BioMark HD System for High-Throughput Genomic Studies

 描述（由申请人提供）： 项目摘要该提案要求支持购买基于微流控芯片的qPCR系统- Biomark HD，该系统具有Access Array模块和C1单细胞AutoPrep系统，作为共享资源，以使德克萨斯大学奥斯汀分校的主要NIH支持项目受益。 Biomark HD读取器是一种实时PCR仪器，设计用于以实时或终点读取模式运行Fluidigm集成荧光回路（IFC）。该系统彻底改变了实时PCR领域，并使许多下一代基因组研究成为可能。此外，易于使用的界面允许将高通量基因组研究整合到许多研究领域和许多其他界面中，例如系统免疫学，再生医学，癌症成像，突变检测和筛选以及稀有细胞成像。所提出的Biomark HD系统将在四个主要领域中应用：1）目标富集和下一代测序文库制备2）单细胞基因表达和基因表达，3）多重基因组分型和4）使用数字PCR的分子计数。以下5个研究领域将从BioMark HD系统提供的这些应用中受益匪浅：1）免疫谱分析，2）神经再生和神经管缺陷，3）癌症生物学，4）血管组织工程，5）转录调控，染色体组织和修复，以及6）进化生物学。这些领域的研究都体现在用户提出的项目。该仪器的多功能性不仅可以帮助用户完成NIH资助的现有项目，还可以让用户制定和测试新的假设，否则将是不可能的。 尽管有大量的科学家，有一个压倒性的需要有访问的Biomark HD系统，目前，有没有这样的共享BioMark HD系统在UT奥斯汀校园。该工具的使用将影响NIH资助的基础科学和转化研究项目。该仪器的加入将直接影响美国国立卫生研究院资助的特定研究项目，同时也为校园内更广泛的生物医学工程和科学界提供了一个重要的研究工具。

 DESCRIPTION (provided by applicant): Project Abstract This proposal requests support for the purchase of a microfluidic chip based qPCR system - Biomark HD with the Access Array module and the C1 Single Cell AutoPrep System as a shared resource to benefit major NIH supported projects at the University of Texas at Austin. The Biomark HD reader is a real-time PCR instrument designed to run Fluidigm Integrated Fluidic Circuits (IFCs) in either real-time or end-point read modes. This system has revolutionized real-time PCR field and enabled many next generation genomic studies that otherwise was impossible. Furthermore, the easy use interface allows the integration of high-throughput genomic study into many areas of research and many other interfaces, such as systems immunology, regenerative medicine, cancer imaging, mutant detection and screening, and rare cell imaging. The proposed Biomark HD system will have applications in four main areas: 1) target enrichment and next-generation-sequencing library preparation 2) single cell gene expression and gene expression, 3) multiplexed genome typing and 4) molecular counting using digital PCR. The following 5 research areas will be benefited tremendously from these applications offered by BioMark HD system: 1) immune repertoire profiling, 2) nerve regeneration and neural tube defects, 3) cancer biology, 4) vascular tissue engineering, 5) transcription regulation, chromosome organization, and repair, and 6) evolution biology. These areas of research are exemplified in the projects that proposed by the users. The versatility of the instrument not only helps users to complete the existing projects funded by NIH, but also allows users to formulate and test new hypotheses that otherwise would be impossible. Despite the large number of scientists that have an overwhelming need to have access to the Biomark HD system, currently, there is no such a shared BioMark HD system on UT Austin campus. The use of this instrument will impact a wide range of NIH funded projects on both basic science as well as translational research. The addition of this instrument to the University of Texas will directly impact the specific NIH funded research projects while also provide a critical research tool to the broader biomedical engineering and science community on campus.