物中对动物发育的许多方面都是关键的。这个项目的长期目标是了解这些蛋白质如何在机制水平上作为转录调节因子发挥作用。我们将使用三种方法：1)使用在体的、基于救援的策略对三种HOX蛋白的结构和功能进行分析，特别是关注以上下文相关的方式与HOX辅助因子相互作用的HOX蛋白基序；2)高分辨率地表征单个HOX靶向调控元件；以及3)分析成虫的前体--成虫视盘中依赖于HOX的染色质结构。总之，这些研究将为这些和其他转录调控因子如何在动物发育过程中执行其特定功能提供重要的见解。