Leica TCS SP8 Confocal Microscope System

Leica TCS SP8 共焦显微镜系统

• 批准号: 8825740
• 负责人: ANDREW A LACKNER
• 金额: $ 47.78万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825740
• 关键词: Animals; Area; Biomedical Research; Collaborations; Communities; Computer software; Confocal Microscopy; Funding; Goals; Health; Human; Hybrids; Image; Imaging technology; Infectious Disease Immunology; International; Lasers; Marketing; Microscope; Minor; Mission; Photosensitivity; Price; Primates; Recording of previous events; Research; Research Personnel; Research Project Grants; Research Support; Research Training; Resources; Scanning; Services; Speed; System; Technology; United States National Institutes of Health; Work; detector; flexibility; imaging system; improved; instrument; meetings; nonhuman primate; operation

 DESCRIPTION (provided by applicant): This goal of this application is to acquire a state-of-the-art laser-scanning confocal microscope to support the research of 11 NIH-funded investigators at the Tulane National Primate Research Center (TNPRC). The TNPRC is an NIH-supported national resource with a mission that integrates research, training and service in biomedical research to improve human and animal health through basic and applied biomedical research. The existing multiuser confocal system, a Leica TCS SP2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research community. While the Leica TCS SP2 was state-of-the-art when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity, imaging quality, speed, and versatility. Furthermore, Leica will cease to provide service for the SP2 within the next year. In this application we present research projects from 11 investigators (8 Major users and 3 Minor users) whose work is performed at the TNPRC. These projects are primarily focused on infectious diseases and immunology which are areas of exceptional expertise at the TNPRC and that attract national and international collaborators. Each of the users has a strong history of NIH funding and needs for advanced imaging technology. We propose purchasing a Leica TCS SP 8 five-channel confocal microscopy imaging system with an inverted microscope, 6 strong laser lines (diode: 405 nm, Blue Ar: 458, 488, 514 nm, yellow diode: 561 nm, and red HeNe: 633 nm) with AOBS, and two AOTF (405 nm and visible), 3 hybrid GaAsP spectral detectors and 2 HS PMT and easy to use advanced software all of which are essential to the projects of the users. Compared to other premiere systems on the market we feel the Leica TCS SP 8 offers the best overall system in terms of quality, options, service, and price. It also is highly flexible and will be able to provide new services as required by the users. This new confocal microscope system is essential not only to the major and minor users listed in the application but to the mission of the TNPRC as a national resource for biomedical research using nonhuman primates. The availability of a newer, more reliable, sophisticated and versatile instrument such as the SP 8 is likely to attract additional investigators that require ths technology leading to new collaborations further expanding the scope of nonhuman primate research at the TNPRC.

 描述（由申请人提供）：本申请的目标是获得一台最先进的激光扫描共焦显微镜，以支持 11 名 NIH 资助的研究人员在杜兰国家灵长类动物研究中心 (TNPRC) 的研究。 TNPRC 是 NIH 支持的国家资源，其使命是将生物医学研究的研究、培训和服务结合起来，通过基础和应用生物医学研究改善人类和动物健康。现有的多用户共焦系统（2002 年购买的 Leica TCS SP2）已连续运行十多年，为研究界提供了出色的服务。虽然 Leica TCS SP2 在购买时是最先进的，但该系统不再满足我们在多标签成像研究、光敏性、成像质量、速度和多功能性方面的需求。此外，徕卡将在明年停止为 SP2 提供服务。在此应用程序中，我们展示了 11 位研究人员（8 位主要用户和 3 位次要用户）的研究项目，他们的工作在 TNPRC 进行。这些项目主要集中于传染病和免疫学，这些领域是 TNPRC 的特殊专业领域，吸引了国内外合作者。每个用户都有 NIH 资助的悠久历史和对先进成像技术的需求。我们建议购买 Leica TCS SP 8 五通道共焦显微成像系统，配备倒置显微镜、6 条强激光线（二极管：405 nm、蓝色 Ar：458、488、514 nm、黄色二极管：561 nm 和红色 HeNe：633 nm），带 AOBS、两个 AOTF（405 nm 和可见光）、3 个混合 GaAsP 光谱探测器和 2 个 HS PMT 以及易于使用的高级软件，所有这些对于用户的项目都是必不可少的。与市场上的其他一流系统相比，我们认为 Leica TCS SP 8 在质量、选项、服务和价格方面提供了最好的整体系统。它还具有高度的灵活性，能够根据用户的需求提供新的服务。这种新型共焦显微镜系统不仅对于申请中列出的主要和次要用户至关重要，而且对于 TNPRC 作为使用非人类灵长类动物进行生物医学研究的国家资源的使命至关重要。 SP 8 等更新、更可靠、更先进和多功能的仪器的出现可能会吸引更多需要该技术的研究人员，从而促成新的合作，进一步扩大 TNPRC 非人类灵长类动物研究的范围。