Leica TCS SP8 Confocal Microscope System

 描述（由申请人提供）：本申请的目标是获得最先进的激光扫描共聚焦显微镜，以支持杜兰国家灵长类动物研究中心（TNPRC）的11名NIH资助的研究人员的研究。TNPRC是NIH支持的国家资源，其使命是整合生物医学研究中的研究、培训和服务，通过基础和应用生物医学研究改善人类和动物健康。现有的多用户共焦系统，一个徕卡TCS SP2购买于2002年已经连续运行了十多年，并提供了出色的服务，研究社区。虽然Leica TCS SP2在购买时是最先进的，但该系统不再满足我们在多标记成像研究、光敏性、成像质量、速度和多功能性方面的需求。此外，徕卡将在明年内停止为SP2提供服务。在本申请中，我们介绍了来自11名研究人员（8名主要用户和3名次要用户）的研究项目，他们的工作是在TNPRC进行的。这些项目主要侧重于传染病和免疫学，这是TNPRC的特殊专业领域，吸引了国家和国际合作者。每个用户都有强大的NIH资助历史和对先进成像技术的需求。我们建议购买Leica TCS SP 8五通道共聚焦显微成像系统，配有倒置显微镜，6条强激光线，（二极管：405 nm，蓝Ar：458、488、514 nm，黄色二极管：561 nm，和红色HeNe：633 nm），AOBS，和两个AOTF（405 nm和可见光），3个混合GaAsP光谱探测器和2个HS PMT和易于使用的先进软件，所有这些都是必不可少的项目的用户。与市场上的其他一流系统相比，我们认为Leica TCS SP 8在质量、选项、服务和价格方面提供了最佳的整体系统。它还具有高度灵活性，能够根据用户的需要提供新的服务。这种新的共聚焦显微镜系统是必不可少的，不仅是在应用程序中列出的主要和次要用户，但对TNPRC的使命作为一个国家资源，使用非人灵长类动物的生物医学研究。一个更新的，更可靠的，复杂的和多功能的仪器，如SP 8的可用性可能会吸引更多的研究人员，需要这种技术，导致新的合作，进一步扩大非人类灵长类动物研究的范围在TNPRC。

 DESCRIPTION (provided by applicant): This goal of this application is to acquire a state-of-the-art laser-scanning confocal microscope to support the research of 11 NIH-funded investigators at the Tulane National Primate Research Center (TNPRC). The TNPRC is an NIH-supported national resource with a mission that integrates research, training and service in biomedical research to improve human and animal health through basic and applied biomedical research. The existing multiuser confocal system, a Leica TCS SP2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research community. While the Leica TCS SP2 was state-of-the-art when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity, imaging quality, speed, and versatility. Furthermore, Leica will cease to provide service for the SP2 within the next year. In this application we present research projects from 11 investigators (8 Major users and 3 Minor users) whose work is performed at the TNPRC. These projects are primarily focused on infectious diseases and immunology which are areas of exceptional expertise at the TNPRC and that attract national and international collaborators. Each of the users has a strong history of NIH funding and needs for advanced imaging technology. We propose purchasing a Leica TCS SP 8 five-channel confocal microscopy imaging system with an inverted microscope, 6 strong laser lines (diode: 405 nm, Blue Ar: 458, 488, 514 nm, yellow diode: 561 nm, and red HeNe: 633 nm) with AOBS, and two AOTF (405 nm and visible), 3 hybrid GaAsP spectral detectors and 2 HS PMT and easy to use advanced software all of which are essential to the projects of the users. Compared to other premiere systems on the market we feel the Leica TCS SP 8 offers the best overall system in terms of quality, options, service, and price. It also is highly flexible and will be able to provide new services as required by the users. This new confocal microscope system is essential not only to the major and minor users listed in the application but to the mission of the TNPRC as a national resource for biomedical research using nonhuman primates. The availability of a newer, more reliable, sophisticated and versatile instrument such as the SP 8 is likely to attract additional investigators that require ths technology leading to new collaborations further expanding the scope of nonhuman primate research at the TNPRC.