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BioMark HD System for High-Throughput Genomic Studies

用于高通量基因组研究的 BioMark HD 系统

基本信息

批准号：
8826492
负责人：
Ning Jenny Jiang
金额：
$ 48.94万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-03-01 至 2017-10-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Project Abstract This proposal requests support for the purchase of a microfluidic chip based qPCR system - Biomark HD with the Access Array module and the C1 Single Cell AutoPrep System as a shared resource to benefit major NIH supported projects at the University of Texas at Austin. The Biomark HD reader is a real-time PCR instrument designed to run Fluidigm Integrated Fluidic Circuits (IFCs) in either real-time or end-point read modes. This system has revolutionized real-time PCR field and enabled many next generation genomic studies that otherwise was impossible. Furthermore, the easy use interface allows the integration of high-throughput genomic study into many areas of research and many other interfaces, such as systems immunology, regenerative medicine, cancer imaging, mutant detection and screening, and rare cell imaging. The proposed Biomark HD system will have applications in four main areas: 1) target enrichment and next-generation-sequencing library preparation 2) single cell gene expression and gene expression, 3) multiplexed genome typing and 4) molecular counting using digital PCR. The following 5 research areas will be benefited tremendously from these applications offered by BioMark HD system: 1) immune repertoire profiling, 2) nerve regeneration and neural tube defects, 3) cancer biology, 4) vascular tissue engineering, 5) transcription regulation, chromosome organization, and repair, and 6) evolution biology. These areas of research are exemplified in the projects that proposed by the users. The versatility of the instrument not only helps users to complete the existing projects funded by NIH, but also allows users to formulate and test new hypotheses that otherwise would be impossible. Despite the large number of scientists that have an overwhelming need to have access to the Biomark HD system, currently, there is no such a shared BioMark HD system on UT Austin campus. The use of this instrument will impact a wide range of NIH funded projects on both basic science as well as translational research. The addition of this instrument to the University of Texas will directly impact the specific NIH funded research projects while also provide a critical research tool to the broader biomedical engineering and science community on campus.

描述（由申请人提供）：项目摘要本提案请求支持购买基于微流控芯片的 qPCR 系统 - Biomark HD（带有 Access Array 模块）和 C1 单细胞 AutoPrep 系统作为共享资源，以使 NIH 支持的德克萨斯大学奥斯汀分校的主要项目受益。 Biomark HD 阅读器是一款实时 PCR 仪器，设计用于在实时或终点读取模式下运行 Fluidigm 集成流体电路 (IFC)。该系统彻底改变了实时 PCR 领域，使许多原本不可能进行的下一代基因组研究成为可能。此外，易于使用的界面允许将高通量基因组研究集成到许多研究领域和许多其他界面中，例如系统免疫学、再生医学、癌症成像、突变体检测和筛选以及稀有细胞成像。拟议的 Biomark HD 系统将应用于四个主要领域：1）靶点富集和下一代测序文库制备2）单细胞基因表达和基因表达，3）多重基因组分型和4）使用数字PCR的分子计数。以下 5 个研究领域将从 BioMark HD 系统提供的这些应用中受益匪浅：1) 免疫组库分析，2) 神经再生和神经管缺陷，3) 癌症生物学，4) 血管组织工程，5) 转录调控、染色体组织和修复，以及 6) 进化生物学。这些研究领域在用户提出的项目中得到了例证。该仪器的多功能性不仅可以帮助用户完成 NIH 资助的现有项目，还可以让用户制定和测试原本不可能的新假设。尽管大量科学家迫切需要访问 Biomark HD 系统，但目前 UT 奥斯汀校区还没有这样的共享 BioMark HD 系统。该仪器的使用将影响 NIH 资助的基础科学和转化研究项目。该仪器添加到德克萨斯大学将直接影响 NIH 资助的具体研究项目，同时也为校园内更广泛的生物医学工程和科学界提供重要的研究工具。